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32 pages, 1435 KiB  
Review
Smart Safety Helmets with Integrated Vision Systems for Industrial Infrastructure Inspection: A Comprehensive Review of VSLAM-Enabled Technologies
by Emmanuel A. Merchán-Cruz, Samuel Moveh, Oleksandr Pasha, Reinis Tocelovskis, Alexander Grakovski, Alexander Krainyukov, Nikita Ostrovenecs, Ivans Gercevs and Vladimirs Petrovs
Sensors 2025, 25(15), 4834; https://doi.org/10.3390/s25154834 - 6 Aug 2025
Abstract
Smart safety helmets equipped with vision systems are emerging as powerful tools for industrial infrastructure inspection. This paper presents a comprehensive state-of-the-art review of such VSLAM-enabled (Visual Simultaneous Localization and Mapping) helmets. We surveyed the evolution from basic helmet cameras to intelligent, sensor-fused [...] Read more.
Smart safety helmets equipped with vision systems are emerging as powerful tools for industrial infrastructure inspection. This paper presents a comprehensive state-of-the-art review of such VSLAM-enabled (Visual Simultaneous Localization and Mapping) helmets. We surveyed the evolution from basic helmet cameras to intelligent, sensor-fused inspection platforms, highlighting how modern helmets leverage real-time visual SLAM algorithms to map environments and assist inspectors. A systematic literature search was conducted targeting high-impact journals, patents, and industry reports. We classify helmet-integrated camera systems into monocular, stereo, and omnidirectional types and compare their capabilities for infrastructure inspection. We examine core VSLAM algorithms (feature-based, direct, hybrid, and deep-learning-enhanced) and discuss their adaptation to wearable platforms. Multi-sensor fusion approaches integrating inertial, LiDAR, and GNSS data are reviewed, along with edge/cloud processing architectures enabling real-time performance. This paper compiles numerous industrial use cases, from bridges and tunnels to plants and power facilities, demonstrating significant improvements in inspection efficiency, data quality, and worker safety. Key challenges are analyzed, including technical hurdles (battery life, processing limits, and harsh environments), human factors (ergonomics, training, and cognitive load), and regulatory issues (safety certification and data privacy). We also identify emerging trends, such as semantic SLAM, AI-driven defect recognition, hardware miniaturization, and collaborative multi-helmet systems. This review finds that VSLAM-equipped smart helmets offer a transformative approach to infrastructure inspection, enabling real-time mapping, augmented awareness, and safer workflows. We conclude by highlighting current research gaps, notably in standardizing systems and integrating with asset management, and provide recommendations for industry adoption and future research directions. Full article
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23 pages, 3036 KiB  
Article
Research on the Synergistic Mechanism Design of Electricity-CET-TGC Markets and Transaction Strategies for Multiple Entities
by Zhenjiang Shi, Mengmeng Zhang, Lei An, Yan Lu, Daoshun Zha, Lili Liu and Tiantian Feng
Sustainability 2025, 17(15), 7130; https://doi.org/10.3390/su17157130 - 6 Aug 2025
Abstract
In the context of the global response to climate change and the active promotion of energy transformation, a number of low-carbon policies coupled with the development of synergies to help power system transformation is an important initiative. However, the insufficient articulation of the [...] Read more.
In the context of the global response to climate change and the active promotion of energy transformation, a number of low-carbon policies coupled with the development of synergies to help power system transformation is an important initiative. However, the insufficient articulation of the green power market, tradable green certificate (TGC) market, and carbon emission trading (CET) mechanism, and the ambiguous policy boundaries affect the trading decisions made by its market participants. Therefore, this paper systematically analyses the composition of the main players in the electricity-CET-TGC markets and their relationship with each other, and designs the synergistic mechanism of the electricity-CET-TGC markets, based on which, it constructs the optimal profit model of the thermal power plant operators, renewable energy manufacturers, power grid enterprises, power users and load aggregators under the electricity-CET-TGC markets synergy, and analyses the behavioural decision-making of the main players in the electricity-CET-TGC markets as well as the electric power system to optimise the trading strategy of each player. The results of the study show that: (1) The synergistic mechanism of electricity-CET-TGC markets can increase the proportion of green power grid-connected in the new type of power system. (2) In the selection of different environmental rights and benefits products, the direct participation of green power in the market-oriented trading is the main way, followed by applying for conversion of green power into China certified emission reduction (CCER). (3) The development of independent energy storage technology can produce greater economic and environmental benefits. This study provides policy support to promote the synergistic development of the electricity-CET-TGC markets and assist the low-carbon transformation of the power industry. Full article
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21 pages, 365 KiB  
Article
The Effect of Data Leakage and Feature Selection on Machine Learning Performance for Early Parkinson’s Disease Detection
by Jonathan Starcke, James Spadafora, Jonathan Spadafora, Phillip Spadafora and Milan Toma
Bioengineering 2025, 12(8), 845; https://doi.org/10.3390/bioengineering12080845 (registering DOI) - 6 Aug 2025
Abstract
If we do not urgently educate current and future medical professionals to critically evaluate and distinguish credible AI-assisted diagnostic tools from those whose performance is artificially inflated by data leakage or improper validation, we risk undermining clinician trust in all AI diagnostics and [...] Read more.
If we do not urgently educate current and future medical professionals to critically evaluate and distinguish credible AI-assisted diagnostic tools from those whose performance is artificially inflated by data leakage or improper validation, we risk undermining clinician trust in all AI diagnostics and jeopardizing future advances in patient care. For instance, machine learning models have shown high accuracy in diagnosing Parkinson’s Disease when trained on clinical features that are themselves diagnostic, such as tremor and rigidity. This study systematically investigates the impact of data leakage and feature selection on the true clinical utility of machine learning models for early Parkinson’s Disease detection. We constructed two experimental pipelines: one excluding all overt motor symptoms to simulate a subclinical scenario and a control including these features. Nine machine learning algorithms were evaluated using a robust three-way data split and comprehensive metric analysis. Results reveal that, without overt features, all models exhibited superficially acceptable F1 scores but failed catastrophically in specificity, misclassifying most healthy controls as Parkinson’s Disease. The inclusion of overt features dramatically improved performance, confirming that high accuracy was due to data leakage rather than genuine predictive power. These findings underscore the necessity of rigorous experimental design, transparent reporting, and critical evaluation of machine learning models in clinically realistic settings. Our work highlights the risks of overestimating model utility due to data leakage and provides guidance for developing robust, clinically meaningful machine learning tools for early disease detection. Full article
(This article belongs to the Special Issue Mathematical Models for Medical Diagnosis and Testing)
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18 pages, 7432 KiB  
Article
Design and Optimization of a Pneumatic Microvalve with Symmetric Magnetic Yoke and Permanent Magnet Assistance
by Zeqin Peng, Zongbo Zheng, Shaochen Yang, Xiaotao Zhao, Xingxiao Yu and Dong Han
Actuators 2025, 14(8), 388; https://doi.org/10.3390/act14080388 - 4 Aug 2025
Viewed by 143
Abstract
Electromagnetic pneumatic microvalves, widely used in knitting machines, typically operate based on a spring-return mechanism. When the coil is energized, the electromagnetic force overcomes the spring force to attract the armature, opening the valve. Upon de-energization, the armature returns to its original position [...] Read more.
Electromagnetic pneumatic microvalves, widely used in knitting machines, typically operate based on a spring-return mechanism. When the coil is energized, the electromagnetic force overcomes the spring force to attract the armature, opening the valve. Upon de-energization, the armature returns to its original position under the restoring force of the spring, closing the valve. However, most existing electromagnetic microvalves adopt a radially asymmetric magnetic yoke design, which generates additional radial forces during operation, leading to armature misalignment or even sticking. Additionally, the inductance effect of the coil causes a significant delay in the armature release response, making it difficult to meet the knitting machine’s requirements for rapid response and high reliability. To address these issues, this paper proposes an improved electromagnetic microvalve design. First, the magnetic yoke structure is modified to be radially symmetric, eliminating unnecessary radial forces and preventing armature sticking during operation. Second, a permanent magnet assist mechanism is introduced at the armature release end to enhance release speed and reduce delays caused by the inductance effect. The effectiveness of the proposed design is validated through electromagnetic numerical simulations, and a multi-objective genetic algorithm is further employed to optimize the geometric dimensions of the electromagnet. The optimization results indicate that, while maintaining the fundamental power supply principle of conventional designs, the new microvalve structure achieves a pull-in time comparable to traditional designs during engagement but significantly reduces the release response time by approximately 80.2%, effectively preventing armature sticking due to radial forces. The findings of this study provide a feasible and efficient technical solution for the design of electromagnetic microvalves in textile machinery applications. Full article
(This article belongs to the Section Miniaturized and Micro Actuators)
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20 pages, 12851 KiB  
Article
Evaluation of a Vision-Guided Shared-Control Robotic Arm System with Power Wheelchair Users
by Breelyn Kane Styler, Wei Deng, Cheng-Shiu Chung and Dan Ding
Sensors 2025, 25(15), 4768; https://doi.org/10.3390/s25154768 - 2 Aug 2025
Viewed by 226
Abstract
Wheelchair-mounted assistive robotic manipulators can provide reach and grasp functions for power wheelchair users. This in-lab study evaluated a vision-guided shared control (VGS) system with twelve users completing two multi-step kitchen tasks: a drinking task and a popcorn making task. Using a mixed [...] Read more.
Wheelchair-mounted assistive robotic manipulators can provide reach and grasp functions for power wheelchair users. This in-lab study evaluated a vision-guided shared control (VGS) system with twelve users completing two multi-step kitchen tasks: a drinking task and a popcorn making task. Using a mixed methods approach participants compared VGS and manual joystick control, providing performance metrics, qualitative insights, and lessons learned. Data collection included demographic questionnaires, the System Usability Scale (SUS), NASA Task Load Index (NASA-TLX), and exit interviews. No significant SUS differences were found between control modes, but NASA-TLX scores revealed VGS control significantly reduced workload during the drinking task and the popcorn task. VGS control reduced operation time and improved task success but was not universally preferred. Six participants preferred VGS, five preferred manual, and one had no preference. In addition, participants expressed interest in robotic arms for daily tasks and described two main operation challenges: distinguishing wrist orientation from rotation modes and managing depth perception. They also shared perspectives on how a personal robotic arm could complement caregiver support in their home. Full article
(This article belongs to the Special Issue Intelligent Sensors and Robots for Ambient Assisted Living)
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25 pages, 2515 KiB  
Article
Solar Agro Savior: Smart Agricultural Monitoring Using Drones and Deep Learning Techniques
by Manu Mundappat Ramachandran, Bisni Fahad Mon, Mohammad Hayajneh, Najah Abu Ali and Elarbi Badidi
Agriculture 2025, 15(15), 1656; https://doi.org/10.3390/agriculture15151656 - 1 Aug 2025
Viewed by 295
Abstract
The Solar Agro Savior (SAS) is an innovative solution that is assisted by drones for the sustainable utilization of water and plant disease observation in the agriculture sector. This system integrates an alerting mechanism for humidity, moisture, and temperature variations, which affect the [...] Read more.
The Solar Agro Savior (SAS) is an innovative solution that is assisted by drones for the sustainable utilization of water and plant disease observation in the agriculture sector. This system integrates an alerting mechanism for humidity, moisture, and temperature variations, which affect the plants’ health and optimization in water utilization, which enhances plant yield productivity. A significant feature of the system is the efficient monitoring system in a larger region through drones’ high-resolution cameras, which enables real-time, efficient response and alerting for environmental fluctuations to the authorities. The machine learning algorithm, particularly recurrent neural networks, which is a pioneer with agriculture and pest control, is incorporated for intelligent monitoring systems. The proposed system incorporates a specialized form of a recurrent neural network, Long Short-Term Memory (LSTM), which effectively addresses the vanishing gradient problem. It also utilizes an attention-based mechanism that enables the model to assign meaningful weights to the most important parts of the data sequence. This algorithm not only enhances water utilization efficiency but also boosts plant yield and strengthens pest control mechanisms. This system also provides sustainability through the re-utilization of water and the elimination of electric energy through solar panel systems for powering the inbuilt irrigation system. A comparative analysis of variant algorithms in the agriculture sector with a machine learning approach was also illustrated, and the proposed system yielded 99% yield accuracy, a 97.8% precision value, 98.4% recall, and a 98.4% F1 score value. By encompassing solar irrigation and artificial intelligence-driven analysis, the proposed algorithm, Solar Argo Savior, established a sustainable framework in the latest agricultural sectors and promoted sustainability to protect our environment and community. Full article
(This article belongs to the Section Agricultural Technology)
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40 pages, 2173 KiB  
Review
Bridging Genes and Sensory Characteristics in Legumes: Multi-Omics for Sensory Trait Improvement
by Niharika Sharma, Soumi Paul Mukhopadhyay, Dhanyakumar Onkarappa, Kalenahalli Yogendra and Vishal Ratanpaul
Agronomy 2025, 15(8), 1849; https://doi.org/10.3390/agronomy15081849 - 31 Jul 2025
Viewed by 717
Abstract
Legumes are vital sources of protein, dietary fibre and nutrients, making them crucial for global food security and sustainable agriculture. However, their widespread acceptance and consumption are often limited by undesirable sensory characteristics, such as “a beany flavour”, bitterness or variable textures. Addressing [...] Read more.
Legumes are vital sources of protein, dietary fibre and nutrients, making them crucial for global food security and sustainable agriculture. However, their widespread acceptance and consumption are often limited by undesirable sensory characteristics, such as “a beany flavour”, bitterness or variable textures. Addressing these challenges requires a comprehensive understanding of the complex molecular mechanisms governing appearance, aroma, taste, flavour, texture and palatability in legumes, aiming to enhance their sensory appeal. This review highlights the transformative power of multi-omics approaches in dissecting these intricate biological pathways and facilitating the targeted enhancement of legume sensory qualities. By integrating data from genomics, transcriptomics, proteomics and metabolomics, the genetic and biochemical networks that directly dictate sensory perception can be comprehensively unveiled. The insights gained from these integrated multi-omics studies are proving instrumental in developing strategies for sensory enhancement. They enable the identification of key biomarkers for desirable traits, facilitating more efficient marker-assisted selection (MAS) and genomic selection (GS) in breeding programs. Furthermore, a molecular understanding of sensory pathways opens avenues for precise gene editing (e.g., using CRISPR-Cas9) to modify specific genes, reduce off-flavour compounds or optimise texture. Beyond genetic improvements, multi-omics data also inform the optimisation of post-harvest handling and processing methods (e.g., germination and fermentation) to enhance desirable sensory profiles and mitigate undesirable ones. This holistic approach, spanning from the genetic blueprint to the final sensory experience, will accelerate the development of new legume cultivars and products with enhanced palatability, thereby fostering increased consumption and ultimately contributing to healthier diets and more resilient food systems worldwide. Full article
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13 pages, 3360 KiB  
Review
Technological Advances in Pre-Operative Planning
by Mikolaj R. Kowal, Mohammed Ibrahim, André L. Mihaljević, Philipp Kron and Peter Lodge
J. Clin. Med. 2025, 14(15), 5385; https://doi.org/10.3390/jcm14155385 - 30 Jul 2025
Viewed by 275
Abstract
Surgery remains a healthcare intervention with significant risks for patients. Novel technologies can now enhance the peri-operative workflow, with artificial intelligence (AI) and extended reality (XR) to assist with pre-operative planning. This review focuses on innovation in AI, XR and imaging for hepato-biliary [...] Read more.
Surgery remains a healthcare intervention with significant risks for patients. Novel technologies can now enhance the peri-operative workflow, with artificial intelligence (AI) and extended reality (XR) to assist with pre-operative planning. This review focuses on innovation in AI, XR and imaging for hepato-biliary surgery planning. The clinical challenges in hepato-biliary surgery arise from heterogeneity of clinical presentations, the need for multiple imaging modalities and highly variable local anatomy. AI-based models have been developed for risk prediction and multi-disciplinary tumor (MDT) board meetings. The future could involve an on-demand and highly accurate AI-powered decision tool for hepato-biliary surgery, assisting the surgeon to make the most informed decision on the treatment plan, conferring the best possible outcome for individual patients. Advances in AI can also be used to automate image interpretation and 3D modelling, enabling fast and accurate 3D reconstructions of patient anatomy. Surgical navigation systems utilizing XR are already in development, showing an early signal towards improved patient outcomes when used for hepato-biliary surgery. Live visualization of hepato-biliary anatomy in the operating theatre is likely to improve operative safety and performance. The technological advances in AI and XR provide new applications in pre-operative planning with potential for patient benefit. Their use in surgical simulation could accelerate learning curves for surgeons in training. Future research must focus on standardization of AI and XR study reporting, robust databases that are ethically and data protection-compliant, and development of inter-disciplinary tools for various healthcare applications and systems. Full article
(This article belongs to the Special Issue Surgical Precision: The Impact of AI and Robotics in General Surgery)
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19 pages, 3297 KiB  
Article
Secrecy Rate Maximization via Joint Robust Beamforming and Trajectory Optimization for Mobile User in ISAC-UAV System
by Lvxin Xu, Zhi Zhang and Liuguo Yin
Drones 2025, 9(8), 536; https://doi.org/10.3390/drones9080536 - 30 Jul 2025
Viewed by 160
Abstract
Unmanned aerial vehicles (UAVs) have emerged as a promising platform for integrated sensing and communication (ISAC) due to their mobility and deployment flexibility. By adaptively adjusting their flight trajectories, UAVs can maintain favorable line-of-sight (LoS) communication links and sensing angles, thus enhancing overall [...] Read more.
Unmanned aerial vehicles (UAVs) have emerged as a promising platform for integrated sensing and communication (ISAC) due to their mobility and deployment flexibility. By adaptively adjusting their flight trajectories, UAVs can maintain favorable line-of-sight (LoS) communication links and sensing angles, thus enhancing overall system performance in dynamic and complex environments. However, ensuring physical layer security (PLS) in such UAV-assisted ISAC systems remains a significant challenge, particularly in the presence of mobile users and potential eavesdroppers. This manuscript proposes a joint optimization framework that simultaneously designs robust transmit beamforming and UAV trajectories to secure downlink communication for multiple ground users. At each time slot, the UAV predicts user positions and maximizes the secrecy sum-rate, subject to constraints on total transmit power, multi-target sensing quality, and UAV mobility. To tackle this non-convex problem, we develop an efficient optimization algorithm based on successive convex approximation (SCA) and constrained optimization by linear approximations (COBYLA). Numerical simulations validate that the proposed framework effectively enhances the secrecy performance while maintaining high-quality sensing, achieving near-optimal performance under realistic system constraints. Full article
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18 pages, 1332 KiB  
Article
Optimization of Anthocyanin Extraction from Purple Sweet Potato Peel (Ipomea batata) Using Sonotrode Ultrasound-Assisted Extraction
by Raquel Lucas-González, Mirian Pateiro, Rubén Domínguez-Valencia, Celia Carrillo and José M. Lorenzo
Foods 2025, 14(15), 2686; https://doi.org/10.3390/foods14152686 - 30 Jul 2025
Viewed by 275
Abstract
Sweet potato is a valuable root due to its nutritional benefits, health-promoting properties, and technological applications. The peel, often discarded during food processing, can be employed in the food industry, supporting a circular economy. Purple sweet potato peel (PSPP) is rich in anthocyanins, [...] Read more.
Sweet potato is a valuable root due to its nutritional benefits, health-promoting properties, and technological applications. The peel, often discarded during food processing, can be employed in the food industry, supporting a circular economy. Purple sweet potato peel (PSPP) is rich in anthocyanins, which can be used as natural colourants and antioxidants. Optimising their extraction can enhance yield and reduce costs. The current work aimed to optimize anthocyanin and antioxidant recovery from PSPP using a Box-Behnken design and sonotrode ultrasound-assisted extraction (sonotrode-UAE). Three independent variables were analysed: extraction time (2–6 min), ethanol concentration (35–85%), and liquid-to-solid ratio (10–30 mL/g). The dependent variables included total monomeric anthocyanin content (TMAC), individual anthocyanins, and antioxidant activity. TMAC in 15 extracts ranged from 0.16 to 2.66 mg/g PSPP. Peonidin-3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside was the predominant anthocyanin. Among four antioxidant assays, Ferric-reducing antioxidant power (FRAP) showed the highest value. Ethanol concentration significantly influenced anthocyanin and antioxidant recovery (p < 0.05). The model demonstrated adequacy based on the coefficient of determination and variation. Optimal extraction conditions were 6 min with 60% ethanol at a 30 mL/g ratio. Predicted values were validated experimentally (coefficient of variation <10%). In conclusion, PSPP is a promising matrix for obtaining anthocyanin-rich extracts with antioxidant activity, offering potential applications in the food industry. Full article
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21 pages, 4865 KiB  
Article
Impact of Laser Power and Scanning Speed on Single-Walled Support Structures in Powder Bed Fusion of AISI 316L
by Dan Alexander Gallego, Henrique Rodrigues Oliveira, Tiago Cunha, Jeferson Trevizan Pacheco, Oksana Kovalenko and Neri Volpato
J. Manuf. Mater. Process. 2025, 9(8), 254; https://doi.org/10.3390/jmmp9080254 - 30 Jul 2025
Viewed by 275
Abstract
Laser beam powder bed fusion of metals (PBF-LB/M, or simply L-PBF) has emerged as one of the most competitive additive manufacturing technologies for producing complex metallic components with high precision, design freedom, and minimal material waste. Among the various categories of additive manufacturing [...] Read more.
Laser beam powder bed fusion of metals (PBF-LB/M, or simply L-PBF) has emerged as one of the most competitive additive manufacturing technologies for producing complex metallic components with high precision, design freedom, and minimal material waste. Among the various categories of additive manufacturing processes, L-PBF stands out, paving the way for the execution of part designs with geometries previously considered unfeasible. Despite offering several advantages, parts with overhang features require the use of support structures to provide dimensional stability of the part. Support structures achieve this by resisting residual stresses generated during processing and assisting heat dissipation. Although the scientific community acknowledges the role of support structures in the success of L-PBF manufacturing, they have remained relatively underexplored in the literature. In this context, the present work investigated the impact of laser power and scanning speed on the dimensioning, integrity and tensile strength of single-walled block type support structures manufactured in AISI 316L stainless steel. The method proposed in this work is divided in two stages: processing parameter exploration, and mechanical characterization. The results indicated that support structures become more robust and resistant as laser power increases, and the opposite effect is observed with an increment in scanning speed. In addition, defects were detected at the interfaces between the bulk and support regions, which were crucial for the failure of the tensile test specimens. For a layer thickness corresponding to 0.060 mm, it was verified that the combination of laser power and scanning speed of 150 W and 500 mm/s resulted in the highest tensile resistance while respecting the dimensional deviation requirement. Full article
(This article belongs to the Special Issue Recent Advances in Optimization of Additive Manufacturing Processes)
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21 pages, 1657 KiB  
Article
Heterogeneous-IRS-Assisted Millimeter-Wave Systems: Element Position and Phase Shift Optimization
by Weibiao Zhao, Qiucen Wu, Hao Wei, Dongliang Su and Yu Zhu
Sensors 2025, 25(15), 4688; https://doi.org/10.3390/s25154688 - 29 Jul 2025
Viewed by 239
Abstract
Intelligent reflecting surfaces (IRSs) have attracted extensive attention in the design of future communication networks. However, their large number of reflecting elements still results in non-negligible power consumption and hardware costs. To address this issue, we previously proposed a green heterogeneous IRS (HE-IRS) [...] Read more.
Intelligent reflecting surfaces (IRSs) have attracted extensive attention in the design of future communication networks. However, their large number of reflecting elements still results in non-negligible power consumption and hardware costs. To address this issue, we previously proposed a green heterogeneous IRS (HE-IRS) consisting of both dynamically tunable elements (DTEs) and statically tunable elements (STEs). Compared to conventional IRSs with only DTEs, the unique DTE–STE integrated structure introduces new challenges in optimizing the positions and phase shifts of the two types of elements. In this paper, we investigate the element position and phase shift optimization problems in HE-IRS-assisted millimeter-wave systems. We first propose a particle swarm optimization algorithm to determine the specific positions of the DTEs and STEs. Then, by decomposing the phase shift optimization of the two types of elements into two subproblems, we utilize the manifold optimization method to optimize the phase shifts of the STEs, followed by deriving a closed-form solution for those of the DTEs. Furthermore, we propose a low-complexity phase shift optimization algorithm for both DTEs and STEs based on the Cauchy–Schwarz bound. The simulation results show that with the tailored element position and phase shift optimization algorithms, the HE-IRS can achieve a competitive performance compared to that of the conventional IRS, but with much lower power consumption. Full article
(This article belongs to the Special Issue Design and Measurement of Millimeter-Wave Antennas)
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32 pages, 3694 KiB  
Article
Decoding Urban Traffic Pollution: Insights on Trends, Patterns, and Meteorological Influences for Policy Action in Bucharest, Romania
by Cristiana Tudor, Alexandra Horobet, Robert Sova, Lucian Belascu and Alma Pentescu
Atmosphere 2025, 16(8), 916; https://doi.org/10.3390/atmos16080916 - 29 Jul 2025
Viewed by 406
Abstract
Traffic-related pollutants remain a challenging global issue, with significant policy implications. Within the European Union, Romania has the highest yearly societal cost per capita due to air pollution, which kills 29,000 Romanians every year, whereas the health and economic costs are also significant. [...] Read more.
Traffic-related pollutants remain a challenging global issue, with significant policy implications. Within the European Union, Romania has the highest yearly societal cost per capita due to air pollution, which kills 29,000 Romanians every year, whereas the health and economic costs are also significant. In this context, municipal authorities in the country, particularly in high-density areas, should place a strong focus on mitigating air pollution. In particular, the capital city, Bucharest, ranks among the most congested cities in the world while registering the highest pollution index in Romania, with traffic pollution responsible for two-thirds of its air pollution. Consequently, studies that assess and model pollution trends are paramount to inform local policy-making processes and assist pollution-mitigation efforts. In this paper, a generalized additive modeling (GAM) framework is employed to model hourly concentrations of nitrogen dioxide (NO2), i.e., a relevant traffic-pollution proxy, at a busy urban traffic location in central Bucharest, Romania. All models are developed on a wide, fine-granularity dataset spanning January 2017–December 2022 and include extensive meteorological covariates. Model robustness is assured by switching between the generalized additive model (GAM) framework and the generalized additive mixed model (GAMM) framework when the residual autoregressive process needs to be specifically acknowledged. Results indicate that trend GAMs explain a large amount of the hourly variation in traffic pollution. Furthermore, meteorological factors contribute to increasing the models’ explanation power, with wind direction, relative humidity, and the interaction between wind speed and the atmospheric pressure emerging as important mitigators for NO2 concentrations in Bucharest. The results of this study can be valuable in assisting local authorities to take proactive measures for traffic pollution control in the capital city of Romania. Full article
(This article belongs to the Special Issue Sources Influencing Air Pollution and Their Control)
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20 pages, 5588 KiB  
Article
Rapid and Robust Generation of Homozygous Fluorescent Reporter Knock-In Cell Pools by CRISPR-Cas9
by Jicheng Yang, Fusheng Guo, Hui San Chin, Gao Bin Chen, Ziyan Zhang, Lewis Williams, Andrew J. Kueh, Pierce K. H. Chow, Marco J. Herold and Nai Yang Fu
Cells 2025, 14(15), 1165; https://doi.org/10.3390/cells14151165 - 29 Jul 2025
Viewed by 389
Abstract
Conventional methods for generating knock-out or knock-in mammalian cell models using CRISPR-Cas9 genome editing often require tedious single-cell clone selection and expansion. In this study, we develop and optimise rapid and robust strategies to engineer homozygous fluorescent reporter knock-in cell pools with precise [...] Read more.
Conventional methods for generating knock-out or knock-in mammalian cell models using CRISPR-Cas9 genome editing often require tedious single-cell clone selection and expansion. In this study, we develop and optimise rapid and robust strategies to engineer homozygous fluorescent reporter knock-in cell pools with precise genome editing, circumventing clonal variability inherent to traditional approaches. To reduce false-positive cells associated with random integration, we optimise the design of donor DNA by removing the start codon of the fluorescent reporter and incorporating a self-cleaving T2A peptide system. Using fluorescence-assisted cell sorting (FACS), we efficiently identify and isolate the desired homozygous fluorescent knock-in clones, establishing stable cell pools that preserve parental cell line heterogeneity and faithfully reflect endogenous transcriptional regulation of the target gene. We evaluate the knock-in efficiency and rate of undesired random integration in the electroporation method with either a dual-plasmid system (sgRNA and donor DNA in two separate vectors) or a single-plasmid system (sgRNA and donor DNA combined in one vector). We further demonstrate that coupling our single-plasmid construct with an integrase-deficient lentivirus vector (IDLV) packaging system efficiently generates fluorescent knock-in reporter cell pools, offering flexibility between electroporation and lentivirus transduction methods. Notably, compared to the electroporation methods, the IDLV system significantly minimises random integration. Moreover, the resulting reporter cell lines are compatible with most of the available genome-wide sgRNA libraries, enabling unbiased CRISPR screens to identify key transcriptional regulators of a gene of interest. Overall, our methodologies provide a powerful genetic tool for rapid and robust generation of fluorescent reporter knock-in cell pools with precise genome editing by CRISPR-Cas9 for various research purposes. Full article
(This article belongs to the Special Issue CRISPR-Based Genome Editing Approaches in Cancer Therapy)
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18 pages, 305 KiB  
Review
Causes of Childhood Cancer: A Review of Literature (2014–2021): Part 2—Pregnancy and Birth-Related Factors
by Rebecca T. Emeny, Angela M. Ricci, Linda Titus, Alexandra Morgan, Pamela J. Bagley, Heather B. Blunt, Mary E. Butow, Jennifer A. Alford-Teaster, Raymond R. Walston III and Judy R. Rees
Cancers 2025, 17(15), 2499; https://doi.org/10.3390/cancers17152499 - 29 Jul 2025
Viewed by 590
Abstract
Purpose: To review parental pre-pregnancy and pregnancy exposures in relation to pediatric cancer (diagnosis before age 20). Methods: We conducted literature searches using Ovid Medline and Scopus to find primary research studies, review articles, and meta-analyses published from 2014 to 17 March 2021. [...] Read more.
Purpose: To review parental pre-pregnancy and pregnancy exposures in relation to pediatric cancer (diagnosis before age 20). Methods: We conducted literature searches using Ovid Medline and Scopus to find primary research studies, review articles, and meta-analyses published from 2014 to 17 March 2021. Results: Strong evidence links increased risk of childhood cancer with maternal diabetes, age, and alcohol and coffee consumption during pregnancy. Both paternal and maternal cigarette smoking before and during pregnancy are associated with childhood cancers. Diethylstilbestrol (DES) exposure in utero has long been known to be causally associated with increased risk of vaginal/cervical cancers in adolescent girls. More recent evidence implicates in utero DES exposure to testicular cancer in young men and possible intergenerational effects on ovarian cancer in the granddaughters of women exposed to DES during pregnancy. There is strong evidence that childhood cancer risk is also associated with both high and very low birth weight and with gestational age. Evidence is also strong for the protective effects of maternal vitamin consumption and a healthy diet during pregnancy. Unlike early studies, those reviewed here show no association between in utero exposure to medical ionizing radiation, which may be explained by reductions over time in radiation doses, avoidance of radiation during pregnancy, and/or by inadequate statistical power to detect small increases in risk, rather than a lack of causal association. Evidence is mixed or conflicting for an association between childhood cancer and maternal obesity, birth order, cesarean/instrumental delivery, and prenatal exposure to diagnostic medical radiation. Evidence is weak or absent for associations between childhood cancer and multiple gestations or assisted reproductive therapies, as well as prenatal exposure to hormones other than DES, and medications. Full article
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