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Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment

1
Institute of Nutrition and food Safety, Xiamen Medical College, Xiamen 361023, China
2
College of Food Science, South China Agricultural University, Guangzhou 510642, China
3
Department of Biology and Chemistry Engineering, Fuqing Branch of Fujian Normal University, Fuzhou 50300, China
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(3), 570; https://doi.org/10.3390/molecules23030570
Received: 3 February 2018 / Revised: 22 February 2018 / Accepted: 28 February 2018 / Published: 3 March 2018
The pulsed electric fields (PEF) treatment is a novel method for obtaining glycated proteins by way of a Maillard reaction between proteins and polysaccharides but its effect on the preparation of protein–monosaccharide conjugate has not been explored. This study aimed to prepare bovine serum albumin (BSA)–glucose and BSA–mannose conjugates using PEF in pH 10.0 at an intensity of 10 or 20 kV/cm, frequency of 1 kHz, pulse width of 20 μs and 73.5 pulses. The conjugates were evaluated for physicochemical properties. The results indicated that PEF not only promoted Maillard reaction between BSA and glucose or mannose but also alleviated the undesirable browning. PEF treatment favored the increased surface hydrophobicity and emulsifying activity in BSA but reduced surface hydrophobicity and foaming stability and improved foaming capacity in BSA–glucose and BSA–mannose conjugates. These findings provided useful considerations in the application of PEF treatment as a potential method to prepare BSA–monosaccharide conjugates by Maillard reaction. View Full-Text
Keywords: bovine serum albumin; glucose; mannose; physicochemical properties; pulsed electric fields treatment bovine serum albumin; glucose; mannose; physicochemical properties; pulsed electric fields treatment
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Jian, W.; Wang, L.; Wu, L.; Sun, Y.-M. Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment. Molecules 2018, 23, 570.

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