Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells

Hanamata, Shigeru; Kurusu, Takamitsu; Kuchitsu, Kazuyuki

doi:10.3390/ijms21239166

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells

Shigeru Hanamata

^1,2,

Takamitsu Kurusu

^1,3,* and

Kazuyuki Kuchitsu

^1,*

Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan

Graduate School of Science and Technology, Niigata University, 2-8050 Ikarashi, Niigata, Nishi-ku 950-2181, Japan

Department of Mechanical and Electrical Engineering, Suwa University of Science, 5000-1 Toyohira, Chino, Nagano 391-0292, Japan

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2020, 21(23), 9166; https://doi.org/10.3390/ijms21239166

Received: 16 November 2020 / Revised: 28 November 2020 / Accepted: 29 November 2020 / Published: 1 December 2020

(This article belongs to the Special Issue New Insight into Signaling and Autophagy in Plants)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Autophagy is ubiquitous in eukaryotic cells and plays an essential role in stress adaptation and development by recycling nutrients and maintaining cellular homeostasis. However, the dynamics and regulatory mechanisms of autophagosome formation during the cell cycle in plant cells remain poorly elucidated. We here analyzed the number of autophagosomes during cell cycle progression in synchronized tobacco BY-2 cells expressing YFP-NtATG8a as a marker for the autophagosomes. Autophagosomes were abundant in the G2 and G1 phases of interphase, though they were much less abundant in the M and S phases. Autophagosomes drastically decreased during the G2/M transition, and the CDK inhibitor roscovitine inhibited the G2/M transition and the decrease in autophagosomes. Autophagosomes were rapidly increased by a proteasome inhibitor, MG-132. MG-132-induced autophagosome formation was also markedly lower in the M phases than during interphase. These results indicate that the activity of autophagosome formation is differently regulated at each cell cycle stage, which is strongly suppressed during mitosis. View Full-Text

Keywords: ATG8; autophagy; cell cycle; tobacco bright yellow-2 (BY-2) cells

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

Supplementary File 1:
ZIP-Document (ZIP, 4386 KiB)

Share and Cite

MDPI and ACS Style

Hanamata, S.; Kurusu, T.; Kuchitsu, K. Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells. Int. J. Mol. Sci. 2020, 21, 9166. https://doi.org/10.3390/ijms21239166

AMA Style

Hanamata S, Kurusu T, Kuchitsu K. Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells. International Journal of Molecular Sciences. 2020; 21(23):9166. https://doi.org/10.3390/ijms21239166

Chicago/Turabian Style

Hanamata, Shigeru, Takamitsu Kurusu, and Kazuyuki Kuchitsu. 2020. "Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells" International Journal of Molecular Sciences 21, no. 23: 9166. https://doi.org/10.3390/ijms21239166

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells

Abstract

Supplementary Material

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI