Search Results (2,152)

Hydroponic and soilless systems are increasingly adopted as low-cost, sustainable solutions for global food production, yet they remain highly susceptible to contamination by potential toxic elements (PTEs), particularly arsenic. While biochar is widely recognized as an effective amendment for mitigating PTE contamination in soil-based systems, its ability to alleviate PTE stress in hydroponic environments has been largely overlooked. The gap reveals a critical and underexplored frontier in controlled-environment agriculture, where extending biochar-based mitigation strategies could yield substantial benefits. Here, we evaluated whether Acacia auriculiformis wood biochar could alleviate arsenic (As) toxicity in lettuce (Lactuca sativa) grown in a continuous-flow hydroponic system. Using a completely randomized factorial design (arsenic species × dose × biochar) with three independent replicates per treatment, we tested biochar under 0.2 and 0.8 mg/L of As(III) and As(V). Arsenic significantly (p < 0.05) reduced lettuce growth, with As(III) being more toxic than As(V). Biochar significantly (p < 0.05) improved morphological traits (2.4–103%), cell membrane stability (5.5–12%), photosynthetic pigments (3–73%), and stress indicators proline (8–11%) and malondialdehyde (8–14%). Arsenic accumulated mainly in roots (1.7–2.63 mg/kg) and shoots (0.76–1.36 mg/kg), but biochar reduced accumulation by 28–47% in roots and 33–48% in shoots. Additionally, biochar enhanced nutrient uptake (K, P, Ca, Mg, B, Zn, Cu, S, Mn) at both arsenic levels. Overall, the results indicate that Acacia biochar can substantially reduce arsenic toxicity and improve plant physiological responses in continuous-flow hydroponics, highlighting its promise as a viable and scalable mitigation tool for safeguarding soilless food production systems against PTE contamination. Full article

Controlled environment agriculture (CEA), including hydroponics, vertical farming (VF), and soilless cultivation, is expanding rapidly as food production shifts toward resource-efficient and climate-resilient systems. However, conventional substrates such as rockwool, peat, coco coir, and perlite present limitations related to nutrient buffering, structural stability, and environmental sustainability. Biochar has emerged as a promising alternative substrate component due to its porous structure, surface functionality, and ability to modify root-zone conditions. This review synthesizes current knowledge on the role of biochar in controlled cultivation systems, focusing on its physicochemical properties, substrate interactions, and plant physiological responses. Biochar incorporation influences water retention, aeration, nutrient availability, and microbial activity within confined root environments, thereby improving root architecture, photosynthetic performance, crop quality, and plant uniformity. Applications across hydroponic, VF, and soilless cultivation systems demonstrate improved moisture regulation, nutrient buffering, and substrate stability. Biochar interactions with conventional media such as coco peat, perlite, and peat moss further highlight its role in engineered growing substrates. Despite these advantages, challenges remain, including feedstock variability, pH and electrical conductivity effects, lack of standardized specifications, and limited long-term performance data in recirculating systems. Emerging research areas such as engineered biochar, nano-biochar, microbial integration, and precision cultivation technologies offer opportunities to optimize biochar performance in controlled environments. Overall, biochar represents a versatile and sustainable substrate component for CEA, with potential to enhance crop productivity, substrate durability, and resource efficiency. Future research should focus on material standardization, system-specific optimization, and large-scale validation to support commercial adoption. Full article

To investigate the long-term water use efficiency (WUE) and ecological adaptation strategies of seven endangered Ormosia plant species, and to identify superior tree species with strong drought resistance, thereby providing a scientific basis for ecological restoration, represented the objectives of this research. This study focused on Ormosia semicastrata, Ormosia pinnata, Ormosia fordiana, Ormosia xylocarpa, Ormosia glaberrima, Ormosia emarginata and Ormosia henryi. Using these seven Ormosia species as subjects of study, and the commonly planted afforestation species Michelia macclurei and Castanopsis hystrix as controls, we determined long-term water-use efficiency by measuring stable carbon isotope (δ¹³C) values of the leaves. This was combined with growth indices and chlorophyll content; a systematic analysis was conducted of interspecific differences and similarities with the afforestation species, to reveal the ecological adaptability and survival potential of Ormosia plants in arid environments. Methodologically, leaf carbon isotope (δ¹³C) values were measured for each tree species to estimate long-term water use efficiency, whilst growth indices and chlorophyll content were also determined to compare differences between Ormosia plants and the control species. The results indicate that the leaf carbon isotope (δ¹³C) values of the seven Ormosia species ranged from −31.88‰ to −29.03‰. Among these, O. xylocarpa, O. pinnata and O. emarginata exhibited higher leaf carbon isotope (δ¹³C) values and water use efficiency, suggesting greater water utilisation capacity and drought tolerance; whereas O. fordiana and O. glaberrima exhibited lower values for both parameters. Chlorophyll analysis revealed that the total chlorophyll content of O. pinnata and O. fordiana was significantly higher than that of Michelia macclurei and Castanopsis hystrix, and the chlorophyll a to chlorophyll b ratio of all Ormosia species was higher than that of the afforestation tree species, suggesting greater photosynthetic capacity and adaptability to high-light environments. Furthermore, leaf carbon isotope (δ¹³C) values showed a highly significant positive correlation with water use efficiency, validating the reliability of carbon isotopes in assessing long-term water use efficiency in plants. Taking all indicators into account, O. xylocarpa and O. pinnata demonstrated good root development and efficient allocation of photosynthetic pigments whilst maintaining high water use efficiency; they are therefore considered excellent tree species that combine drought resistance with growth potential. By integrating growth, photosynthetic and water-use characteristics, this study has clarified the water-adaptation strategies of different Ormosia species, providing a scientific basis for selecting drought-tolerant Ormosia species and for ecological restoration. Full article

The present study investigated the effects of different soil substrates (mixtures, based on a mica schist-derived soil), on the plant growth, soil chemical properties, nutritional status and photosynthetic parameters of chestnut (Castanea sativa) seedlings. The aim was to produce robust seedlings, suitable for plantations or reforestation of forest ecosystems, while also provide the necessary nutrient input during the early stages of development. A pot experiment was conducted comprising four treatments: (i) CONTROL, consisting of soil derived from mica schist weathering (MS), (ii) MS with inorganic fertilization (MS-FER), (iii) MS amended with forest floor, derived from evergreen broad-leaved trees (MS-EFF); and (iv) MS amended with forest floor, derived from chestnut trees (MS-CFF). Regarding seedlings’ growth, both types of forest floor exerted a positive effect similar to that observed after inorganic fertilization (MS-FER), while all treatments maintained a satisfactory and stable photosynthetic performance; however, an enhancement in leaf chlorophyll content (CCI) was specifically observed under inorganic fertilization. Except Olsen P and exchangeable Mg, which were significantly higher in the MS-FER treatment, all the other soil nutrients were higher either in the MS-EFF, or in the MS-CFF treatments. Overall, both types of forest floor proved effective as organic amendments, suggesting that they could serve as an alternative or complement to inorganic fertilization, potentially reducing fertilizer inputs in chestnut seedling production. Full article

Due to climate change and urbanization, green roofs are vital for urban climate resilience. However, there is currently no consensus on the photosynthetic carbon uptake capacity of green roof plants in cities. This study investigated and compared nine common green roof plant species in Xiamen, a typical coastal city in southeastern China. Their photosynthetic parameters were measured across all four seasons to evaluate photosynthetic carbon uptake capacity, and the differences among the nine plant species were evaluated. Results show: (1) Significant interspecific differences exist. Ligustrum japonicum (Lj) had the highest seasonal average daily carbon uptake per unit leaf area (8.86 g m⁻² d⁻¹) and per unit area (93.10 g m⁻² d⁻¹), approximately 9 times that of the lowest-performing species per unit leaf area (Pedilanthus tithymaloides) and 11 times that of the lowest-performing species per unit area (Tradescantia spathacea). (2) The variation in carbon uptake capacity among different green roof plant species followed a pattern consistent with that of their net photosynthetic rate and leaf area index. (3) Overall, the carbon uptake capacity of the nine plant species exhibited a trend of trees > shrubs > herbs. In summary, Lj demonstrated the highest photosynthetic carbon uptake capacity among the nine species examined, suggesting its potential as a promising species for enhancing photosynthetic carbon uptake on green roofs in southeastern coastal cities. Full article

Origanum L. (Lamiaceae) is a commercially important medicinal and aromatic plant genus worldwide. Endophytic bacterial communities are recognized for promoting plant growth and physiology, although their interactions with host metabolism remain insufficiently understood. In this work, an in vitro model of axenic Origanum heracleoticum plants was established to investigate the relationship between endophytic bacteria and their tissue of origin. Specifically, we evaluated the adaptation of two strains, Bacillus sp. OHL2 and Pseudomonas sp. OHS18, and the potential role of Bacillus sp. OHL2 in modulating plant physiology and secondary metabolism. Bacterial inoculation and re-isolation highlighted niche-specific adaptation and possible co-evolution within the host, suggesting an active role of the plant in regulating bacterial colonization within the endosphere. Inoculation with Bacillus sp. OHL2 significantly enhanced photosynthetic rate, leaf area, dry weight, and chlorophyll content. No substantial overall changes in secondary metabolism were detected. Rosmarinic acid was the predominant phenolic, while monoterpenes dominated, with carvacrol dominant. A significant tissue-by-inoculation interaction was observed for α-humulene, which decreased in leaves of inoculated plants. Overall, the in vitro system provides a valuable platform to study plant–endophyte interactions and bacterial mechanisms underlying the stimulation of plant growth and metabolic responses. Full article

To address the issues of low water and fertilizer use efficiency and limited yield potential in traditional winter wheat cultivation in Henan Province, and to determine the optimal drip irrigation frequency for wide-row precision sowing under a constant total irrigation amount, this study was conducted based on a field experiment in Zhengzhou, Henan, during the 2024–2025 season. Four treatments were set up: border irrigation with wide-row precision sowing (QK40), and single-drip irrigation events of 25 mm (DK25, high frequency), 40 mm (DK40, medium frequency), and 55 mm (DK55, low frequency). The effects of drip irrigation frequency on photosynthetic accumulation after anthesis (AUC), source–sink coordination index (SSCI), and the synergy among water, grain, and quality in wide-row precision-sown winter wheat were quantitatively analyzed. The results showed that DK25 significantly delayed leaf senescence and extended the green leaf functional period by 9 days by stabilizing moisture in the 0–40 cm root zone. Post-anthesis photosynthetic accumulation increased by 23.39% and was highly significantly positively correlated with yield. The leaf area index at the heading stage increased by 23.54%, and the source–sink coordination index (SSCI) improved by 45.1%. Over the whole growth period, water consumption was reduced by 10.38%, water use efficiency increased by 23.5%, and yield increased by 8.9%, while grain quality remained stable. Entropy Weight-TOPSIS evaluation showed that DK25 performed the best. This study can provide a cultivation pattern and technical parameters for water-saving, high-yield, and high-quality wide-row precision-sown winter wheat in the Huang-Huai-Hai Plain. Full article

A sustainable method of improving soil fertility and developing climate-resilient cropping systems is vermicomposting agricultural waste. This study hypothesized that vermicompost derived from mixed organic agricultural wastes would significantly improve soil fertility, crop productivity, and drought resilience compared to single-substrate treatments and the unamended control. The effects of vermicompost generated from mixed organic wastes using Eisenia fetida on soil quality, crop performance, and drought resilience of lettuce (Lactuca sativa, Eden variety) were evaluated in this study using a randomized complete block design. Crop performance indicators included germination, growth characteristics, biomass, SPAD chlorophyll content, and yield, while soil physicochemical properties, including pH, organic carbon, total nitrogen, available phosphorus, exchangeable potassium, electrical conductivity (EC), and cation exchange capacity (CEC), were assessed both before and after amendment application. The effects of drought stress were evaluated using leaf surface temperature, wilting score, recovery time, and survival rate. The results demonstrated that vermicompost application significantly improved soil fertility and crop performance relative to the control treatment (p < 0.05). The best-performing treatment (T2) increased soil organic carbon by approximately 22–28%, total nitrogen by 18–24%, available phosphorus by 20–27%, and exchangeable potassium by 16–21% compared with the control. Fresh biomass increased by approximately 14–17%, marketable yield improved by 16–24%, and SPAD chlorophyll values increased by nearly 20%, indicating enhanced photosynthetic efficiency and nutrient uptake. T2 showed the most resilience under drought stress, with ~94.9% survival rate, reduced wilting severity, shortened recovery time and sustained stable leaf temperature (~27.8 °C), whereas low-performing treatments and the control recorded survival rates of only ~70–78%. Mixed organic waste substrates consistently outperformed single-substrate treatments, demonstrating synergistic effects on nutrient cycling, microbial activity, soil structural quality, and drought tolerance. These findings provide quantitative evidence that vermicomposting can simultaneously enhance soil fertility, crop productivity, and drought resilience, highlighting its strong potential as a scalable climate-smart strategy for sustainable agriculture, circular bioeconomy development, and organic waste valorization in smallholder farming systems. Full article

WPR (WEB1/PMI2-related) genes play a crucial role in regulating chloroplast movement and leaf coloration in plants. Previous studies have shown that these genes are implicated in leaf yellowing, both in Arabidopsis thaliana and in Paulownia fortunei following infection with Paulownia witches’ broom. To investigate the functions of the ZjWPR genes in jujube, bioinformatics methods were employed to identify the ZjWPR gene family in jujube, analyze their protein physicochemical properties, gene structure, evolutionary relationships, and cis-acting elements in this study. The results revealed that the ZjWPR gene family in jujube comprised 10 members. Phylogenetic analysis showed that WPR genes were divided into two classes, with ZjWPR genes distributed across three subgroups within Class II. Conserved motif analysis indicated that motif 2, motif 3, motif 7, and motif 8 were the most highly conserved and most genes exhibited similar structures. Cis-element analysis in their promoter suggested that ZjWPR genes were regulated by multiple hormones and were associated with stress responses such as low temperature and drought. Moreover, all ZjWPR genes contained light-responsive elements. Expression analysis of the ZjWPR gene family under Jujube Witches’ Broom (JWB) stress showed that ZjWPR4 and ZjWPR5 were significantly up-regulated in JWB-susceptible jujube cultivars following phytoplasma infection, whereas no significant changes were detected in JWB-resistant cultivars. Additionally, the expression levels of ZjWPR2, ZjWPR3, and ZjWPR6 were also altered in response to infection, suggesting their potential involvement in the response to JWB stress and the associated leaf chlorosis process. Moreover, transient overexpression of ZjWPR4 and ZjWPR5 in sour jujube leaves led to significant reductions, in critical photosynthetic parameters, including Fv/Fm, Fq′/Fm′, and ETR compared with WT, thereby reinforcing their functional contribution to JWB-associated leaf yellowing. This study provides valuable insights for further functional characterization of the ZjWPR gene family in mediating JWB-induced leaf yellowing and related metabolic pathways. Full article

Usnic acid (UA) is a secondary metabolite produced by lichens that has attracted interest because of its antimicrobial, photoprotective, and antioxidant properties, suggesting its potential use as a biostimulant in agriculture. However, its evaluation in agricultural crops is limited. In the present study, we evaluated the effect of applying (+) UA on enzymatic and non-enzymatic antioxidant systems, photosynthetic pigments, photosynthetic enzyme activity, and markers of oxidative stress in “Albion” strawberry plants. The plants were grown in a peat moss:perlite substrate (1:1, v/v) and cultivated under tunnel greenhouse conditions using a nutrient solution applied via fertigation. (+) UA was applied at 400 µg/mL via three routes (foliar, drench, and a combination of foliar and drench) on three occasions. Leaf tissue was collected 117 days after transplantation, and the biochemical parameters were quantified. (+) UA increased the activity of glutathione peroxidase (GPX) (53% via foliar-drench) and catalase (CAT) by 73.5% (via drench), and reduced glutathione (GSH) content by 58% (via foliar). β-carbonic anhydrase (βCA) activity increased by 415% and 384% (foliar and foliar-drench, respectively). Likewise, Ribulose 1,5-bisphosphate carboxylase-oxygenase (RuBisCO) activity increased by 58.23% (drench) and phosphoenolpyruvate carboxylase (PEPC) by 25% and 46% (foliar and drench), suggesting positive effects on the processes associated with CO₂ assimilation and transport. In contrast, no significant changes were observed in the levels of hydrogen peroxide (H₂O₂), malondialdehyde (MDA), or proline, indicating the absence of oxidative stress. These findings suggest that (+) UA modulates the enzymatic antioxidant system, promoting favorable physilogical responses without inducing oxidative stress. The use of (+) UA has been proposed as a potential promoter of metabolism in agricultural crops. In addition, new avenues of research are being explored to investigate the role in modulating antioxidant responses under biotic and abiotic stress conditions. Full article

Ionic liquids (ILs) are widely used chemical compounds that may pose potential risks to the environment. In the present study, the effects of 1-ethyl-3-methylimidazolium chloride (EMIMCl) on growth, photosynthetic performance, and oxidative stress in maize (Zea mays L.) seedlings were evaluated, and the role of exogenous L-ascorbic acid (AsA) in modulating plant responses to this stress was investigated. Plants were cultivated in soil contaminated with EMIMCl at concentrations ranging from 1 to 1000 mg·kg⁻¹ of soil dry weight (DW) and treated with AsA at concentrations of 0.5–2 mM. EMIMCl significantly inhibited plant growth, reduced photosynthetic pigment content, and impaired chlorophyll fluorescence parameters, accompanied by increased hydrogen peroxide (H₂O₂) and malondialdehyde equivalents (MDA) levels, indicating the induction of oxidative stress. Moderate doses of AsA partially alleviated EMIMCl-induced toxicity, whereas higher AsA concentrations under severe EMIMCl contamination intensified stress symptoms. These findings demonstrate a dose-dependent and biphasic role of AsA in maize responses to EMIMCl-induced stress. Full article

Rice (Oryza sativa L.) is one of the world’s major staple foods. However, its production is severely constrained by rice blast disease, caused by Magnaporthe oryzae, which leads to substantial yield losses. Conventional management relies on fungicides and chemical treatments; however, these methods raise concerns regarding the development of pathogen resistance and potential environmental impacts. This study evaluated carrageenan from Hypnea musciformis, collected from the coast of Saint Martin (92°19′21.28″ E and 20°37′38.12″ N), located in the Bay of Bengal, Bangladesh, as a natural plant growth promoter as well as a biocontrol agent. Carrageenan was characterized by high sulfate (19–35%) and galactose (12–18%) contents, with FT-IR confirming characteristic κ-carrageenan functional groups. Application of 15% carrageenan significantly increased the germination of seed (27%), seedling vigor (93%), shoot and root lengths (54% and 47%), and biomass compared with untreated controls. Carrageenan markedly suppressed M. oryzae, inhibiting mycelial growth (83%), reducing conidiogenesis and conidial germination, and decreasing lesion length in detached leaves and potted plants. Treated rice seedlings exhibited improved soluble sugars, photosynthetic pigments, proline, phenolic and flavonoid contents, and enhanced antioxidant enzyme activities such as CAT (catalase) and POD (peroxidase), while lowering oxidative stress markers such as H₂O₂ and MDA (malondialdehyde). These results demonstrate that carrageenan from H. musciformis enhances rice growth and elicits defense responses against rice blast, offering a sustainable and environmentally friendly alternative to chemical-based fungicides for integrated M. oryzae management. Full article

Trehalose-6-phosphate (T6P) is involved in the regulation of plant growth and stress-related responses; however, its potential role in post-drought recovery remains unclear in legume crops. This study evaluated whether foliar application of native T6P could improve growth, yield formation, and physiological recovery in adzuki bean after pre-flowering drought stress. We aimed to propose a model for the production and drought tolerance regulation in adzuki bean. A three-year field experiment was conducted by applying T6P at both the budding stage and the initial pod stage. The plant architecture, yield, photosynthetic characteristics, defensive enzyme activity, sugar metabolism and hormone changes were evaluated. The results indicated that T6P significantly increased root, stem and leaf parameters in adzuki bean it also increased all yield traits except for the main stem branches and sections during post-drought recovery. Indeed, the yield per block of 5μM and 10μM of T6P application during post-drought recovery increased by 20.66% and 31.60% on average compared to the control under well-watered conditions and by 47.68% and 60.20% under drought stress during the three experimental years. Foliar T6P treatment was associated with higher antioxidant enzyme activities and related gene expression, lower ROS accumulation and proline content, and changes in sugar- and hormone-related traits during post-drought recovery. Therefore, foliar application of T6P at the budding and initial pod stages improved growth, yield formation, and post-drought recovery in adzuki bean. The result provide a novel strategy for T6P application for coordinating the high production potential and crop resilience. Full article

Salt stress severely restricts plant growth and reduces crop yield. Strigolactones (SLs) are carotenoid-derived phytohormones involved in the regulation of plant salt tolerance. Salt stress can modulate the expression of SL biosynthetic and signaling genes, thereby affecting SL accumulation and signaling responses. SLs also interact with abscisic acid (ABA), reactive oxygen species (ROS), and other signaling molecules to coordinate downstream stress responses. At the physiological level, SLs alleviate salt stress by maintaining Na⁺/K⁺ homeostasis, enhancing osmotic adjustment and antioxidant defense, and reducing damage to the photosynthetic system. In addition, SLs can enhance plant resource acquisition and adaptive capacity under salt stress by regulating root architecture and promoting hyphal branching of arbuscular mycorrhizal fungi (AMF). This review focuses on SL-mediated regulation of plant salt tolerance at the molecular and physiological levels and further summarizes exogenous SL application strategies for alleviating salt stress, as well as research progress on key genes in the SL pathway for the genetic improvement of salt tolerance. Clarifying the potential of SLs in regulating plant responses to salt stress could provide new insights into sustainable crop production in saline-alkali environments. Full article

Bisphenol A (BPA) is a common plastic additive found in wastewater (WW) due to its extensive use in industrial and consumer products. As a known endocrine disruptor, BPA poses serious ecological and human health risks, yet its removal remains inefficient in conventional WW treatment plants. This novel study investigates the impact of high environmental BPA concentrations (5 mg/L and 25 mg/L) on Chlorella vulgaris in a WW-mimicking environment, assessing microalgal growth, metabolic activity, nutrient removal, and BPA degradation. Exposure to BPA led to a significant reduction in esterase activity and an increase in intracellular reactive oxygen species (ROS) levels, indicating cellular oxidative stress and metabolic disruption. Despite these effects, C. vulgaris maintained stable photosynthetic pigment levels, demonstrating a resilient photosynthetic function. The bioremediation potential of C. vulgaris was also compromised, with nitrogen and phosphorus removal efficiencies decreasing by up to 38% and 34% in the presence of 5 and 25 mg BPA/L, respectively. Nevertheless, the microalga exhibited the ability to degrade BPA, with removal efficiencies of 34% for 5 mg/L and 21% for 25 mg/L after 168 h, while abiotic degradation was minimal. These findings confirm the potential of C. vulgaris as a promising sustainable approach for BPA bioremediation but also highlight critical challenges at high contaminant concentrations. Full article