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Molecules 2018, 23(4), 958; https://doi.org/10.3390/molecules23040958

Anti-Diabetic Effects of Acankoreagenin from the Leaves of Acanthopanax Gracilistylus Herb in RIN-m5F Cells via Suppression of NF-κB Activation

1
School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
2
School of Life Sciences, Datong University, Datong 037009, China
3
Changsha Broad-Ocean Bio-Science and Technique Co., Ltd., Changsha 410205, China
4
Department of Food and Nutrition, Wonkwang University, Iksan 54538, Korea
These authors contribute equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 27 March 2018 / Revised: 13 April 2018 / Accepted: 16 April 2018 / Published: 19 April 2018
(This article belongs to the Special Issue Medicinal Plants and Diabetes)
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Abstract

Diabetes mellitus is a chronic degenerative disease that causes long-term complications and represents a serious public health problem. In this manuscript, acankoreagenin isolated from the leaves of Acanthopanax gracilistylus (LAG) is thought to possess excellent anti-diabetic properties. In vitro, anti-diabetic activities were assessed based on the inhibitory activities with α-glucosidase (IC50 13.01 μM), α-amylase (IC50 30.81 μM), and PTP1B (IC50 16.39 μM). Acankoreagenin showed better anti-diabetic effects. Then, an investigation was performed to analyze the insulin secretion effects of the insulin-secreting cell line in RIN-m5F cells. It was found that acankoreagenin could increase the insulin release in RIN-m5F cells. It was also found that acankoreagenin reduced NO production, activity of caspase-3, and the reactive oxygen species levels in the cells injured by processing of cytokines. In western blotting, inactivation of NF-κB signaling was confirmed. Acankoreagenin (20 μM) showed a higher I-κBα expression and lower NF-κB expression than the control group and showed a better expression than the positive control L-NAME (1 mM) (p < 0.05). This study demonstrates the anti-diabetic effects of acankoreagenin in vitro and suggests acankoreagenin might offer therapeutic potential for treating diabetes mellitus. View Full-Text
Keywords: Acanthopanax gracilistylus; acankoreagenin; anti-diabetic effects; α-glucosidase; α-amylase; PTP1B; RIN-m5F cells Acanthopanax gracilistylus; acankoreagenin; anti-diabetic effects; α-glucosidase; α-amylase; PTP1B; RIN-m5F cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Lu, M.-X.; Yang, Y.; Zou, Q.-P.; Luo, J.; Zhang, B.-B.; Liu, X.-Q.; Hwang, E.-H. Anti-Diabetic Effects of Acankoreagenin from the Leaves of Acanthopanax Gracilistylus Herb in RIN-m5F Cells via Suppression of NF-κB Activation. Molecules 2018, 23, 958.

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