Search Results (266)

Tagetes patula is a widely cultivated ornamental plant and a natural source of bioactive compounds. This study evaluated the effects of cultivation–substrate systems on growth, flowering, lutein and zeaxanthin accumulation, substrate microbiological properties, and pest and disease occurrence in three T. patula cultivars (‘Csemő’, ‘Robusta kénsárga’, and ‘Orion’) grown under two greenhouse (peat-based substrate and hydroponics) and three field conditions (peat-based and two peat-free substrates). Greenhouse hydroponics markedly enhanced vegetative growth, resulting in the highest plant height, stem diameter, and shoot biomass, whereas peat-based greenhouse substrates produced the lowest vegetative performance. Flowering responses were more moderate and largely cultivar-dependent: peat-based field conditions supported the highest inflorescence numbers, cv. ‘Orion’ produced the greatest inflorescence biomass, and cv. ‘Robuszta kénsárga’ showed the strongest flowering intensity in peat-based systems. Cultivar ‘Csemő’ consistently accumulated the highest lutein and zeaxanthin concentrations among cultivars. Substrate moisture and microbial activity differed substantially among systems, with peat-free substrates frequently exhibiting elevated enzymatic activity. No fungal diseases were detected; thrips occurred only in greenhouse systems, and spider mites were restricted to cv. ‘Orion’ under hydroponic conditions. Overall, hydroponic and peat-free systems enhanced vegetative growth and microbial activity, whereas flowering and carotenoid accumulation were primarily cultivar-specific, as further supported by correlation analysis and PCA. These findings demonstrate that sustainable peat alternatives and hydroponic systems can effectively support high-quality T. patula production and carotenoid yield. Full article

Against the backdrop of China’s “Dual Carbon” strategy (peak carbon emissions and carbon neutrality), timber forests serve the dual function of wood supply and carbon sink enhancement. In this study, we employed the Kuenm package in R to optimize Maximum Entropy model (MaxEnt) parameters. Based on the distribution data of six timber tree species in Sichuan Province and 43 environmental factors, we utilized the MaxEnt outputs and ArcGIS 10.8 software to map the geographic distribution of the suitable habitats for these species from the present day into the future (2061–2080) under different climate scenarios (SSP126 and SSP585). Furthermore, we analyzed the migration trend of their future distribution centers. The model optimization significantly improved both fit and predictive performance, with AUC values ranging from 0.8552 to 0.9637 and TSS values ranging from 0.6289 to 0.84, indicating high predictive capability and stability of the model. Analysis of environmental factors, including altitude, precipitation, and temperature, revealed that altitude plays a dominant role in species distribution. Future climate scenario simulations indicated that climate change will significantly alter the distribution of suitable habitats for these timber tree species. The suitable areas for some species contracted, with changes being particularly pronounced under the SSP585 scenario, in which the high-suitability area for Phoebe zhennan is projected to increase from 12,788 km² to 20,004 km², whereas the high-suitability area for Eucalyptus robusta is expected to contract from 8706 km² to 7715 km². The migration distances of suitable habitats for timber tree species in Sichuan range from 5 km to 101 km southwestward under different climate scenarios, and these shifts are statistically significant (p < 0.01), with shifts in elevation and precipitation patterns, reflecting species-specific responses to climate change. This study aims to predict future suitable habitats of timber tree species in Sichuan, providing scientific support for forestry planning, forest quality improvement, and climate risk mitigation. Full article

For 12 isolates of Ilyonectria mors-panacis and 4 isolates of Ilyonectria robusta, the number of genes in the secretome showed a negative correlation with growth rates in culture, especially for small secreted non-cysteine-rich and cysteine-rich proteins, and several proteases and lipases, while it was positively correlated with genes for six CAZyme classes/modules and other proteases and lipases. However, this significant correlation with growth rate was influenced by the I. robusta isolates mostly having faster growth rates than the I. mors-panacis isolates on PDA, indicating a species-level difference. The only significant relationship of gene number to virulence was a positive correlation with genes for secreted glycoside hydrolases in families 18 and 78, and this was related to differences between isolates, even if only I. mors-panacis isolates were examined, indicating a difference within species. Glycoside hydrolase family 18 includes chitinase-like proteins, endo-β-N-acetylglucosaminidases, lectins, and xylanase inhibitors, which could help suppress triggered immunity by the host and regulate fungal xylanase activity. Glycoside hydrolase family 78 contain α-L-rhamnosidases that can cleave flavonoid glycosides, saponins, and ginsenosides, which could degrade antimicrobial compounds produced as a host response during infection. These results indicate that the number of certain classes of secreted enzymes could be a factor in both growth rate in culture and virulence. Full article

This study evaluated the extraction efficiency of two Natural Deep Eutectic Solvents (NADESs), glycerol–urea (1:1) and citric acid–sorbitol (1:2), for recovering phenolic compounds from the different parts of the fruit (pulp, seed-containing pulp, seeds, and peel) of Opuntia robusta and Opuntia ficus-indica in comparison with 50% methanol. Phytochemical profiling was performed using ultra-high-performance liquid chromatography–high-resolution mass spectrometry, alongside antioxidant and enzyme inhibition assessments (acetylcholinesterase, butyrylcholinesterase, tyrosinase, α-glucosidase, and α-amylase). Glycerol–urea performed similarly to methanol in extracting phenolic compounds with notable antioxidant properties. Peel extracts contained the highest levels of bioactive compounds, particularly phenolic acids (525.49 in O. robusta and 362.96 µg/g_DW in O. ficus indica). Enzyme inhibition varied across species and fruit parts, with extracts from both species inhibiting all targeted enzymes. Notably, this study provides the first evidence of tyrosinase inhibitory activity in O. robusta, which exhibited the strongest inhibition. Overall, these results emphasize the potential of cactus fruit extracts, particularly from O. robusta, for valorization, and support the use of NADESs as a sustainable and medium for extracting antioxidant compounds. Furthermore, the potential of fruit peel as waste with nutraceutical applications was demonstrated. Full article

Accurate biomass and carbon estimation in tropical plantation forests requires species-specific allometric models. Silver Oak (Grevillea robusta A. Cunn. ex R. Br.), cultivar “AVAONE,” is widely planted in northeastern Thailand, yet locally calibrated equations remain limited. This study developed species- and site-specific allometric models using destructive sampling of eight trees (n = 8) aged 2–9 years from a single plantation in Pak Chong District, Nakhon Ratchasima Province, without independent validation. Each tree was separated into stem, branches, leaves, and roots to determine fresh and dry biomass, and carbon concentrations were measured using a LECO CHN628 analyzer in an ISO/IEC 17025-accredited laboratory. Aboveground biomass increased from 17.49 kg at age 2 to 860.42 kg at age 9, with the most rapid gains occurring between ages 6 and 9. Tree height stabilized at approximately 19–20 m after age 7, while diameter continued to increase. Stems accounted for the largest proportion of dry biomass, followed by branches and roots. Carbon concentrations ranged from 45.561% to 48.704%, close to the IPCC default value of 47%. Power-law models based on D²H showed clear relationships with biomass, with R² values ranging from 0.7365 to 0.9372 for individual components and 0.8409 for aboveground biomass. These locally derived equations provide preliminary, site-specific relationships for estimating biomass and carbon stocks in Silver Oak AVAONE plantations and offer a baseline for future studies with expanded sampling and independent validation. Full article

To promote a sustainable coffee industry, Davao Oriental has branded its coffee in association with Mt. Hamiguitan. This study profiles the production capacities and agricultural practices of smallholder coffee farmers along the Mt. Hamiguitan buffer zone, focusing on women’s roles and farmers’ climate change awareness and adaptation strategies. Fifty-seven purposively selected farmers (28 females, 29 males) were interviewed using a validated semi-structured questionnaire. Data were analyzed using thematic analysis and descriptive statistics. Most farmers (70%) were old (≥51) with over 40 years of experience (51%). Non-participation in farmers’ associations (73.7%) surpassed active membership (26.3%), with only 14% attending training. Most farmers (65%) intercropped 500–600 Robusta coffee trees/ha, with many farms established in the 1980s (36%). The farmers performed clearing (82%) and pruning (94%) of their coffee. Women played a vital role, especially during drying and harvesting. All respondents acknowledged climate change and employed practices such as intercropping, pruning, using ipil-ipil for shade, and utilizing nets, which, though originally aimed at improving crop resilience, can also serve as adaptations to mitigate the impacts of climate change. Recommendations for fostering sustainable practices while improving livelihoods include joining cooperatives, providing training for enhanced climate change adaptation strategies, and increasing women’s participation in coffee production. Full article

Zinc oxide nanoparticles (ZnO NPs) have garnered increasing attention in agriculture due to their potential to enhance plant growth and nutrient use. This research investigates the concentration-dependent effects of ZnO NPs on young crabapple (Malus robusta) plants, addressing gaps in understanding how different concentrations influence plant development. A hydroponic experiment was conducted, applying foliar treatments of 200 mg L⁻¹ ZnSO₄ (S200) and 200, 500, and 1000 mg L⁻¹ ZnO NPs (N200, N500, N100). The control group (CK) was treated with deionized water (dH₂O). Growth parameters, antioxidant enzyme activity, and nutrient contents were measured to evaluate the impact of ZnO NPs on plant development and nutrient uptake. The results showed that N200 enhanced growth, increasing plant height by 22.64%, total dry weight by 49.36%, and root length by 116.07%. In contrast, N500 and N1000 induced oxidative stress, elevating H₂O₂ and MDA by 32.02~54.43% and inhibiting growth. N200 also improved nutrient uptake, increasing K, Ca, Fe, and Zn uptake fluxes by 84.92%, 112.12%, 185.15%, and 149.92%, respectively, whereas N1000 suppressed overall nutrient uptake but increased root Ca accumulation by 64.59%. These findings suggest that ZnO NPs can enhance plant growth and nutrient utilization at low concentrations, with potential implications for agricultural practices involving nanoparticle (NP)-based fertilizers. Full article

Community-managed forests within agroforestry landscapes are vital for both carbon sequestration and agricultural sustainability. This study assesses the Hariyali Community Forest (HCF) in western Nepal, emphasizing its role in carbon storage within a Sal (Shorea robusta)-dominated lowland forest containing diverse native and medicinal species. Stratified field inventories combined with satellite-derived biomass and land-use/land-cover data were used to quantify carbon stocks and spatial trends. In 2022, the mean aboveground carbon density was 165 tC ha⁻¹, totaling approximately 101,640 tC (~373,017 tCO₂e), which closely matches satellite-based trends and indicates consistent carbon accumulation. Remote sensing from 2015–2022 showed a net tree cover gain of 427 ha compared to a 2000 baseline of 188 ha, evidencing effective community-led regeneration. The 615 ha Sal-dominated landscape also sustains agroforestry, small-scale horticulture, and subsistence crops, integrating livelihoods with conservation. Temporary carbon declines between 2020 and 2022, linked to localized harvesting and management shifts, highlight the need for stronger governance and local capacity. This study, among the first integrated carbon assessments in Nepal’s lowland Sal forests, demonstrates how community forestry advances REDD+ (Reducing Emissions from Deforestation and Forest Degradation, and the role of conservation, sustainable management of forests, and enhancement of forest carbon stocks in developing countries) objectives while enhancing rural resilience. Linking field inventories with satellite-derived biomass and land-cover data situates community forestry within regional environmental change and SDG (Sustainable Development Goals) targets (13, 15, and 1) through measurable ecosystem restoration and livelihood gains. Full article

Within the freshwater fish order Cypriniformes, loaches form a monophyletic lineage comprising nine families with more than 1400 species. Secondary sexual dimorphism is widespread among loach families, most notably in the form of enlarged pectoral fins and tubercles or other hard structures on the pectoral fin in adult males. To date, the family Botiidae, phylogenetically sister to all other loach families, was reported to lack such sexually dimorphic structures, leading to the hypothesis that the common ancestor of loaches did not exhibit sexual dimorphism. Here, we report the presence of sexual dimorphism in eight species of Botiidae: Leptobotia bellacauda, L. guilinensis, L. microphthalma, L. taeniops, L. tchangi, Parabotia fasciatus, Sinibotia pulchra, and S. robusta. In all species, adult males possess longer pectoral fins than females. Additionally, males of L. guilinensis and L. tchangi exhibit larger pelvic fins, while males of L. microphthalma have larger anal fins. In L. bellacauda, L. microphthalma, and L. tchangi, portions of the dorsal surface of the pectoral fin bear rows of tubercles. The three genera displaying sexual dimorphism belong to two different subfamilies, demonstrating that sexual dimorphism is widespread across Botiidae and not restricted to a single genus or subfamily. Our results show that sexual dimorphism is present in the most basal family of loaches, suggesting that it represents a synapomorphy of loach fishes. Full article

Grafting is a strategy to mitigate biotic and abiotic stresses in coffee systems. However, initial compatibility indicators between Coffea arabica scions and C. canephora rootstocks under controlled conditions remain insufficiently documented. We evaluated the physiological and morphological compatibility of four C. arabica cultivars (Bourbon, Geisha, Catuai, and Villa Sarchí) grafted onto C. canephora (Robusta) rootstock in a tropical highland nursery in the Peruvian Amazon. Seven physiological and six morphological variables were measured. Statistical analyses included one-way ANOVA and Kruskal–Wallis tests with Bonferroni post hoc comparisons. Two physiological parameters were significantly higher in Villa Sarchí grafts than in Robusta: PSII quantum yield (+0.044 units; p < 0.05) and electron transport rate (+14.702 µmol e⁻ m⁻² s⁻¹; p < 0.05); by contrast, net photosynthesis, stomatal conductance, intercellular CO₂ concentration, and transpiration did not differ, and maximum PSII efficiency was similar among treatments (p = 0.509). Conversely, no morphological trait showed significant differences, and graft-take was high across all combinations. The results support the use of Coffea robusta as a rootstock for these four cultivated varieties, thereby offering the possibility of improving their resilience in tropical highland regions. Full article

The aim of this study was to compare, under standardized conditions, the content and molecular properties of carbohydrates occurring in extracts from roasted coffee beans and in coffee substitutes made from roasted chicory root, barley, wheat, spelt, and rye. The study revealed an over 8% higher carbohydrate content, primarily polysaccharides of a molar mass greater than 1800 g/mol, in instant Arabica coffee extract compared to Robusta coffee. Significant differences were also demonstrated in the carbohydrate composition of Arabica and Robusta coffee extracts, as well as coffees obtained using laboratory and industrial methods. Coffee substitutes generally contained more polysaccharides and two to five times more oligosaccharides of a molar mass ranging from 400 to 1800 g/mol, and consequently, total carbohydrates, compared to coffee extracts. The high oligosaccharide contents (11–25%) of very diverse monosaccharide composition found in instant coffee substitutes indicate the potential prebiotic effects of these products. The highest melanoidin content among instant coffee extracts and coffee substitutes was found in a coffee substitute made from roasted chicory. Furthermore, extract from Arabica coffee contained higher amounts of melanoidins than Robusta coffee. Full article

The chemical profile of coffee depends on numerous factors, the complexity of which makes it difficult to clearly assess their influence. The aim of this study was to comprehensively evaluate the impact of selected coffee brewing methods (Espresso, Simple Infusion, French Press, V60), taking into account the coffee species (Arabica, Robusta, Blends), the degree of roasting (light, medium, dark) and the geographical origin (single-origin and multi-origin) on the chemical composition of the brew. Eighteen different types of coffee, which differ in the aforementioned characteristics, were analyzed. The caffeine content (using high-performance liquid chromatography), the total phenolic content (TPC; using a spectrophotometric method), and selected minerals (calcium, iron, potassium, magnesium, sodium, phosphorus, zinc; using Inductively Coupled Plasma–Optical Emission Spectrometry) were analyzed. The analysis showed that both the brewing method and the species had a significant influence on the chemical profile of the resulting brews, while the degree of roasting and the origin showed no significant influence. The Espresso method showed the highest caffeine, TPC, potassium, magnesium, and phosphorus content, the V60 method—calcium, iron, and sodium, and the French Press and Simple Infusion methods showed intermediate values. Robusta coffee contained more caffeine and TPC, Arabica contained more magnesium, and Blend showed medium values for both species. The results obtained may have practical implications for both consumers and the coffee industry, supporting informed decision-making and the refinement of brewing methods. Full article

This study investigates the solubility correlation of oil extracted from Sabah green Robusta coffee (Coffea canephora) beans through supercritical carbon dioxide (SC-CO₂) extraction. Sabah, recognized as the largest coffee-producing region in Malaysia, serves as a significant source of Robusta beans for this research. The solubility of coffee bean oil was evaluated under varying pressures (10–30 MPa) and temperatures (40–80 °C). The maximum solubility, 2.681 mg/g CO₂, was recorded at 30 MPa and 40 °C, whereas the lowest solubility, approximately 0.440 mg/g CO₂, occurred at 20 MPa and 80 °C. A clear inverse relationship between solubility and temperature was observed, with solubility decreasing as temperature increased to 80 °C. Conversely, elevated pressure, particularly at 30 MPa, enhanced solubility due to the increased density and solvent power of SC-CO₂. Experimental data exhibited strong agreement with Chrastil’s equation, yielding a relatively low percentage error of 3.37%, compared with 14.57% for the del Valle-Aguilera model. These findings demonstrate the reliability of Chrastil’s model in predicting the solubility of Sabah green coffee bean oil in SC-CO₂. Overall, the research highlights the potential of SC-CO₂ extraction as a sustainable, solvent-free approach for obtaining high-quality coffee oil extracts, with promising applications in the food industry and possible extension to the recovery of other bioactive compounds in food processing. Full article

Coffee silverskin (CS), the principal solid by-product from coffee roasting, is a promising raw material for sustainable food applications aligned with circular economy principles. Due to its high flammability at roasting temperatures, effective management of CS is not only an environmental but also a safety concern in coffee processing facilities. To the best of our knowledge, this is the first study evaluating the chemical composition, bioactivity, safety, and environmental impact of torrefacto (CT) and natural (CN) coffee silverskin. CT (from Arabica–Robusta blends subjected to sugar-glazing) and CN (from 100% Arabica) were characterized in terms of composition and function. Oven-dried CT showed higher levels of caffeine (13.2 ± 0.6 mg/g vs. 8.7 ± 0.7 mg/g for CN), chlorogenic acid (1.34 ± 0.08 mg/g vs. 0.92 ± 0.06 mg/g), protein (18.1 ± 0.2% vs. 16.7 ± 0.2%), and melanoidins (14.9 ± 0.3 mg/g vs. 9.6 ± 0.2 mg/g), but CN yielded more total phenolics (13.8 ± 0.6 mg GAE/g). Both types exhibited strong antioxidant capacity (ABTS: 48.9–59.2 µmol TE/g), and all oven-dried samples met food safety criteria (microbial loads below 10² CFU/g, moisture 7.9%). Oven drying was identified as the most industrially viable, ensuring preservation of bioactives and resulting in a 19% lower greenhouse gas emissions impact compared to freeze-drying. Sun drying was less reliable microbiologically. The valorization of oven-dried CT as a clean-label, antioxidant-rich colorant offers clear potential for food reformulation and waste reduction. Renewable energy use during drying is recommended to further enhance sustainability. This study provides scientific evidence to support the safe use of coffee silverskin as a novel food, contributing to regulatory assessment and sustainable food innovation aligned with SDGs 9, 12, and 13. Full article

The nutritional, mineral, and bioactive profiles of four Opuntia fruit varieties—Opuntia robusta red variety (OR-RV) and three Opuntia ficus-indica varieties (red, yellow, and green: OFI-RV, OFI-YV, and OFI-GV, respectively)—were characterized to assess their compositional diversity and potential discriminant markers. Standard analytical procedures were applied to determine proximate composition, individual sugars, fibre content, mineral concentration, and bioactive compounds, followed by antioxidant activity assays. Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were used to explore multivariate patterns and identify variables with the greatest discriminatory power. Results revealed significant inter-varietal differences across all measured parameters (p < 0.05). OR-RV displayed the highest non-fibre carbohydrate, protein, copper, and ascorbic acid contents, as well as superior antioxidant activity. OFI-GV stood out for its high soluble and insoluble fibre, magnesium, and strontium levels, while OFI-YV was characterized by elevated sodium and calcium, and OFI-RV by increased protein and glucose contents. LDA identified ascorbic acid, protein, and five mineral elements (Sr, Zn, Cu, Mn, B) as key discriminant variables, achieving 100% classification accuracy. These findings highlight compositional diversity among Opuntia varieties and support their differentiated use in food and health applications. Full article