Next Article in Journal
Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study
Next Article in Special Issue
Secretory Production of Functional Grouper Type I Interferon from Epinephelus septemfasciatus in Escherichia coli and Bacillus subtilis
Previous Article in Journal
Developmental Time Course of SNAP-25 Isoforms Regulate Hippocampal Long-Term Synaptic Plasticity and Hippocampus-Dependent Learning
Previous Article in Special Issue
Differential Effects of Dietary Supplementation of Krill Meal, Soybean Meal, Butyrate, and Bactocell® on the Gene Expression of Atlantic Salmon Head Kidney
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 21
Issue 4
10.3390/ijms21041450
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of Aeromonas veronii TH0426

by
Ying Li
1,†,
Bintong Yang
1,2,†,
Jiaxin Tian
1,
Wuwen Sun
1,
Guiqin Wang
1,
Aidong Qian
1,
Chunfeng Wang
1,
Xiaofeng Shan
1,* and
Yuanhuan Kang
1,*
1
Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China
2
College of Life Science, Changchun Sci-Tech University, Changchun 130118, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(4), 1450; https://doi.org/10.3390/ijms21041450
Submission received: 1 January 2020 / Revised: 15 February 2020 / Accepted: 17 February 2020 / Published: 20 February 2020
(This article belongs to the Special Issue Fish Immunology)
Browse Figures
Versions Notes

Abstract

Aeromonas veronii is a virulent fish pathogen that causes extensive economic losses in the aquaculture industry worldwide. In this study, a virulent strain of A. veronii TH0426 was used to establish an in vitro biofilm model. The results show that the biofilm-forming abilities of A. veronii TH0426 were similar in different media, peaking under conditions of 20 °C and pH 6. Further, isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics methods were used to compare the differential expression of A. veronii between the biofilm and planktonic cells. The results show alterations in 277 proteins, with 130 being upregulated and 147 downregulated. Pathway analysis and GO (Gene Ontology) annotations indicated that these proteins are mainly involved in metabolic pathways and the biosynthesis of secondary metabolites and antibiotics. These proteins are the main factors affecting the adaptability of A. veronii to its external environment. MRM (multiple reaction 27 monitoring) and qPCR (qPCR) were used to verify the differential proteins of the selected A. veronii. This is the first report on the biofilm and planktonic cells of A. veronii, thus contributing to studying the infection and pathogenesis of A. veronii.
Keywords: Aeromonas veronii; biofilm; quantitative proteomics; iTRAQ Aeromonas veronii; biofilm; quantitative proteomics; iTRAQ

Share and Cite

MDPI and ACS Style

Li, Y.; Yang, B.; Tian, J.; Sun, W.; Wang, G.; Qian, A.; Wang, C.; Shan, X.; Kang, Y. An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of Aeromonas veronii TH0426. Int. J. Mol. Sci. 2020, 21, 1450. https://doi.org/10.3390/ijms21041450

AMA Style

Li Y, Yang B, Tian J, Sun W, Wang G, Qian A, Wang C, Shan X, Kang Y. An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of Aeromonas veronii TH0426. International Journal of Molecular Sciences. 2020; 21(4):1450. https://doi.org/10.3390/ijms21041450

Chicago/Turabian Style

Li, Ying, Bintong Yang, Jiaxin Tian, Wuwen Sun, Guiqin Wang, Aidong Qian, Chunfeng Wang, Xiaofeng Shan, and Yuanhuan Kang. 2020. "An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of Aeromonas veronii TH0426" International Journal of Molecular Sciences 21, no. 4: 1450. https://doi.org/10.3390/ijms21041450

APA Style

Li, Y., Yang, B., Tian, J., Sun, W., Wang, G., Qian, A., Wang, C., Shan, X., & Kang, Y. (2020). An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of Aeromonas veronii TH0426. International Journal of Molecular Sciences, 21(4), 1450. https://doi.org/10.3390/ijms21041450

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop