Search Results (17,916)

The continuous emergence of SARS-CoV-2 variants, especially the Omicron strain with its heightened transmissibility, has posed ongoing challenges to the efficacy of existing vaccine and drug regimens. This situation highlights the pressing demand for antiviral drugs employing novel mechanisms of action. Pentacyclic triterpenoids (PTs), a structurally varied group of compounds derived from plants, exhibit both antiviral and anti-inflammatory activities, making them attractive candidates for further therapeutic development. These natural products, along with their saponin derivatives, show broad-spectrum inhibitory effects against multiple SARS-CoV-2 variants (from Alpha to Omicron) via interactions with multiple targets, such as the spike protein, main protease (Mpro), RNA-dependent RNA polymerase (RdRp), and inflammatory signaling pathways. This review consolidates recent findings on PTs and their saponins, emphasizing their influence on the key structural features required for inhibiting viral attachment, membrane fusion, reverse transcription, and protease function. We systematically summarized the structure–activity relationships and their antiviral results of PTs based on different target proteins in existing studies. Furthermore, this work points toward new strategies for designing multi-target PT-based inhibitors with improved efficacy against Omicron and future variants. Full article

Chloroplasts are key organelles in plants that carry out photosynthesis, convert light energy into chemical energy, and synthesize organic compounds. In this study, a stably heritable chlorophyll-deficient mutant was screened from the ethyl methanesulfonate-induced mutation library of Wuyunjing 21 (WYJ21). This mutant was designated as chlorophyll deficient 6 (cde6). The cde6 mutant exhibits a low chlorophyll content, photosynthetic defects, an impaired chloroplast structure, a significant reduction in the number of stacked thylakoid layers, and a yellow-green leaf phenotype in the early tillering stage. Through MutMap analysis, it was found that the cde6 mutant harbors a single-base mutation (T→A) in the LOC_Os07g38300 gene. This mutation results in an amino acid substitution from valine (Val) to aspartic acid (Asp) in the encoded protein, thereby affecting the protein’s structure and function. The mutation of CDE6 leads to decreased expression of genes related to chloroplast development and chlorophyll biosynthesis. Further studies revealed that the CDE6, a potential chloroplast ribosome recycle factor, leads to high temperature sensitivity in rice when mutated. As high-temperature stress is a primary constraint to global rice productivity, the identification of CDE6 provides a genetic target for improving thermotolerance. In conclusion, these findings demonstrate that CDE6 plays a crucial role in chloroplast biogenesis and provide new insights into its regulatory function in high-temperature tolerance. Full article

Some Sacoglossa sea slugs are capable of stealing and maintaining functional intracellular chloroplasts—kleptoplasts—from their macroalgal prey for periods of up to several months, a process known as kleptoplasty. Although the cultivation of these marine invertebrates under laboratory conditions is crucial for research in various fields (e.g., endosymbiosis, animal physiology, discovery of new marine natural products), rearing protocols are scarce. This study presents a standardized protocol for the laboratory rearing of large numbers of the sacoglossan tropical sea slug Elysia crispata. The detailed protocol successfully facilitated embryonic development, larval metamorphosis, and juvenile-to-adult transition, allowing the rearing of multiple generations. Two groups, characterized by acquiring different kleptoplasts, were obtained by feeding the sea slugs with two different prey macroalgae: Bryopsis sp. and Acetabularia acetabulum. Usually referred to as lettuce sea slug among marine aquarium hobbyists, E. crispata is a highly valued organism for its striking appearance and ability to control nuisance algal growth in reef aquariums. This protocol allows experimental reproducibility and access to specimens under different development stages, potentially boosting research on kleptoplasty while also contributing to reducing the impact of the marine aquarium trade on natural populations. Full article

In recent years, the application of augmented reality digital technology in brands has transformed the way consumers interact with brands. This study focuses on the impact of augmented reality (AR) technology on consumption behavior and brand communication related to intangible cultural heritage products, integrating the TAM and UTAUT2 theories to construct a research model. This study employed a time–location sampling method, utilizing SPSS and AMOS software for data analysis based on valid questionnaires completed by 305 AR-experiencing consumers in Changsha City, Hunan Province. Results indicate that the presence and novelty of AR technology significantly and positively influence consumers’ attitudes toward using AR technology, which in turn affects their purchase intent, social media sharing behavior, and brand attitudes. The study confirms that emotional factors and consumer perceptions play a guiding and decisive role in the new consumption reality enabled by AR technology. These research findings have practical significance and value for ICH brand building and AR marketing, demonstrating that AR is an effective means to enhance the visibility and influence of the ICH brand. They inject new vitality into promoting more sustainable ICH protection and popularization, as well as the development of the digital creative industry. Full article

Most animal welfare (AW) assessment protocols have been developed for intensive production systems and European cattle, raising concerns about their applicability in the tropics. To compare the results obtained by using the welfare quality (WQ) assessment for fattening cattle in the dry tropics, relevant modifications were implemented in 20 cattle production units (PUs) during the dry (DS) and rainy (RS) seasons. Regarding the principle of good feeding, only during the RS, between 20% and 25% of the farms maintained their animals in the acceptable and good categories, compared to the DS, where all PUs were classified as unacceptable (p < 0.04). Under the “Appropriate Behavior” principle, only 15% and 60% of the PUs maintained their animals at good and acceptable levels, respectively, in the RS, but not in the DS (p < 0.001). Conversely, during the DS, better scores were obtained for the measures and criteria in the Good housing group, with 45%, 50%, and 5% of PU classified as acceptable, good, and excellent, respectively, while for the RS, only 15%, 30%, and 5% reached these levels (p < 0.01). Meanwhile, under the “Good Health” principle, better animal health scores were observed during the RS, with 20%, 30%, and 50% of farms classified as acceptable, good, and excellent, compared to the DS, where only 70% and 10% of farms maintained their animals at good and excellent levels (p < 0.01). It is concluded that better animal welfare (AW) indicators were recorded during the RS, and the adjustments we applied to the conventional WQ protocol comprised a modification for the criterion that included the prolonged absence of thirst as well as adding six new indicators (measures) to the principles of housing, health, and behavior, which are considered essential for evaluating AW in cattle that are managed under extensive conditions by season. Full article

The Extended Digital Narrative (XDN) research project explores how experimental creative practice with emerging technologies generates critical insights into algorithmic narrativity—the intersection of human narrative understanding and computational data processing. This article presents five case studies demonstrating that direct engagement with AI and Extended Reality platforms is essential for humanities research on new genres of digital storytelling. Lina Harder’s Hedy Lamar Chatbot examines how generative AI chatbots construct historical personas, revealing biases in training data and platform constraints. Scott Rettberg’s Republicans in Love investigates text-to-image generation as a writing environment for political satire, documenting rapid changes in AI aesthetics and content moderation. David Jhave Johnston’s Messages to Humanity demonstrates how Runway’s Act-One enables solo filmmaking, collapsing traditional production hierarchies. Haoyuan Tang’s video game project reframes LLM integration by prioritizing player actions over dialogue, challenging assumptions about AI’s role in interactive narratives. Sérgio Galvão Roxo’s Her Name Was Gisberta employs Virtual Reality for social education against transphobia, utilizing perspective-taking techniques for empathy development. These projects demonstrate that practice-based research is not merely artistic production but a vital methodology for understanding how AI and XR platforms shape—and are shaped by—human narrative capacities. Full article

Whole-genome sequencing is a powerful approach for exploring genomic diversity in livestock species. Chickens (Gallus gallus) are an important food source worldwide, and in Taiwan, poultry production contributes substantially to the livestock industry. Taiwan’s commercial red- and black-feathered country chickens dominate this category and play a crucial role in local poultry production. However, fundamental genomic information on their population structure remains limited. To address this gap, this study generated whole-genome sequencing data from red-feathered country chickens originating from four major breeding farms. Genetic diversity analyses revealed uniformly low genetic diversity across all farms. Runs of homozygosity (ROH) analyses indicated predominantly historical inbreeding, with farm-specific differences in recent inbreeding patterns. Population structure analyses revealed clear clustering of individuals according to farm origin, indicating distinct line structures among breeding farms. These results provide the first comprehensive genomic overview of Taiwan’s commercial red-feather country chickens and offer valuable reference information for future breeding strategies and the development of new lines. Full article

Background: Advanced glycation end products (AGEs) play a pivotal role in various human pathologies, including aging and metabolic diseases, and their formation may have significant physiological consequences for human health. Fructoselysine (FL) is an intermediate in the formation of AGEs, and its accumulation has been associated with detrimental health effects. Although several chromatographic methods have been developed for AGEs detection and quantification, no mass spectrometry-based approach has previously been established to quantify FL in different human biological matrices. Methods: In this study, we present a novel UHPLC-HRMS/MS method for the identification and quantification of this compound in various biological matrices, including plasma, feces, and urine. Results: The method demonstrates excellent linearity, accuracy, and precision, with limit of detection (LOD) of 0.02 µM and limit of quantification (LOQ) of 0.06 µM. Recovery rates ranged from 95% to 109% and intra- and inter-day relative standard deviations (RSDs) were below 10%, indicating robust analytical performance. The validated method was successfully applied to quantify FL in plasma, feces, and urine samples from healthy individuals. Additionally, given the known association between AGEs and diabetes, we analyzed a small cohort of prediabetic patients and observed elevated circulating levels of FL compared to healthy controls. Conclusions: This study introduces a sensitive and reliable method for the specific detection and quantification of FL in biological samples and provides new insights into early molecular changes associated with prediabetic condition to improve early diagnosis in aging related diseases. Full article

As the global climate discourse shifts from mitigation to achieving net-negative emissions, there is a critical need for replicable, real-world models of climate-positive development at a regional scale, particularly in the Global South. This study addresses this gap by conducting a detailed greenhouse gas (GHG) inventory of four diverse provinces in Thailand and analyzing the results through the newly proposed Climate-Positive Pathways Framework (CPPF). Our findings reveal that all four provinces function as significant net-negative GHG sinks. They achieve this status through three distinct archetypes: a Conservation-Dependent pathway, an Agricultural Frontier pathway, and a novel Agro-Sink pathway. Most significantly, in the Agro-Sink model, we find that in specific economic contexts, managed agricultural landscapes can surpass natural forests as the primary driver of regional carbon removal. This typology provides a new, landscape-scale paradigm for cleaner production, proposing these three archetypes as transferable, evidence-based models for regional policymakers. This underscores that effective climate action requires context-specific regional planning that strategically leverages both natural and agricultural capital. Full article

This study developed a bio-based fluid loss reducer based on itaconic acid-grafted corn starch (IACS) for water-based drilling fluid systems. The product was synthesized through free radical graft copolymerization and characterized by FTIR, TGA, and SEM. In bentonite-based mud systems, IACS demonstrated excellent filtration control performance significantly superior to that of conventional fluid loss reducers such as PAM, CMC, and PAC. IACS exhibited outstanding temperature resistance, salt tolerance, and calcium contamination resistance. Particle size analysis revealed that IACS effectively dispersed bentonite particles to the nanoscale at elevated temperatures, preventing thermal aggregation. Mechanistic studies indicated that carboxyl groups introduced by the grafting reaction endowed IACS with strong adsorption capacity and hydration ability, forming a dense polymer network layer on clay particle surfaces. Environmental evaluation confirmed that IACS possessed moderate biodegradability and extremely low toxicity, meeting green drilling fluid additive requirements. This study provides new insights for developing high-performance, environmentally friendly fluid loss reducers. Full article

This study addresses the critical need for advanced biomedical materials that possess both potent antimicrobial properties and high biocompatibility to prevent device-related infections and promote healing. To this end, we demonstrate the successful development and comprehensive characterization of functional composite materials based on a photopolymerizable acrylate resin modified with laser-ablated copper nanoparticles (Cu NPs). The synthesized Cu NPs exhibited a monomodal size distribution with a peak at 47 nm, a high zeta potential of −33 mV, and a spherical morphology. Incorporation of Cu NPs into the polymer matrix via Masked Stereolithography (MSLA) enabled the fabrication of complex structures that maintained high surface quality and optical transparency after polishing. Modification of photopolymer resin with Cu NPs significantly increased the strength of the resulting products and caused dose-dependent formation of reactive oxygen species (ROS). The resulting composite materials exhibited strong antibacterial activity against E. coli. Crucially, despite their potent antimicrobial efficacy, the materials showed no cytotoxicity towards human fibroblast cultures. These results highlight the potential of these composites for a new generation of biomedical applications, such as implantable devices and wound coatings, which combine programmable antimicrobial activity with high biocompatibility. Full article

This paper synthesizes lessons learned from drilling a CO₂-storage stratigraphic well in the San Juan Basin (New Mexico, USA) to clarify drivers of operational incidents and to inform future well planning. A literature review of regional drilling problems was combined with pre-drill engineering based on offset-well history and a geomechanical model, including casing, cementing, and hydraulics designs developed in commercial software; these designs were compared with field execution to extract incident-specific lessons. The most frequent problems observed are lost circulation, stuck pipe, and poor control of drilling parameters, consistent with complex lithology and reservoir pressure depletion that reduces fracture pressure below anticipated values. Based on the lessons learned, three mitigations are proposed as follows: (1) update the geomechanical model with the latest pore, fracture pressure estimates; (2) apply underbalanced drilling using nitrified mud by injecting nitrogen through a parasite string while drilling intermediate and production sections; and (3) maintain operating limits (weight on bit < 44.5 kN, top-drive rotation < 45 rpm, and pump rate < 1.32 m³/min) to improve fluid returns through low-fracture-pressure intervals. Simulation results support the applicability of the proposed solutions. Full article

Compared to traditional marine productivity, marine new-quality productivity (MNQP) is composed of advanced productive forces driven by the deepening application of new technologies, is characterized by the rapid emergence of new industries, new business models, and new modes of operation, and is marked by a substantial increase in total factor productivity in the marine economy. It has, therefore, become a new engine and pathway for China’s development into a maritime power. The main research approaches and conclusions of this paper are as follows: ① Using a combined order relation analysis method–Entropy Weight Method (G1-EWM) weighting method that integrates subjective and objective factors, we measured the development level of China’s MNQP from 2006 to 2021 across two dimensions: “factor structure” and “quality and efficiency”. The findings indicate that China’s MNQP is developing robustly and still holds considerable potential for improvement. ② Utilizing Gaussian Kernel Density Estimation and Spatial Markov Chain analysis to examine the dynamic evolution of China’s MNQP, the study identifies breaking the low-end lock-in of MNQP as crucial for accelerating balanced development. Spatial imbalances in China’s MNQP may exist both at the national level and within the three major marine economic zones. ③ To further examine potential spatial imbalances, Dagum Gini decomposition was employed to assess regional disparities in China’s MNQP. The DER polarization index and EGR polarization index were used to analyze spatial polarization levels, revealing an intensifying spatial imbalance in China’s MNQP. ④ Finally, geographic detectors were employed to identify the factors influencing spatial imbalances in China’s MNQP. Results indicate that these imbalances result from the combined effects of multiple factors, with marine economic development emerging as the core determinant exerting a dominant influence. The core conclusions of this study provide theoretical support and practical evidence for advancing the enhancement of China’s MNQP, thereby contributing to the realization of the goal of building a maritime power. Full article

Background: Advanced glycation end-products (AGEs) are formed and deposited in tissues, contributing to various disorders, including diabetes, other metabolic diseases, and aging. A new epitope, AGE10, was identified in human and animal tissues using a monoclonal antibody raised against synthetic melibiose-derived glycation end-products (MAGE), which were synthesized under anhydrous conditions with bovine serum albumin or myoglobin. The biology of the AGE10 epitope, particularly its role in diseases and in cancer tissues, is not well understood. Methods: The study was aimed at investigating the immunohistochemical recognition of AGE10 with the MoAb-anti-MAGE antibody. Results: Data obtained show that AGE10 is recognized in striated muscles but not in tumors of muscular origin. AGE10 is also stained in both normal and cancerous salivary glands and in adenomas of the large intestine. The staining is cytoplasmic. Discussion: Our approach may provide a methodology for cell biology research; AGE10 may be related to an advanced lipoxidation end-product; further investigation of MAGE may clarify disease mechanisms, support the development of novel therapeutic strategies. Conclusions: The key finding is that antibodies recognize mainly the epitope in epithelial and some mesenchymal tissues. Thus, the potential for AGE10 as a diagnostic marker is limited. The implications concern the biology of this epitope, the unique tissue distribution, and a role in cellular metabolism. Full article

This study evaluates the feasibility of establishing a new strawberry cultivation zone in the Region of Florina, Northern Greece, as a strategy to support rural revitalization and agricultural diversification. Day-neutral strawberry genotypes were cultivated under net-house conditions at the University of Western Macedonia and assessed for physiological traits (SPAD index, chlorophyll fluorescence) and fruit quality (weight, color, firmness, °Brix, titratable acidity); while postharvest behavior was evaluated after seven days of cold storage. Statistical analysis identified genotypes with superior physiological performance and storability. Preliminary economic analysis suggests that their adoption could increase growers’ income by 20–30% compared to conventional varieties. The findings support the development of a strawberry production zone in Florina, with broader implications for sustainable agricultural intensification and rural development in underutilized European regions. Full article