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Viruses 2018, 10(9), 469; https://doi.org/10.3390/v10090469

Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification

Department of Molecular Biology and Plant Virology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, 70550 Stuttgart, Germany
Received: 2 August 2018 / Revised: 28 August 2018 / Accepted: 30 August 2018 / Published: 31 August 2018
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

The experience with a diagnostic technology based on rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) analyses, and direct or deep sequencing (Circomics) over the past 15 years is surveyed for the plant infecting geminiviruses, nanoviruses and associated satellite DNAs, which have had increasing impact on agricultural and horticultural losses due to global transportation and recombination-aided diversification. Current state methods for quarantine measures are described to identify individual DNA components with great accuracy and to recognize the crucial role of the molecular viral population structure as an important factor for sustainable plant protection. View Full-Text
Keywords: geminivirus; nanovirus; satellite; RCA; RFLP; Circomics geminivirus; nanovirus; satellite; RCA; RFLP; Circomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jeske, H. Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification. Viruses 2018, 10, 469.

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