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Hematology Reports
Volume 10
Issue 3
10.4081/hr.2018.7576
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Hematology Reports is published by MDPI from Volume 14 Issue 1 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
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Brief Report

NOTCH Activation Promotes Glycosyltransferase Expression in Human Myeloid Leukemia Cells

by
Shichun Wang
,
Mai Itoh
,
Erika Shiratori
,
Mika Ohtaka
and
Shuji Tohda
*
Department of Laboratory Medicine, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo- Ku, Tokyo 113-8519, Japan
*
Author to whom correspondence should be addressed.
Hematol. Rep. 2018, 10(3), 7576; https://doi.org/10.4081/hr.2018.7576
Submission received: 9 January 2018 / Revised: 13 August 2018 / Accepted: 22 August 2018 / Published: 24 September 2018

Abstract

NOTCH signaling diversely regulates the growth of acute myeloid leukemia (AML) cells. It is known that glycosylation of NOTCH receptors modulates NOTCH activation. However, little is known about glycosylation of NOTCH in AML cells. We examined the effects of ligand-induced NOTCH activation on the expression of NOTCH-modifying glycosyltransferases in two AML cell lines, THP-1 and TMD7. The cells were stimulated with recombinant NOTCH ligands JAGGED1 and DELTA1, and subjected to immunoblot analysis to evaluate the expression levels of glycosyltransferases. Ligand stimulation promoted the expression of POFUT1, LFNG, MFNG, RFNG, GXYLT1, GXYLT2, and XXYLT1 in THP-1 cells, and that of RFNG and GXYLT1 in TMD7 cells. We found that NOTCH activation promoted the expression of several glycosyltransferases in AML cells. This suggests that NOTCH activation modulates its sensitivity to NOTCH ligands by increased glycosylation of NOTCH receptors in AML cells. Further investigation is needed to elucidate its biological significance.
Keywords: acute myeloid leukemia; NOTCH; glycosyltransferase acute myeloid leukemia; NOTCH; glycosyltransferase

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MDPI and ACS Style

Wang, S.; Itoh, M.; Shiratori, E.; Ohtaka, M.; Tohda, S. NOTCH Activation Promotes Glycosyltransferase Expression in Human Myeloid Leukemia Cells. Hematol. Rep. 2018, 10, 7576. https://doi.org/10.4081/hr.2018.7576

AMA Style

Wang S, Itoh M, Shiratori E, Ohtaka M, Tohda S. NOTCH Activation Promotes Glycosyltransferase Expression in Human Myeloid Leukemia Cells. Hematology Reports. 2018; 10(3):7576. https://doi.org/10.4081/hr.2018.7576

Chicago/Turabian Style

Wang, Shichun, Mai Itoh, Erika Shiratori, Mika Ohtaka, and Shuji Tohda. 2018. "NOTCH Activation Promotes Glycosyltransferase Expression in Human Myeloid Leukemia Cells" Hematology Reports 10, no. 3: 7576. https://doi.org/10.4081/hr.2018.7576

APA Style

Wang, S., Itoh, M., Shiratori, E., Ohtaka, M., & Tohda, S. (2018). NOTCH Activation Promotes Glycosyltransferase Expression in Human Myeloid Leukemia Cells. Hematology Reports, 10(3), 7576. https://doi.org/10.4081/hr.2018.7576

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