Search Results (547)

Pardosa pseudoannulata plays an important role in the biological control of insect pests. The inclusion of yeast in the culture medium is very important for the growth, development, and reproduction of Drosophila melanogaster, but there have been few studies on the influence of nutrients in the culture medium on spider development. In order to explore the effects of different yeast treatments on the growth and development of D. melanogaster and as a predator, P.  pseudoannulata, three treatments (no yeast, active yeast added, and inactivated yeast added) were adopted to modify the conventional D. melanogaster culture medium. The addition of yeast to the medium shortened the development time from larva to pupation in D. melanogaster. The emergence and larval developmental times of D. melanogaster reared with activated yeast were shorter than those of the group without yeast addition, which promoted D. melanogaster emergence and increased body weight. The addition of yeast to the medium increased the fat, protein, and glucose content in D. melanogaster. The addition of activated yeast shortened the developmental time of P.  pseudoannulata at the second instar stage but had no effect on other instars. Different yeast treat-ments in the medium had no effect on the body length or body weight of P.  pseudoannulata. Adding yeast to D. melanogaster culture medium can increase the total fat content in P.  pseudoannulata, but it has no effect on glucose and total protein in P.  pseudoannulata. Our study shows the importance of yeast to the growth and development of fruit flies. Full article

Background/Objectives: Global warming and rising CO₂ concentrations pose significant challenges to plant systems. Amid these pressures, this study contributes to understanding how tropical species respond by simultaneously evaluating reproductive and genetic traits. It specifically investigates the effects of maternal exposure to warming and elevated CO₂ on progeny physiology, genetic diversity, and population structure in Stylosanthes capitata, a resilient forage legume native to Brazil. Methods: Maternal plants were cultivated under controlled treatments, including ambient conditions (control), elevated CO₂ at 600 ppm (eCO₂), elevated temperature at +2 °C (eTE), and their combined exposure (eTEeCO₂), within a Trop-T-FACE field facility (Temperature Free-Air Controlled Enhancement and Free-Air Carbon Dioxide Enrichment). Seed traits (seeds per inflorescence, hundred-seed mass, abortion, non-viable seeds, coat color, germination at 32, 40, 71 weeks) and abnormal seedling rates were quantified. Genetic diversity metrics included the average (A) and effective (Ae) number of alleles, observed (Ho) and expected (He) heterozygosity, and inbreeding coefficient (Fis). Population structure was assessed using Principal Coordinates Analysis (PCoA), Analysis of Molecular Variance (AMOVA), number of migrants per generation (Nm), and genetic differentiation index (Fst). Two- and three-way Analysis of Variance (ANOVA) were used to evaluate factor effects. Results: Compared to control conditions, warming increased seeds per inflorescence (+46%), reduced abortion (−42.9%), non-viable seeds (−57%), and altered coat color. The germination speed index (GSI +23.5%) and germination rate (Gr +11%) improved with warming; combined treatments decreased germination time (GT −9.6%). Storage preserved germination traits, with warming enhancing performance over time and reducing abnormal seedlings (−54.5%). Conversely, elevated CO₂ shortened GSI in late stages, impairing germination efficiency. Warming reduced Ae (−35%), He (−20%), and raised Fis (maternal 0.50, progeny 0.58), consistent with the species’ mixed mating system; A and Ho were unaffected. Allele frequency shifts suggested selective pressure under eTE. Warming induced slight structure in PCoA, and AMOVA detected 1% (maternal) and 9% (progeny) variation. Fst = 0.06 and Nm = 3.8 imply environmental influence without isolation. Conclusions: Warming significantly shapes seed quality, reproductive success, and genetic diversity in S. capitata. Improved reproduction and germination suggest adaptive advantages, but higher inbreeding and reduced diversity may constrain long-term resilience. The findings underscore the need for genetic monitoring and broader genetic bases in cultivars confronting environmental stressors. Full article

In order to obtain the optimal heat treatment process of Er-containing 7075 aluminum alloy, the effects of pre-stretching and the interval time between pre-stretching and aging on the microstructure and mechanical properties of Er-containing 7075 aluminum alloy during solution treatment followed by pre-stretching and two-stage aging processes were investigated by mechanical property tests, metallographic tests, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The results show that the mechanical properties of Er-containing 7075 aluminum alloy can be significantly improved by increasing the extrusion ratio. Pre-stretching provides nucleation sites for the precipitation of reinforcing phases, accelerates the aging strengthening process, and shortens the peak aging time. The crack source of fracture in Er-containing 7075 aluminum alloy is attributed to the segregated second phases containing Cu and Er in the alloy. The research results have significant engineering significance for the optimization of the heat treatment process of Er-containing 7075 aluminum alloy. Full article

Hot work die steel is an alloy steel with good high-temperature performance, which is widely used in mechanical manufacturing, aerospace, and other fields. During the working process of hot working mold steel, it is subjected to high temperature, wear, and other effects, which can lead to a decrease in the surface hardness of the mold, accelerate surface damage, shorten the service life, and reduce the quality of the workpiece. In order to improve the wear resistance of the mold, this paper conducts two surface treatments, chrome plating and nitriding, on the surface of hot work mold steel, and compares the high-temperature wear behavior of the materials after the two surface treatments. The results indicate that the hot work die steel obtained higher surface hardness and wear resistance after nitriding surface modification. After nitriding treatment, the surface of hot work die steel contains ε phase (Fe_2–3N), which improves its surface hardness and wear resistance, thus exhibiting better surface hardness and wear resistance than the chrome-plated sample. In this study, the high-temperature wear behavior of hot work die steel after two kinds of surface strengthening treatments was deeply discussed, and the high-temperature wear mechanism of steel after surface strengthening was revealed. It provides a theoretical basis and experimental basis for the surface modification of hot working die steel, and also provides new ideas and methods for improving the service life and workpiece quality of hot working die steel in industrial production. In this study, the advantages and disadvantages of high-temperature wear resistance of hot working die steel after chromium plating and nitriding were systematically compared for the first time, which provided a scientific basis for the selection of surface strengthening technology of hot working die steel and had important academic value and practical application significance. Full article

Malondialdehyde (MDA), a mutagenic and carcinogenic compound, is widely studied in the meat industry and lipid peroxidation research due to its implications for food quality and safety. Current methods for quantifying MDA in solid tissues are labor-intensive, requiring multiple instruments and approximately two hours to complete. This study presents an ultrafast kinetic fluorogenic method for quantifying MDA in ground beef, utilizing 2-thiobarbituric acid (TBA) as a fluorogenic probe. The total assay time is significantly shortened to 6 min from sample preparation to data acquisition. The assay’s robustness against matrix interference was validated using sample volume variation and standard addition calibration methods. Additionally, the effects of ambient exposure to air, washing, and cooking on MDA content in raw ground beef were quantified. While both ambient exposure to air and cooking increased MDA levels, washing raw ground beef and decanting cooked ground beef broth effectively reduced MDA levels in the ground beef. This simple and rapid assay can be adopted both in food research and industry. Moreover, insights from our study on the relationship between ground beef treatment and MDA concentration will help consumers make informed decisions about ground beef handling and consumption to lower their intake of MDA. Full article

Background: TNBC patients respond poorly to chemotherapy, leading to high mortality rates and a worsening prognosis. Here, we investigated the effect of M-I on TNBC tumor growth suppression and its potential mechanisms. Methods: Signaling pathways were analyzed to study the effect of M-I on TNBC cells (human MDA-MB-231 and mouse 4T1). We used orthotopic mouse models to examine the anti-tumor efficacy of M-I. Tumor volume and the status of tumor-associated macrophages (TAMs) were assessed by qRT-PCR or FACS analysis. Results: We found a significant dose- and time-dependent inhibition of TNBC cell proliferation following treatment with M-I. Cell cycle analysis revealed a shortened S phase in M-I-treated cells and downregulation of AURKA, PLK1, CDC25c, CDK1, and cyclinB1. Furthermore, M-I treatment reduced the expression of pSTAT3, cyclinD1, and c-Myc in TNBC cells. To evaluate the anti-tumor efficacy of M-I, we employed orthotopic TNBC mouse models and observed a significant reduction in tumor growth without measurable toxicity. Next, we analyzed RNA from control and M-I-treated tumors to further assess the status of TAMs and observed a significant decrease in M2-like macrophages in the M-I-treated group. Immortalized bone marrow-derived mouse macrophages (iMacs) exposed to conditioned media (CM) of TNBC cells with or without M-I treatment indicated that the M-I treated CM of TNBC cells significantly reduce the M2phenotype in iMacs. Mechanistically, we found that M-I specifically targets the IL-4/MAPK signaling axis to reduce immunosuppressive M2 macrophage polarization. Conclusions: Our study reveals a novel mechanism by which M-I inhibits TNBC cell proliferation by regulating intracellular signaling and altering TAMs in the tumor microenvironment and highlights its potential as a promising candidate for TNBC therapy. Full article

Background: This study investigates the potential mechanisms behind changes in cardiac structure and function in long COVID patients. Methods: This study involved 176 consecutive outpatients in follow-up care (average age 55.9 years; 58.5% male) who experienced symptoms for over 12 weeks (average 6.2 ± 2.7 months), following coronavirus infection (COVID-19). Results: The patients with long COVID and cardiovascular manifestations were significantly more hospitalized (88.5% vs. 75.9%) and had longer hospital stays. Significant echocardiography changes were observed in the left ventricular ejection fraction (LVEF) (59.6 ± 5.4% vs. 62.5 ± 3.8%); longitudinal strain (LS) in the sub-endocardium and intra-myocardium layers (−20.9 vs. −22.0% and −18.6 vs. −19.5%); circumferential strain (CS) in the sub-epicardium layers (−9.6 vs. −10.5%); and CS post-systolic shortening (CS PSS) (0.138 vs. 0.088 s). Additionally, pathological cardiac magnetic resonance (CMR) findings were seen in 58.2% of the group of patients with long COVID and cardiovascular manifestation; 43.3% exhibited positive late gadolinium enhancement (LGE), 21.0% had elevated native T1 mapping, and 22.4% had elevated native T2 mapping. Conclusions: Most patients with long COVID showed structural and functional changes in their cardiovascular systems, primarily caused by prolonged inflammation. Using multimodality imaging is important for uncovering the mechanisms to predict chronic myocarditis, early-stage heart failure, and pre-ischemic states, which can lead to serious complications. Recognizing the specific cardiovascular phenotypes associated with long COVID is essential in order to provide timely and appropriate treatment. Full article

A challenge in orthodontic treatment is the long time taken to move teeth, which extends the long treatment period. Accordingly, various treatment protocols and orthodontic materials have been developed to shorten the orthodontic treatment period. However, controlling biological reactions is considered necessary to further shorten this treatment period. Orthodontic force results in compression of the periodontal ligament in the direction of tooth movement, resulting in various reactions in the periodontal ligament that induce osteoclast development, alveolar bone absorption, and teeth movement. The aforementioned reactions include immune reactions. Cytokines are substances responsible for intercellular communication and are involved in various physiological actions, including immune and inflammatory reactions. They cause various cellular responses, including cell proliferation, differentiation, cell death, and functional expression. Various cytokines are involved in biological reactions during orthodontic tooth movement (OTM). It is important to understand the role of cytokines during OTM in order to elucidate their biological response. This review discusses the role of cytokines during OTM. Full article

Background/Objectives: Antibiotic resistance is a significant and escalating challenge that limits available therapeutic options. This issue is further exacerbated by the decreasing number of new antibiotics being developed. Our study aims to describe the epidemiology and pattern of antibiotic resistance in Gram-negative infections isolated from a cohort of hospitalized patients and to analyze the distribution of infections within the hospital setting. Methods: A retrospective study was conducted on all patients admitted to Vito Fazzi Hospital in Lecce, Italy, who required an infectious disease consultation due to the isolation of Gram-negative bacteria from 1 January 2018 to 31 December 2022. Results: During the study period, 402 isolates obtained from 382 patients (240 men and 142 women) with infections caused by Gram-negative bacteria were identified. Among these isolated, 226 exhibited multidrug resistance, defined as resistance to at least one antimicrobial agent from three or more different classes. In 2018, the percentage of multidrug-resistant isolates peaked at 87.6%, before decreasing to the lowest level (66.2%) in 2021. Overall, of the 402 isolates, 154 (38.3%) displayed resistance to carbapenems, while 73 (18.1%) were resistant to extended-spectrum beta-lactamases (ESBLs). Among the resistant microorganisms, Klebsiella pneumoniae showed the highest resistance to carbapenems, accounting for 85.2% of all resistant strains. Escherichia coli exhibited the greatest resistance to ESBLs, with a rate of 86.7%. Among carbapenem-resistant K. pneumoniae isolates, the following resistance rates were observed: KPC-1 at 98.2%, IMP-1 at 0.9%, VIM-1 at 0.9%, and NDM-1 at 0.9%. Conclusions: Patients with infections caused by multidrug-resistant bacteria have limited treatment options and are therefore at an increased risk of death, complications, and longer hospital stays. Rapid diagnostic techniques and antimicrobial stewardship programs—especially for ESBLs and carbapenemases—can significantly shorten the time needed to identify the infection and initiate appropriate antimicrobial therapy compared to traditional methods. Additionally, enhancing surveillance of antimicrobial resistance within populations is crucial to address this emerging public health challenge. Full article

This study presents a comprehensive treatment system for addressing leakage challenges in underground structure construction within complex karst terrains, demonstrated through the case of Baiyun Station in Guangzhou. Integrating advanced geological investigation, dynamic grouting techniques, and adaptive structural remediation strategies, this methodology effectively mitigates water inflow risks in structurally heterogeneous karst environments. Key innovations include the “one-trench two-drilling” exploration-grouting system for karst cave detection and filling, a multi-stage emergency water-gushing control protocol combining cofferdam sealing and dual-fluid grouting, and a zoned epoxy resin injection scheme for structural fissure remediation. Implementation at Baiyun Station achieved quantifiable outcomes: karst cave filling rates increased from 35.98% to 82.6%, foundation pit horizontal displacements reduced by 67–68%, and structural seepage repair rates reached 96.4%. The treatment system reduced construction costs by CNY 12 million and shortened schedules by 45 days through optimized pile formation efficiency (98% qualification rate) and minimized rework. While demonstrating superior performance in sealing > 0.2 mm fissures, limitations persist in addressing sub-micron fractures and ensuring long-term epoxy resin durability. This research establishes a replicable framework for underground engineering in karst regions, emphasizing real-time monitoring, multi-technology synergy, and environmental sustainability. Full article

With the development of the agro-processing industry, the efficient cryogenic treatment and resource utilization of porcine bile—a high-value byproduct—has received increasing attention. This study investigates the dynamic behaviour and freezing characteristics of porcine bile droplets upon impact on cold substrates under varying conditions of surface temperature (−10 °C to −20 °C) and impact velocity (0.18–0.59 m/s). The effects of droplet size, dimensionless numbers (Weber, Reynolds, Bond, Ohnesorge, and Prandtl), and thermal gradients were systematically analyzed. A thermoelectric cooling substrate combined with high-speed imaging was used to quantitatively characterize the spreading ratio, retraction ratio, and freezing time of droplets. The results show that the maximum spreading ratio increases with higher impact velocity but decreases with lower substrate temperature. Lower substrate temperatures significantly shorten the freezing time, with a maximum reduction of up to 45%, particularly for smaller droplets. Droplets with high Weber numbers (We > 3) form flattened ice layers with preserved retraction patterns, while those with low Weber numbers (We < 1) generate smooth, hemispherical ice caps. For the first time, the thermophysical properties of porcine bile were incorporated into the framework of droplet impact dynamics on cryogenic surfaces. The findings reveal multiscale freezing mechanisms of biological fluids at low temperatures and provide a theoretical basis for optimizing processes such as freeze-drying and cryogenic sterilization in agro-product processing. Full article

This article studies the effects of a laser remelting treatment on the microstructure and properties of Al-Cu-Mn alloy surfaces, as well as the effects of a heat treatment process on the microstructure and mechanical properties of the matrix zone and remelting zone. The results showed that the remelting zone structure was mainly composed of equiaxed dendrites and fine columnar dendrites. The α(Al) phase and θ(Al₂Cu) phase were greatly refined after laser remelting. The T(Al₁₂CuMn₂) phase was completely dissolved into the α(Al) matrix. The hardness of the remelting zone increased significantly with an increase in the height of the molten pool, and the strengthening mechanism was mainly fine grain strengthening and second phase strengthening. For identical aging treatments, the solution treatment at 530 °C for 4 h yielded the highest hardness. Relative to samples aged without prior solution treatment, hardness increased by 80% in the matrix zone and 59.1% in the remelting zone. When the solid solution process was the same, the time to reach peak hardness was shortened when the aging temperature increased, and the hardness of both the matrix zone and remelting zone reached its peak at 175 °C for 8 h of aging. After aging, the friction coefficient of the alloy decreased due to the increase in the strength of the alloy. Full article

Background: Delirium is a frequent yet pathophysiologically still poorly understood complication in the intensive care unit (ICU) and is associated with adverse outcomes for the patients. Currently, guidelines give several recommendations for treating delirium in the ICU, but to date no sufficient drug treatment exists. Dexmedetomidine, primarily used for anesthesia and sedation in ICUs has shown a preventive effect of delirium compared to other sedatives, such as propofol. We hypothesize that overnight administration of dexmedetomidine may prevent and/or shorten the duration of delirium in ICU patients. Methods: The Basel propofol dexmedetomidine (BaProDex) Study was a single-center, prospective, randomized controlled trial. We included adult ICU patients with hyperactive or mixed delirium. Patients with delirium prior to ICU admission, advanced heart block, uncontrolled hypotension, or status epilepticus were excluded. The participants were randomly assigned 1:1 to either receive dexmedetomidine (study group) or propofol (control group) as a continuous infusion overnight. The Intensive Care Delirium Screening Checklist (ICDSC) was applied at least three times per day. Delirium was defined as an ICDSC ≥ 4. The study drug was administered until the end of delirium or ICU discharge. The primary endpoint was the time to delirium episode end, which was analyzed using cumulative incidence curves and a cause specific Cox proportional hazards regression with death as a competing risk. Secondary endpoints included recurrence of delirium until 28 days after ICU discharge, death until day 28, severity of ICU delirium, number of ventilation days, ICU length of stay (LOS) in hours, hospital length of stay in days and survival after three and twelve months after ICU discharge. Due to insufficient recruitment the trial needed to be stopped prematurely. Results: In total, 38 patients were enrolled and randomized in the two groups. The median duration of delirium was shorter in the dexmedetomidine group as compared to the propofol group (ITT: 34 vs. 66 h; PP: 31 vs. 66 h), resulting in a hazard ratio of 1.92 (95% CI 0.89–4.15, p = 0.097) in the ITT and 2.95 (95% CI 1.27–6.86, p = 0.012) in the PP analysis. In the PP analysis, the 28-day mortality was lower in the dexmedetomidine group (1 vs. 5 deaths) and fewer patients needed ventilation (7 vs. 15 patients). Both ICU and hospital LOS were shorter in the dexmedetomidine group (ICU LOS: median 43 vs. 128 h; hospital LOS: median 12 vs. 22 days). Further, mortality up to three and twelve months was lower in the dexmedetomidine group compared to the propofol group (PP: 2 vs. 8 patients died within twelve months, 2 vs. 7 patients died within three months). The recurrence of delirium until 28 days after ICU discharge and severity of delirium were similar in both groups. Conclusions: Despite premature termination, BaProDex provides preliminary evidence for a reduction in the duration of delirium by nocturnal infusion of dexmedetomidine compared to propofol. Therefore, dexmedetomidine may be considered an option to treat hyperactive or mixed delirium in ICU patients. However, due to the small sample size, the study is rather of exploratory nature due to the premature termination, and we cannot rule out that the observed treatment effect is overly optimistic or by chance. Full article

Methicillin-resistant Staphylococcus aureus (MRSA) represents a major therapeutic challenge due to its multidrug-resistance and the associated clinical burden. Daptomycin (DAP), a cyclic lipopeptide antibiotic, has become a key agent for the treatment of severe MRSA infections owing to its rapid bactericidal activity and favourable safety profile. In this narrative review, we examine studies published between 2010 and April 2025. The data suggest that treatment with high-dose (8–10 mg kg⁻¹) DAP shortened the time to blood-culture sterilisation by a median of 2 days compared with standard-dose vancomycin without increasing toxicity when model-informed area-under-the-curve monitoring was employed. Particular attention is given to the synergistic effects of DAP combined with fosfomycin or β-lactams, especially ceftaroline and ceftobiprole, in overcoming persistent and refractory MRSA infections; this approach results in a reduction in microbiological failure relative to monotherapy. Resistance remains uncommon (<2% of isolates), but recurrent mutations in mprF, liaFSR, and walK underscore the need for proactive genomic surveillance. Despite promising preclinical and clinical evidence supporting combination strategies, further randomized controlled trials are necessary to establish their definitive role in clinical practice, as are head-to-head cost-effectiveness evaluations. DAP remains a critical option in the evolving landscape of MRSA management, provided its use is integrated with precision dosing, resistance surveillance, and antimicrobial-stewardship frameworks. Full article

Moisture–electricity generators (MEGs) hold great promise for green energy conversion. However, existing devices focus on the need for complex gradient distribution treatments and the improvement in output voltage, overlooking the important role of the graphene oxide (GO) oxidation degree and the response time and recovery time in practical application. In this work, we develop printed MEGs by synthesizing reduced graphene oxide/silver nanoparticle (rGO/Ag) composites and controlling the GO oxidation degree. The rGO/Ag layer serves as a functional component that enhances cycling stability and shortens the recovery time. Additionally, compared to conventional rigid-structure devices, these flexible MEGs can be produced by inkjet printing and drop-casting techniques. A 1 cm² MEG can generate a voltage of up to 60 mV within 2.4 s. Notably, higher output voltages can be easily achieved by connecting multiple MEG units in series, with 10 units producing 200 mV even under low relative humidity (RH). This work presents a low-cost, highly flexible, lightweight, and scalable power generator, paving the way for broader applications of GO and further advancement of MEG technology in wearable electronics, respiratory monitoring, and Internet of Things applications. Full article