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Article

De Novo Assembly of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Psyllidae) Transcriptome across Developmental Stages

by
Chunxiao Yang
1,2,3,
Da Ou
2,
Wei Guo
2,
Jing Lü
2,
Changfei Guo
2,
Baoli Qiu
2,* and
Huipeng Pan
2,*
1
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China
2
Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Guangzhou 510642, China
3
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(14), 4974; https://doi.org/10.3390/ijms21144974
Submission received: 21 May 2020 / Revised: 9 July 2020 / Accepted: 12 July 2020 / Published: 14 July 2020
(This article belongs to the Section Molecular Biology)
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Abstract

Asian citrus psyllid Diaphorina citri Kuwayama is an important economic pest of citrus, as it transmits Candidatus Liberibacter asiaticus, the causative agent of huanglongbing. In this study, we used RNA-seq to identify novel genes and provide the first high-resolution view of the of D. citri transcriptome throughout development. The transcriptomes of D. citri during eight developmental stages, including the egg, five instars, and male and female adults were sequenced. In total, 115 million clean reads were obtained and assembled into 354,726 unigenes with an average length of 925.65 bp and an N50 length of 1733 bp. Clusters of Orthologous Groups, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to functionally annotate the genes. Differential expression analysis highlighted developmental stage-specific expression patterns. Furthermore, two trehalase genes were characterized with lower expression in adults compared to that in the other stages. The RNA interference (RNAi)-mediated suppression of the two trehalase genes resulted in significantly high D. citri mortality. This study enriched the genomic information regarding D. citri. Importantly, these data represent the most comprehensive transcriptomic resource currently available for D. citri and will facilitate functional genomics studies of this notorious pest.
Keywords: Diaphorina citri; transcriptome annotation; developmental stage; differential expression; trehalase Diaphorina citri; transcriptome annotation; developmental stage; differential expression; trehalase

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MDPI and ACS Style

Yang, C.; Ou, D.; Guo, W.; Lü, J.; Guo, C.; Qiu, B.; Pan, H. De Novo Assembly of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Psyllidae) Transcriptome across Developmental Stages. Int. J. Mol. Sci. 2020, 21, 4974. https://doi.org/10.3390/ijms21144974

AMA Style

Yang C, Ou D, Guo W, Lü J, Guo C, Qiu B, Pan H. De Novo Assembly of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Psyllidae) Transcriptome across Developmental Stages. International Journal of Molecular Sciences. 2020; 21(14):4974. https://doi.org/10.3390/ijms21144974

Chicago/Turabian Style

Yang, Chunxiao, Da Ou, Wei Guo, Jing Lü, Changfei Guo, Baoli Qiu, and Huipeng Pan. 2020. "De Novo Assembly of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Psyllidae) Transcriptome across Developmental Stages" International Journal of Molecular Sciences 21, no. 14: 4974. https://doi.org/10.3390/ijms21144974

APA Style

Yang, C., Ou, D., Guo, W., Lü, J., Guo, C., Qiu, B., & Pan, H. (2020). De Novo Assembly of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Psyllidae) Transcriptome across Developmental Stages. International Journal of Molecular Sciences, 21(14), 4974. https://doi.org/10.3390/ijms21144974

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