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Micromachines
Volume 10
Issue 9
10.3390/mi10090575
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Article

Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

by
Gorachand Dutta
1,2,*,
Abdoulie A. Jallow
3,
Debjani Paul
3,* and
Despina Moschou
1,*
1
Centre for Biosensors, Bioelectronics and Biodevices (C3Bio), Department of Electronic & Electrical Engineering, University of Bath, Bath BA2 7AY, UK
2
School of Medical Science and Technology (SMST), Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India
3
Biosciences and Bioengineering Department, Indian Institute of Technology Bombay, Powai 400076, India
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(9), 575; https://doi.org/10.3390/mi10090575
Submission received: 23 May 2019 / Revised: 4 July 2019 / Accepted: 15 July 2019 / Published: 30 August 2019
(This article belongs to the Special Issue Microfluidics on Printed Circuit Boards)
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Abstract

This paper reports for the first time printed-circuit-board (PCB)-based label-free electrochemical detection of bacteria. The demonstrated immunosensor was implemented on a PCB sensing platform which was designed and fabricated in a standard PCB manufacturing facility. Bacteria were directly captured on the PCB sensing surface using a specific, pre-immobilized antibody. Electrochemical impedance spectra (EIS) were recorded and used to extract the charge transfer resistance (Rct) value for the different bacteria concentrations under investigation. As a proof-of-concept, Streptococcus mutans (S. mutans) bacteria were quantified in a phosphate buffered saline (PBS) buffer, achieving a limit of detection of 103 CFU/mL. Therefore, the proposed biosensor is an attractive candidate for the development of a simple and robust point-of-care diagnostic platform for bacteria identification, exhibiting good sensitivity, high selectivity, and excellent reproducibility.
Keywords: electrochemical impedance spectroscopy; immunosensor; direct bacteria detection; lab-on-PCB (printed-circuit-board); Streptococcus mutans (S. mutans) electrochemical impedance spectroscopy; immunosensor; direct bacteria detection; lab-on-PCB (printed-circuit-board); Streptococcus mutans (S. mutans)

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MDPI and ACS Style

Dutta, G.; Jallow, A.A.; Paul, D.; Moschou, D. Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes. Micromachines 2019, 10, 575. https://doi.org/10.3390/mi10090575

AMA Style

Dutta G, Jallow AA, Paul D, Moschou D. Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes. Micromachines. 2019; 10(9):575. https://doi.org/10.3390/mi10090575

Chicago/Turabian Style

Dutta, Gorachand, Abdoulie A. Jallow, Debjani Paul, and Despina Moschou. 2019. "Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes" Micromachines 10, no. 9: 575. https://doi.org/10.3390/mi10090575

APA Style

Dutta, G., Jallow, A. A., Paul, D., & Moschou, D. (2019). Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes. Micromachines, 10(9), 575. https://doi.org/10.3390/mi10090575

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