Inverse Regulation of Lipocalin-2/24p3 Receptor/SLC22A17 and Lipocalin-2 Expression by Tonicity, NFAT5/TonEBP and Arginine Vasopressin in Mouse Cortical Collecting Duct Cells mCCD(cl.1): Implications for Osmotolerance
Abstract
:1. Introduction
2. Results
2.1. Hyperosmolarity Increases the Expression of Slc22a17/SLC22A17 in mCCD(cl.1) Cells
2.2. Hypertonicity Dependent Upregulation of Slc22a17/SLC22A17 and Downregulation of Lcn2/LCN2 are Mediated by NFAT5 in mCCD(cl.1) Cells
2.3. ddAVP Upregulates Slc22a17/SLC22A17 via cAMP/CREB Signaling in mCCD(cl.1) Cells
2.4. LPS Downregulates Slc22a17/SLC22A17 and Upregulates Lcn2/LCN2 in mCCD(cl.1) Cells
2.5. ddAVP Posttranslationally Downregulates Unstimulated and LPS-Stimulated LCN2 in mCCD(cl.1) Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Methods
4.2.1. Culture of mCCD(cl.1) Cells
4.2.2. Osmolarity/Tonicity Experiments
4.2.3. Transient Transfection
4.2.4. RNA Extraction, cDNA Synthesis and Reverse Transcription PCR (RT-PCR)
4.2.5. Quantitative PCR (qPCR)
4.2.6. Measurements of Transepithelial Electrical Resistance (TEER) of Cell Monolayers
4.2.7. Determination of LCN2 Expression and Secretion
4.2.8. Isolation of Plasma Membrane Enriched Microsomes
4.2.9. Isolation of Nuclei
4.2.10. Immunoblotting
4.2.11. Immunofluorescence Staining and Microscopy
4.2.12. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Immunogen | Host Species | Manufacturer | Catalog # | Application | Dilution |
---|---|---|---|---|---|
Rat LCN2 | goat | RD Biosystems | AF3508 | IB * | 1:200–1:300 |
Mouse LCN2 | rabbit | Abcam | Ab63929 | IF * | 1:250–1:1000 |
Rat SLC22A17 (C-terminus) | rabbit | [14] | Ig-1086 | IB | 1:100–1:2000 |
Rat SLC22A17 (N-terminus) | rabbit | [14] | Ig-1095 | IF | 1:100–1:1000 |
Human b-actin | mouse | [14] | A5316 | IB | 1:20,000 |
Human GAPDH (14C10) | mouse | Sigma-Aldrich | 2118 | IB | 1:20,000 |
Human Na+,K+- ATPase a1-subunit | rabbit | Cell Signaling | 3010S | IB | 1:250–1:500 |
Human Lamin A/C (4C11) | mouse | Cell Signaling | 4777 | IB | 1:20,000 |
Human NFAT5 | rabbit | [24] | N/A | IB | 1:6000 |
Human IkB-a | rabbit | Santa Cruz | Sc-371 | IB | 1:1000 |
Gapdh | Aqp2 | Lcn2 | Slc22a17 | |
---|---|---|---|---|
Accession number | NM_001289726.1 | NM_009699.3 | NM_008491.1 | NM_021551.4 |
Forward primer (5′-3′) | AGGGCTCATGACCACAGT | TGGCTGTCAATGCTCTCCAC | CCACCACGGACTACAACCAG | CAGCCACCTCCTAACCGCTGTG |
Reverse primer (5′-3′) | TGCAGGGATGATGTTCTG | GGAGCAGCCGGTGAAATAGA | AGCTCCTTGGTTCTTCCATACA | CTCCCACTAGGCTCAAAGGCTGCT |
Reference | NCBI Primer-BLAST | [53] | [39] | [39] |
Activation | 5 min 95 °C | 5 min 95 °C | 5 min 95 °C | 5 min 95 °C |
Cycle number | 18–22 | 31–33 | 20–25 | 27–29 |
Denaturation | 30 s 94 °C | 30 s 94°C | 30 s 94 °C | 30 s 94 °C |
Annealing | 30 s 60 °C | 30 s 62 °C | 30 s 60 °C | 30 s 60 °C |
Extension | 30 s 72 °C | 30 s 72 °C | 30 s 72 °C | 30 s 72 °C |
Final Extension | 7 min 72 °C | 7 min 72 °C | 7 min 72 °C | 7 min 72 °C |
PCR product (bp) | 112 | 200 | 100 | 86 |
Gene-Name (Accession Number) | Forward (5′-3′) | Reverse (5′-3′) | Reference | Amplicon Size (bp) | Efficiency(%) |
---|---|---|---|---|---|
Actb (NM_007393.5) | CGTGCGTGACATCAAAGAGAA | GGCCATCTC CTGCTCGAA | [20] | 102 | 102 |
Gapdh (NM_001289726.1) | CGGCCGCATCTTCTTGTG | CCGACCTTCACCATTTTGTCTAC | [20] | 100 | 100 |
Lcn2 (NM_008491.1) | CCACCACGGACTACAACCAG | AGCTCCTTGGTTCTTCCATACA | [20] | 98 | 98 |
Slc22a17 (NM_021551.4) | CAGCCACCTCCTAACCGCTGTG | CTCCCACTAGGCTCAAAGGCTGCT | [20] | 110 | 110 |
Aqp2 (NM_009699.3) | TGGCTGTCAATGCTCTCCAC | GGAGCAGCCGGTGAAATAGA | [53] | 92 | 92 |
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Probst, S.; Scharner, B.; McErlean, R.; Lee, W.-K.; Thévenod, F. Inverse Regulation of Lipocalin-2/24p3 Receptor/SLC22A17 and Lipocalin-2 Expression by Tonicity, NFAT5/TonEBP and Arginine Vasopressin in Mouse Cortical Collecting Duct Cells mCCD(cl.1): Implications for Osmotolerance. Int. J. Mol. Sci. 2019, 20, 5398. https://doi.org/10.3390/ijms20215398
Probst S, Scharner B, McErlean R, Lee W-K, Thévenod F. Inverse Regulation of Lipocalin-2/24p3 Receptor/SLC22A17 and Lipocalin-2 Expression by Tonicity, NFAT5/TonEBP and Arginine Vasopressin in Mouse Cortical Collecting Duct Cells mCCD(cl.1): Implications for Osmotolerance. International Journal of Molecular Sciences. 2019; 20(21):5398. https://doi.org/10.3390/ijms20215398
Chicago/Turabian StyleProbst, Stephanie, Bettina Scharner, Ruairi McErlean, Wing-Kee Lee, and Frank Thévenod. 2019. "Inverse Regulation of Lipocalin-2/24p3 Receptor/SLC22A17 and Lipocalin-2 Expression by Tonicity, NFAT5/TonEBP and Arginine Vasopressin in Mouse Cortical Collecting Duct Cells mCCD(cl.1): Implications for Osmotolerance" International Journal of Molecular Sciences 20, no. 21: 5398. https://doi.org/10.3390/ijms20215398