Next Article in Journal
A New Cycloartane-Type Triterpenoid Saponin Xanthine Oxidase Inhibitor from Homonoia riparia Lour
Next Article in Special Issue
Study of Leaf Metabolome Modifications Induced by UV-C Radiations in Representative Vitis, Cissus and Cannabis Species by LC-MS Based Metabolomics and Antioxidant Assays
Previous Article in Journal
Effect of Apitherapy Formulations against Carbon Tetrachloride-Induced Toxicity in Wistar Rats after Three Weeks of Treatment
Previous Article in Special Issue
Effectiveness of Phenolic Compounds against Citrus Green Mould
Open AccessArticle

Effects of Endogenous Signals and Fusarium oxysporum on the Mechanism Regulating Genistein Synthesis and Accumulation in Yellow Lupine and Their Impact on Plant Cell Cytoskeleton

1
Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, Poznań 60-637, Poland
2
Laboratory of Electron and Confocal Microscopy, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, Poznań 60-614, Poland
3
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Z. Noskowskiego 12/14, Poznań 61-704, Poland
4
Laboratory of Molecular Biology Techniques, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, Poznań 60-614, Poland
5
Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Dojazd 11, Poznań 60-632, Poland
6
Institute of Molecular Physics, Polish Academy of Sciences, Smoluchowskiego 17, Poznań 60-179, Poland
7
Department of Molecular and Cellular Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, Poznań 60-614, Poland
*
Author to whom correspondence should be addressed.
Molecules 2014, 19(9), 13392-13421; https://doi.org/10.3390/molecules190913392
Received: 26 April 2014 / Revised: 7 August 2014 / Accepted: 18 August 2014 / Published: 29 August 2014
(This article belongs to the Special Issue Phytoalexins: Current Progress and Future Prospects)
The aim of the study was to examine cross-talk interactions of soluble sugars (sucrose, glucose and fructose) and infection caused by Fusarium oxysporum f.sp. lupini on the synthesis of genistein in embryo axes of Lupinus luteus L.cv. Juno. Genistein is a free aglycone, highly reactive and with the potential to inhibit fungal infection and development of plant diseases. As signal molecules, sugars strongly stimulated accumulation of isoflavones, including genistein, and the expression of the isoflavonoid biosynthetic genes. Infection significantly enhanced the synthesis of genistein and other isoflavone aglycones in cells of embryo axes of yellow lupine with high endogenous sugar levels. The activity of β-glucosidase, the enzyme that releases free aglycones from their glucoside bindings, was higher in the infected tissues than in the control ones. At the same time, a very strong generation of the superoxide anion radical was observed in tissues with high sugar contents already in the initial stage of infection. During later stages after inoculation, a strong generation of semiquinone radicals was observed, which level was relatively higher in tissues deficient in sugars than in those with high sugar levels. Observations of actin and tubulin cytoskeletons in cells of infected embryo axes cultured on the medium with sucrose, as well as the medium without sugar, showed significant differences in their organization. View Full-Text
Keywords: genistein; sugars; actin and tubulin cytoskeletons; free radicals; Lupinus luteus; Fusarium oxysporum genistein; sugars; actin and tubulin cytoskeletons; free radicals; Lupinus luteus; Fusarium oxysporum
Show Figures

Figure 1

MDPI and ACS Style

Formela, M.; Samardakiewicz, S.; Marczak, Ł.; Nowak, W.; Narożna, D.; Bednarski, W.; Kasprowicz-Maluśki, A.; Morkunas, I. Effects of Endogenous Signals and Fusarium oxysporum on the Mechanism Regulating Genistein Synthesis and Accumulation in Yellow Lupine and Their Impact on Plant Cell Cytoskeleton. Molecules 2014, 19, 13392-13421.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop