Next Article in Journal
Metabolic Syndrome and Reproduction
Next Article in Special Issue
Surface Topography of Titanium Affects Their Osteogenic Potential through DNA Methylation
Previous Article in Journal
Regorafenib-Attenuated, Bleomycin-Induced Pulmonary Fibrosis by Inhibiting the TGF-β1 Signaling Pathway
Previous Article in Special Issue
Relevance of Notch Signaling for Bone Metabolism and Regeneration
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 4
10.3390/ijms22041987
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation

by
Chung-Min Kang
1,2,†,
Seunghan Mo
1,†,
Mijeong Jeon
1,2,
Ui-Won Jung
3,
Yooseok Shin
2,4,
Jin-Su Shin
5,
Bo-Young Shin
5,
Sang-Kyou Lee
5,
Hyung-Jun Choi
1,2,* and
Je Seon Song
1,2,*
1
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Korea
2
Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea
3
Department of Periodontology, College of Dentistry, Yonsei University, Seoul 03722, Korea
4
Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Korea
5
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea
*
Authors to whom correspondence should be addressed.
†
These authors equally contributed to this work.
Int. J. Mol. Sci. 2021, 22(4), 1987; https://doi.org/10.3390/ijms22041987
Submission received: 15 December 2020 / Revised: 14 February 2021 / Accepted: 16 February 2021 / Published: 17 February 2021
(This article belongs to the Special Issue Bone Development and Regeneration)
Browse Figures
Review Reports Versions Notes

Abstract

After avulsion and replantation, teeth are at risk of bone and root resorption. The present study aimed to demonstrate that the intra-nuclear transducible form of transcription modulation domain of p65 (nt-p65-TMD) can suppress osteoclast differentiation in vitro, and reduce bone resorption in a rat model of tooth replantation. Cell viability and nitric oxide release were evaluated in RAW264.7 cells using CCK-8 assay and Griess reaction kit. Osteoclast differentiation was evaluated using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and tartrate-resistant acid phosphatase (TRAP) staining. Thirty-two maxillary rat molars were extracted and stored in saline (n = 10) or 10 µM nt-p65-TMD solution (n = 22) before replantation. After 4 weeks, specimens were scored according to the inflammatory pattern using micro-computed tomography (CT) imaging and histological analyses. nt-p65-TMD treatment resulted in significant reduction of nitric oxide release and osteoclast differentiation as studied using PCR and TRAP staining. Further, micro-CT analysis revealed a significant decrease in bone resorption in the nt-p65-TMD treatment group (p < 0.05). Histological analysis of nt-p65-TMD treatment group showed that not only bone and root resorption, but also inflammation of the periodontal ligament and epithelial insertion was significantly reduced. These findings suggest that nt-p65-TMD has the unique capabilities of regulating bone remodeling after tooth replantation.
Keywords: nuclear factor kappa B; intra-nucleus delivery; osteoclast; tooth replantation nuclear factor kappa B; intra-nucleus delivery; osteoclast; tooth replantation
Graphical Abstract

Share and Cite

MDPI and ACS Style

Kang, C.-M.; Mo, S.; Jeon, M.; Jung, U.-W.; Shin, Y.; Shin, J.-S.; Shin, B.-Y.; Lee, S.-K.; Choi, H.-J.; Song, J.S. Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation. Int. J. Mol. Sci. 2021, 22, 1987. https://doi.org/10.3390/ijms22041987

AMA Style

Kang C-M, Mo S, Jeon M, Jung U-W, Shin Y, Shin J-S, Shin B-Y, Lee S-K, Choi H-J, Song JS. Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation. International Journal of Molecular Sciences. 2021; 22(4):1987. https://doi.org/10.3390/ijms22041987

Chicago/Turabian Style

Kang, Chung-Min, Seunghan Mo, Mijeong Jeon, Ui-Won Jung, Yooseok Shin, Jin-Su Shin, Bo-Young Shin, Sang-Kyou Lee, Hyung-Jun Choi, and Je Seon Song. 2021. "Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation" International Journal of Molecular Sciences 22, no. 4: 1987. https://doi.org/10.3390/ijms22041987

APA Style

Kang, C.-M., Mo, S., Jeon, M., Jung, U.-W., Shin, Y., Shin, J.-S., Shin, B.-Y., Lee, S.-K., Choi, H.-J., & Song, J. S. (2021). Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation. International Journal of Molecular Sciences, 22(4), 1987. https://doi.org/10.3390/ijms22041987

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop