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Correction: Zhang, Z.; et al. Roles of MicroRNAs in Establishing and Modulating Stem Cell Potential. Int. J. Mol. Sci. 2019, 20, 3643
 
 
Article

Consolidated BRCA1/2 Variant Interpretation by MH BRCA Correlates with Predicted PARP Inhibitor Efficacy Association by MH Guide

1
Genome Analysis Center, Yamanashi Central Hospital, Kofu 400-8506, Japan
2
Molecular Health GmbH, Research Department, 69115 Heidelberg, Germany
3
Department of Breast Surgery, Yamanashi Central Hospital, Kofu 400-8506, Japan
4
Department of Obstetrics and Gynecology, Yamanashi Central Hospital, Kofu 400-8506, Japan
5
University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(11), 3895; https://doi.org/10.3390/ijms21113895
Received: 30 March 2020 / Revised: 20 May 2020 / Accepted: 29 May 2020 / Published: 29 May 2020
(This article belongs to the Section Molecular Genetics and Genomics)
BRCA1/2 variants are prognostic biomarkers for hereditary breast and/or ovarian cancer (HBOC) syndrome and predictive biomarkers for PARP inhibition. In this study, we benchmarked the classification of BRCA1/2 variants from patients with HBOC-related cancer using MH BRCA, a novel computational technology that combines the ACMG guidelines with expert-curated variant annotations. Evaluation of BRCA1/2 variants (n = 1040) taken from four HBOC studies showed strong concordance within the pathogenic (98.1%) subset. Comparison of MH BRCA’s ACMG classification to ClinVar submitter content from ENIGMA, the international consortium of investigators on the clinical significance of BRCA1/2 variants, the ARUP laboratories, a clinical testing lab of the University of UTAH, and the German Cancer Consortium showed 99.98% concordance (4975 out of 4976 variants) in the pathogenic subset. In our patient cohort, refinement of patients with variants of unknown significance reduced the uncertainty of cancer-predisposing syndromes by 64.7% and identified three cases with potential family risk to HBOC due to a likely pathogenic variant BRCA1 p.V1653L (NM_007294.3:c.4957G > T; rs80357261). To assess whether classification results predict PARP inhibitor efficacy, contextualization with functional impact information on DNA repair activity were performed, using MH Guide. We found a strong correlation between treatment efficacy association and MH BRCA classifications. Importantly, low efficacy to PARP inhibition was predicted in 3.95% of pathogenic variants from four examined HBOC studies and our patient cohort, indicating the clinical relevance of the consolidated variant interpretation. View Full-Text
Keywords: BRCA1; BRCA2; PARP inhibition; HBOC; cancer BRCA1; BRCA2; PARP inhibition; HBOC; cancer
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MDPI and ACS Style

Hirotsu, Y.; Schmidt-Edelkraut, U.; Nakagomi, H.; Sakamoto, I.; Hartenfeller, M.; Narang, R.; Soldatos, T.G.; Kaduthanam, S.; Wang, X.; Hettich, S.; Brock, S.; Jackson, D.B.; Omata, M. Consolidated BRCA1/2 Variant Interpretation by MH BRCA Correlates with Predicted PARP Inhibitor Efficacy Association by MH Guide. Int. J. Mol. Sci. 2020, 21, 3895. https://doi.org/10.3390/ijms21113895

AMA Style

Hirotsu Y, Schmidt-Edelkraut U, Nakagomi H, Sakamoto I, Hartenfeller M, Narang R, Soldatos TG, Kaduthanam S, Wang X, Hettich S, Brock S, Jackson DB, Omata M. Consolidated BRCA1/2 Variant Interpretation by MH BRCA Correlates with Predicted PARP Inhibitor Efficacy Association by MH Guide. International Journal of Molecular Sciences. 2020; 21(11):3895. https://doi.org/10.3390/ijms21113895

Chicago/Turabian Style

Hirotsu, Yosuke, Udo Schmidt-Edelkraut, Hiroshi Nakagomi, Ikuko Sakamoto, Markus Hartenfeller, Ram Narang, Theodoros G. Soldatos, Sajo Kaduthanam, Xiaoyue Wang, Stephan Hettich, Stephan Brock, David B. Jackson, and Masao Omata. 2020. "Consolidated BRCA1/2 Variant Interpretation by MH BRCA Correlates with Predicted PARP Inhibitor Efficacy Association by MH Guide" International Journal of Molecular Sciences 21, no. 11: 3895. https://doi.org/10.3390/ijms21113895

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