Next Article in Journal
Synthesis of Pregnane Derivatives, Their Cytotoxicity on LNCap and PC-3 Cells, and Screening on 5α-Reductase Inhibitory Activity
Previous Article in Journal
3-(2-Aminophenyl)-4-methyl-1,3-thiazole-2(3H)-thione as an Ecofriendly Sulphur Transfer Agent to Prepare Alkanethiols in High Yield and High Purity
Article Menu

Export Article

Open AccessArticle
Molecules 2009, 14(11), 4644-4654;

High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity

UNESP-Univ Estadual Paulista, Instituto de Química, Departamento de Química Orgânica, CP 355, CEP 14800-900, Araraquara, São Paulo, Brazil
UEMS, Course of Chemistry, Mato Grosso do Sul State University, CP 351, CEP 79804-970, Dourados, Mato Grosso do Sul, Brazil
Author to whom correspondence should be addressed.
Received: 9 October 2009 / Revised: 13 November 2009 / Accepted: 16 November 2009 / Published: 16 November 2009
Full-Text   |   PDF [163 KB, uploaded 18 June 2014]   |  


Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines. View Full-Text
Keywords: eriocaulaceae; liquid chromatography; quantification; flavonoids eriocaulaceae; liquid chromatography; quantification; flavonoids

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Da Silva, M.A.; Cardoso, C.A.L.; Vilegas, W.; Dos Santos, L.C. High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity. Molecules 2009, 14, 4644-4654.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top