Next Article in Journal
Sub-Micron Particle Based Structures as Reconfigurable Photonic Devices Controllable by External Photonic and Magnetic Fields
Previous Article in Journal
Wireless Remote Weather Monitoring System Based on MEMS Technologies
Article Menu

Export Article

Open AccessArticle
Sensors 2011, 11(3), 2728-2739;

Subtractive Inhibition Assay for the Detection of E. coli O157:H7 Using Surface Plasmon Resonance

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, Zhejiang, China
Author to whom correspondence should be addressed.
Received: 8 January 2011 / Revised: 29 January 2011 / Accepted: 21 February 2011 / Published: 1 March 2011
(This article belongs to the Section Biosensors)
Full-Text   |   PDF [286 KB, uploaded 21 June 2014]   |  


A surface plasmon resonance (SPR) immunosensor was developed for the detection of E. coli O157:H7 by means of a new subtractive inhibition assay. In the subtractive inhibition assay, E. coli O157:H7 cells and goat polyclonal antibodies for E. coli O157:H7 were incubated for a short of time, and then the E. coli O157:H7 cells which bound antibodies were removed by a stepwise centrifugation process. The remaining free unbound antibodies were detected through interaction with rabbit anti-goat IgG polyclonal antibodies immobilized on the sensor chip using a BIAcore 3000 biosensor. The results showed that the signal was inversely correlated with the concentration of E. coli O157:H7 cells in a range from 3.0 × 104 to 3.0 × 108 cfu/mL with a detection limit of 3.0 × 104 cfu/mL. Compared with direct SPR by immobilizing antibodies on the chip surface to capture the bacterial cells and ELISA for E. coli O157:H7 (detection limit: both 3.0 × 105 cfu/mL in this paper), the detection limit of subtractive inhibition assay method was reduced by one order of magnitude. The method simplifies bacterial cell detection to protein-protein interaction, which has the potential for providing a practical alternative for the monitoring of E. coli O157:H7 and other pathogens. View Full-Text
Keywords: subtractive inhibition assay; SPR; E. coli O157:H7 subtractive inhibition assay; SPR; E. coli O157:H7

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Wang, Y.; Ye, Z.; Si, C.; Ying, Y. Subtractive Inhibition Assay for the Detection of E. coli O157:H7 Using Surface Plasmon Resonance. Sensors 2011, 11, 2728-2739.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top