Dissection of P2X4 and P2X7 Receptor Current Components in BV-2 Microglia
1
Julius-Bernstein-Institute for Physiology, Martin-Luther-University, D-06097 Halle-Wittenberg, Germany
2
Institute of Clinical Pharmacology, RWTH Aachen University, D-52074 Aachen, Germany
3
Pharmaceutical Institute, Pharmaceutical & Mediinal Chemistry, University of Bonn, D-53121 Bonn, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8489; https://doi.org/10.3390/ijms21228489
Received: 23 September 2020
/
Revised: 9 November 2020
/
Accepted: 9 November 2020
/
Published: 11 November 2020
(This article belongs to the Special Issue Molecular, Cellular and Systemic Signature of Microglia)
Microglia cells represent the immune system of the central nervous system. They become activated by ATP released from damaged and inflamed tissue via purinergic receptors. Ionotropic purinergic P2X4 and P2X7 receptors have been shown to be involved in neurological inflammation and pain sensation. Whether the two receptors assemble exclusively as homotrimers or also as heterotrimers is still a matter of debate. We investigated the expression of P2X receptors in BV-2 microglia cells applying the whole-cell voltage-clamp technique. We dissected P2X4 and P2X7 receptor-mediated current components by using specific P2X4 and P2X7 receptor blockers and by their characteristic current kinetics. We found that P2X4 and P2X7 receptors are activated independently from each other, indicating that P2X4/P2X7 heteromers are not of functional significance in these cells. The pro-inflammatory mediators lipopolysaccharide and interferon γ, if applied in combination, upregulated P2X4, but not P2X7 receptor-dependent current components also arguing against phenotypically relevant heteromerization of P2X4 and P2X7 receptor subunits.
View Full-Text
Keywords:
P2 purinergic receptor; P2X7 receptor; P2X4 receptor; voltage clamp; microglia
▼
Show Figures
Graphical abstract
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Supplementary File 1:
PDF-Document (PDF, 380 KiB)
MDPI and ACS Style
Trang, M.; Schmalzing, G.; Müller, C.E.; Markwardt, F. Dissection of P2X4 and P2X7 Receptor Current Components in BV-2 Microglia. Int. J. Mol. Sci. 2020, 21, 8489. https://doi.org/10.3390/ijms21228489
AMA Style
Trang M, Schmalzing G, Müller CE, Markwardt F. Dissection of P2X4 and P2X7 Receptor Current Components in BV-2 Microglia. International Journal of Molecular Sciences. 2020; 21(22):8489. https://doi.org/10.3390/ijms21228489
Chicago/Turabian StyleTrang, Mira, Günther Schmalzing, Christa E. Müller, and Fritz Markwardt. 2020. "Dissection of P2X4 and P2X7 Receptor Current Components in BV-2 Microglia" International Journal of Molecular Sciences 21, no. 22: 8489. https://doi.org/10.3390/ijms21228489
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
