Resveratrol Promotes Mitochondrial Biogenesis and Protects against Seizure-Induced Neuronal Cell Damage in the Hippocampus Following Status Epilepticus by Activation of the PGC-1α Signaling Pathway
by
Yao-Chung Chuang 1,2,3,4,5,†, Shang-Der Chen 1,2,3,†
, Chung-Yao Hsu 4, Shu-Fang Chen 1, Nai-Ching Chen 1 and Shuo-Bin Jou 6,*
Yao-Chung Chuang 1,2,3,4,5,†, Shang-Der Chen 1,2,3,†, Chung-Yao Hsu 4, Shu-Fang Chen 1, Nai-Ching Chen 1 and Shuo-Bin Jou 6,*
1
Department of Neurology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung City 83301, Taiwan
2
Institute for Translation Research in Biomedicine; Kaohsiung Chang Gung Memorial Hospital, Kaohsiung City 83301, Taiwan
3
College of Medicine, Chang Gung University, Taoyuan City 33302, Taiwan
4
Department of Neurology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan
5
Department of Biological Science, National Sun Yat-sen University, Kaohsiung City 80424, Taiwan
6
Department of Neurology, Mackay Memorial Hospital and Mackay Medical College, Taipei 252, Taiwan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(4), 998; https://doi.org/10.3390/ijms20040998
Received: 22 January 2019 / Revised: 13 February 2019 / Accepted: 21 February 2019 / Published: 25 February 2019
(This article belongs to the Special Issue Health Benefits of Resveratrol)
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is known to regulate mitochondrial biogenesis. Resveratrol is present in a variety of plants, including the skin of grapes, blueberries, raspberries, mulberries, and peanuts. It has been shown to offer protective effects against a number of cardiovascular and neurodegenerative diseases, stroke, and epilepsy. This study examined the neuroprotective effect of resveratrol on mitochondrial biogenesis in the hippocampus following experimental status epilepticus. Kainic acid was microinjected into left hippocampal CA3 in Sprague Dawley rats to induce bilateral prolonged seizure activity. PGC-1α expression and related mitochondrial biogenesis were investigated. Amounts of nuclear respiratory factor 1 (NRF1), mitochondrial transcription factor A (Tfam), cytochrome c oxidase 1 (COX1), and mitochondrial DNA (mtDNA) were measured to evaluate the extent of mitochondrial biogenesis. Increased PGC-1α and mitochondrial biogenesis machinery after prolonged seizure were found in CA3. Resveratrol increased expression of PGC-1α, NRF1, and Tfam, NRF1 binding activity, COX1 level, and mtDNA amount. In addition, resveratrol reduced activated caspase-3 activity and attenuated neuronal cell damage in the hippocampus following status epilepticus. These results suggest that resveratrol plays a pivotal role in the mitochondrial biogenesis machinery that may provide a protective mechanism counteracting seizure-induced neuronal damage by activation of the PGC-1α signaling pathway.
View Full-Text
Keywords:
resveratrol; PGC-1α; mitochondrial biogenesis; status epilepticus; hippocampus
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Chuang, Y.-C.; Chen, S.-D.; Hsu, C.-Y.; Chen, S.-F.; Chen, N.-C.; Jou, S.-B. Resveratrol Promotes Mitochondrial Biogenesis and Protects against Seizure-Induced Neuronal Cell Damage in the Hippocampus Following Status Epilepticus by Activation of the PGC-1α Signaling Pathway. Int. J. Mol. Sci. 2019, 20, 998.
Show more citation formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
