Next Article in Journal
Apoferritin Modified Magnetic Particles as Doxorubicin Carriers for Anticancer Drug Delivery
Previous Article in Journal
Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2013, 14(7), 13377-13390;

A Labile Pool of IQGAP1 Disassembles Endothelial Adherens Junctions

Department of Anesthesiology, 1st Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, China
School of Bioscience & Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, Guangdong, China
Nanotides Inc., 401 Professional Drive, Suite 130, Gaithersburg, MD 20879, USA
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 10 April 2013 / Revised: 20 June 2013 / Accepted: 21 June 2013 / Published: 27 June 2013
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [1712 KB, uploaded 19 June 2014]   |  


Adhesion molecules are known to play an important role in endothelial activation and angiogenesis. Here we determined the functional role of IQGAP1 in the regulation of endothelial adherens junctions. VE-cadherin is found to be associated with actin filaments and thus stable, but IQGAP1 at intercellular junctions is not bound to actin filaments and thus labile. Expression of GFP labeled VE-α-catenin is shown to increase the electrical resistance across HUVEC monolayers and diminishes endogenous labile IQGAP1 at the intercellular junctions. Knockdown of endogenous IQGAP1 enhances intercellular adhesion in HUVECs by increasing the association of VE-cadherin with P120 and β-catenin. IQGAP1 knockdown also decreases the interaction of N-cadherin with P120 and β-catenin. Together, these results suggest that a labile pool of IQGAP1 at intercellular junctions disassembles adherens junctions and thus impairs endothelial cell-cell adhesion. View Full-Text
Keywords: IQGAP1; Endothelial Adherens Junction; VE-cadherin; N-cadherin IQGAP1; Endothelial Adherens Junction; VE-cadherin; N-cadherin

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Yuan, Z.; Zhang, W.; Tan, W. A Labile Pool of IQGAP1 Disassembles Endothelial Adherens Junctions. Int. J. Mol. Sci. 2013, 14, 13377-13390.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top