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Review

Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies

1
Center for Biomolecular Structure and Organization, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA
2
Vertex Pharmaceuticals, 50 Northern Avenue, Boston, MA 02210, USA
*
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Academic Editor: Esteban Alonso
Molecules 2021, 26(18), 5581; https://doi.org/10.3390/molecules26185581
Received: 25 July 2021 / Revised: 5 September 2021 / Accepted: 7 September 2021 / Published: 14 September 2021
(This article belongs to the Special Issue Nucleosides and Nucleotides)
RNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution. Still, RNA analysis by NMR suffers from spectral overlap and line broadening, both of which worsen for larger RNAs. Incorporation of stable isotope labels into RNA has provided several solutions to these challenges. In this review, we summarize the benefits and limitations of various methods used to obtain isotope-labeled RNA building blocks and how they are used to prepare isotope-labeled RNA for NMR structure and dynamics studies. View Full-Text
Keywords: stable isotope; enzymes; nucleotides; NMR spectroscopy; RNA; structure; dynamics stable isotope; enzymes; nucleotides; NMR spectroscopy; RNA; structure; dynamics
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MDPI and ACS Style

Olenginski, L.T.; Taiwo, K.M.; LeBlanc, R.M.; Dayie, T.K. Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies. Molecules 2021, 26, 5581. https://doi.org/10.3390/molecules26185581

AMA Style

Olenginski LT, Taiwo KM, LeBlanc RM, Dayie TK. Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies. Molecules. 2021; 26(18):5581. https://doi.org/10.3390/molecules26185581

Chicago/Turabian Style

Olenginski, Lukasz T., Kehinde M. Taiwo, Regan M. LeBlanc, and Theodore K. Dayie 2021. "Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies" Molecules 26, no. 18: 5581. https://doi.org/10.3390/molecules26185581

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