Search Results (182)

This study sought to quantify and characterize diverse rootstock scion interactions in cacao around graft compatibility, disease resistance, nutrient use efficiency, vigor traits, and translocation of nonstructural carbohydrates. In total, 106 grafts were performed with three scion cultivars (Matina 1/6, Criollo 22, Pound 7) and nine diverse open-pollinated seedling populations (BYNC, EQX 3348, GNV 360, IMC 14, PA 107, SCA 6, T 294, T 384, T 484). We found evidence for both local and translocated graft incompatibility. Cross sections and Micro-XCT imaging revealed anatomical anomalies, including necrosis and cavitation at the junction and accumulation of starch in the rootstock directly below the graft junction. Scion genetics were a significant factor in explaining differences in graft take, and graft take varied from 47% (Criollo 22) to 72% (Pound 7). Rootstock and scion identity both accounted for differences in survival over the course of the 30-month greenhouse study, with a low of 28.5% survival of Criollo 22 scions and a high of 72% for Pound 7 scions. Survival by rootstocks varied from 14.3% on GNV 360 to 100% survival on T 294 rootstock. A positive correlation of 0.34 (p = 0.098) was found between the graft success of different rootstock–scion combinations and their kinship coefficient, suggesting that relatedness of stock and scion could be a driver of incompatibility. Significant rootstock–scion effects were also observed for nutrient use efficiency, plant vigor, and resistance to Phytophthora palmivora. These findings, while preliminary in nature, highlight the potential of rootstock breeding to improve plant nutrition, resilience, and disease resistance in cacao. Full article

This paper presents a novel approach employing localized annealing through Joule heating to enhance the performance of Thin-Film Piezoelectric-on-Silicon (TPoS) MEMS resonators that are crucial for applications in sensing, energy harvesting, frequency filtering, and timing control. Despite recent advancements, piezoelectric MEMS resonators still suffer from anchor-related energy losses and limited quality factors (Qs), posing significant challenges for high-performance applications. This study investigates interface modification to boost the quality factor (Q) and reduce the motional resistance, thus improving the electromechanical coupling coefficient and reducing insertion loss. To balance the trade-off between device miniaturization and performance, this work uniquely applies DC current-induced localized annealing to TPoS MEMS resonators, facilitating metal diffusion at the interface. This process results in the formation of platinum silicide, modifying the resonator’s stiffness and density, consequently enhancing the acoustic velocity and mitigating the side-supporting anchor-related energy dissipations. Experimental results demonstrate a Q-factor enhancement of over 300% (from 916 to 3632) and a reduction in insertion loss by more than 14 dB, underscoring the efficacy of this method for reducing anchor-related dissipations due to the highest annealing temperature at the anchors. The findings not only confirm the feasibility of Joule heating for interface modifications in MEMS resonators but also set a foundation for advancements of this post-fabrication thermal treatment technology. Full article

The efficient thermal management of cutting tools is critical for ensuring dimensional accuracy, surface integrity, and tool longevity, especially in the high-speed dry machining process. However, conventional cooling methods often fall short in reaching the heat-intensive zones near the cutting inserts. This study proposes a novel internal cooling strategy that integrates triply periodic minimal surface (TPMS) structures into the toolholder, aiming to enhance localized heat removal from the cutting region. The thermal-fluidic behaviors of four TPMS topologies (Gyroid, Diamond, I-WP, and Fischer–Koch S) were systematically analyzed under varying coolant velocities using computational fluid dynamics (CFD). Several key performance indicators, including the convective heat transfer coefficient, Nusselt number, friction factor, and thermal resistance, were evaluated. The Diamond and Gyroid structures exhibited the most favorable balance between heat transfer enhancement and pressure loss. The experimental validation confirmed the CFD prediction accuracy. The results establish a new design paradigm for integrating TPMS structures into toolholders, offering a promising solution for efficient, compact, and sustainable cooling in advanced cutting applications. Full article

Creating a comfortable microclimate in the premises of buildings is currently becoming one of the priorities in the field of architecture, construction and engineering systems. The increased attention from the scientific community to this topic is due not only to the desire to ensure healthy and favorable conditions for human life but also to the need for the rational use of energy resources. This area is becoming particularly relevant in the context of global challenges related to climate change, rising energy costs and increased environmental requirements. Practice shows that any technical solutions to ensure comfortable temperature, humidity and air exchange in rooms should be closely linked to the concept of energy efficiency. This allows one not only to reduce operating costs but also to significantly reduce greenhouse gas emissions, thereby contributing to sustainable development and environmental safety. In this connection, this study presents a parametric assessment of the influence of climatic and geometric factors on the aerodynamic characteristics of the air cavity, which affect the heat exchange process in the ventilated layer of curtain wall systems. The assessment was carried out using a combined analytical calculation method that provides averaged thermophysical parameters, such as mean air velocity ($V_s$), average internal surface temperature ($t_{in.s}^{av}$), and convective heat transfer coefficient (${\alpha }_s$) within the air cavity. This study resulted in empirical average values, demonstrating that the air velocity within the cavity significantly depends on atmospheric pressure and façade height difference. For instance, a 10-fold increase in façade height leads to a 4.4-fold increase in air velocity. Furthermore, a three-fold variation in local resistance coefficients results in up to a two-fold change in airflow velocity. The cavity thickness, depending on atmospheric pressure, was also found to affect airflow velocity by up to 25%. Similar patterns were observed under ambient temperatures of +20 °C, +30 °C, and +40 °C. The analysis confirmed that airflow velocity is directly affected by cavity height, while the impact of solar radiation is negligible. However, based on the outcomes of the analytical model, it was concluded that the method does not adequately account for the effects of solar radiation and vertical temperature gradients on airflow within ventilated façades. This highlights the need for further full-scale experimental investigations under hot climate conditions in South Kazakhstan. The findings are expected to be applicable internationally to regions with comparable climatic characteristics. Ultimately, a correct understanding of thermophysical processes in such structures will support the advancement of trends such as Lightweight Design, Functionally Graded Design, and Value Engineering in the development of curtain wall systems, through the optimized selection of façade configurations, accounting for temperature loads under specific climatic and design conditions. Full article

Enhancing damage tolerance and wear resistance in Al–Si-based alloys under thermomechanical stress remains a key challenge in lightweight structural applications. This study investigates the microstructural and tribomechanical behavior of hypoeutectic Al–Si–(Cu)–Mg alloys with varying Cu:Mg ratios (3:1 vs. 1:3) under a T6 heat treatment. Alloys A and B, with identical Si contents but differing Cu and Mg levels, were subjected to multiscale microstructural characterization and mechanical and wear testing at 25 °C, 150 °C, and 250 °C. Alloy A (Cu-rich) exhibited refined α-Al(FeMn)Si phases and homogeneously dissolved Cu in the Al matrix, promoting lattice contraction and dislocation pinning. In contrast, Alloy B (Mg-rich) retained coarse Mg₂Si and residual β-AlFeSi phases, which induced local stress concentrations and thermal instability. Under tribological testing, Alloy A showed slightly higher friction coefficients (0.38–0.43) but up to 26.4% lower wear rates across all temperatures. At 250 °C, Alloy B exhibited a 25.2% increase in the wear rate, accompanied by surface degradation such as delamination and spalling due to β-AlFeSi fragmentation and matrix softening. These results confirm that the Cu:Mg ratio critically influences the dominant hardening mechanism—the solid solution vs. precipitation—and determines the high-temperature performance. Alloy A maintained up to 14.1% higher tensile strength and 22.3% higher hardness, exhibiting greater shear resistance and interfacial stability. This work provides a compositionally guided framework for designing thermally durable Al–Si-based alloys with improved wear resistance under elevated temperature conditions. Full article

P-glycoprotein (P-gp) is a key element of cancer treatment resistance, actively extruding cytotoxic drugs from cells and diminishing their efficacy. While its role at the plasma membrane is well established, its intracellular localization, particularly on lysosomes, is increasingly recognized as a critical contributor to drug resistance. This study investigates four innovative LightSpot fluorescent compounds to detect and quantify both membrane and lysosomal P-gp in Triple-Negative Breast Cancer (TNBC) SUM1315 and DU4475 cell lines. Results highlighted lysosomal P-gp staining by the LightSpot-FL-1, LightSpot-BrX-1, and LightSpot-BdO-1 fluorescent compounds (Mander’s coefficients > 0.8 overlapping with LAMP2 immunostaining). After both cell lines were exposed to Olaparib, a significant increase in P-gp expression level and lysosomal distribution of P-gp was detected. Indeed, after 100 µM Olaparib exposure, LightSpot-FL-1 allowed us to quantify an increase in P-gp-positive lysosome number of 1293 and 334% for SUM1315 and DU4475 cells, respectively, compared to the control. Findings suggest that P-gp may relocate to lysosomes upon drug exposure, highlighting a dual resistance mechanism involving both membrane and lysosomal P-gp. This study demonstrated the potential of LightSpot fluorescent compounds to evaluate P-gp-mediated cell resistance to treatment and emphasized the need to assess global cell P-gp expression to improve cancer diagnosis. Full article

Urbanization has profoundly transformed land surface morphology and amplified thermal environmental modifications, culminating in intensified urban heat island (UHI) phenomena. Local climate zones (LCZs) provide a robust methodological framework for quantifying thermal heterogeneity and dynamics at local scales. Our study investigated the Changsha–Zhuzhou–Xiangtan urban agglomeration (CZXA) as a case study and systematically examined spatiotemporal patterns of LCZs and land surface temperature (LST) from 2002 to 2019, while elucidating mechanisms influencing urban thermal environments and proposing optimized cooling strategies. Key findings demonstrated that through multi-source remote sensing data integration, long-term LCZ classification was achieved with 1,592 training samples, maintaining an overall accuracy exceeding 70%. Landscape pattern analysis revealed that increased fragmentation, configurational complexity, and diversity indices coupled with diminished spatial connectivity significantly elevate LST. Rapid development of the city in the vertical direction also led to an increase in LST. Among seven urban morphological parameters, impervious surface fraction (ISF) and pervious surface fraction (PSF) demonstrated the strongest correlations with LST, showing Pearson coefficients of 0.82 and −0.82, respectively. Pearson coefficients of mean building height (BH), building surface fraction (BSF), and mean street width (SW) also reached 0.50, 0.55, and 0.66. Redundancy analysis (RDA) results revealed that the connectivity and fragmentation degree of LCZ_8 (COHESION8) was the most critical parameter affecting urban thermal environment, explaining 58.5% of LST. Based on these findings and materiality assessment, the regional cooling model of “cooling resistance surface–cooling source–cooling corridor–cooling node” of CZXA was constructed. In the future, particular attention should be paid to the shape and distribution of buildings, especially large, openly arranged buildings with one to three stories, as well as to controlling building height and density. Moreover, tailored protection strategies should be formulated and implemented for cooling sources, corridors, and nodes based on their hierarchical significance within urban thermal regulation systems. These research outcomes offer a robust scientific foundation for evidence-based decision-making in mitigating UHI effects and promoting sustainable urban ecosystem development across urban agglomerations. Full article

In the context of intensified construction and stricter requirements for the energy efficiency of buildings, the use of thermal insulation materials and technologies is becoming particularly important. One promising area in this field is the use of thermal insulation mixtures, which are versatile, adaptable, and highly reliable in operation. Mixtures based on fillers with a porous structure and materials that impart thermal insulation properties, which provide higher thermal insulation properties, are of great interest. However, the development of dry thermal insulation mixtures is hampered by insufficient study of their physical, mechanical, and operational characteristics. This article presents the results of research work on the development and study of dry building thermal insulation mixtures. A distinctive feature of the work is the creation of a composition of dry building thermal insulation mixtures based on local raw materials, such as diatomite, its thermal modification at a temperature of 900 °C, the use of expanded perlite sand, lime, and Portland cement. Research into the properties of modified diatomite has shown that its surface after thermal treatment differs from the surface of unburned diatomite in that it becomes more active and has a 3–4 times higher increase in strength. Modified diatomite and expanded perlite sand have low thermal conductivity, and this property was used in the creation of building thermal insulation mixtures, which was confirmed by research, as the thermal conductivity coefficient ranged from 0.128 to 0.152 W/m °C. The developed dry thermal insulation lime–cement mixture is intended for both interior and exterior finishing works, which is confirmed by the results obtained for determining the frost resistance of the solution and the frost resistance of the contact zone, and corresponds to the F35 grade and has a strength of up to 3.59 MPa. Full article

Harmful gases produced by diesel locomotives tend to accumulate within tunnels, posing risks such as dizziness, vomiting, coma, and even death to the working staff, particularly in long tunnels with high traffic density. As the number of such structures increases, ventilation in extra-long tunnels represents a critical challenge within the engineering area. In this study, the ventilation of an extra-long single-bore double-track tunnel operating with diesel locomotives is investigated. Through scale model tests and based on the inspection sensor data, the natural diffusion patterns of harmful gases under various operating conditions were elucidated. Based on the local resistance coefficient optimization theory and numerical simulations, the ventilation shafts of the tunnel were optimally designed, and an overall ventilation scheme was developed. The ventilation effect of the tunnel was verified through improved scale model tests. The results show that harmful gases primarily diffuse towards the higher elevation tunnel entrance, with only gases near the lower entrance escaping from it. Under the same operating conditions, NO₂ diffuses more slowly than CO, making it harder to discharge. Applying the local resistance coefficient optimization theory, the inclined and vertical shafts of the tunnel can be effectively optimized. The optimized ventilation shafts, coupled with jet fans, can reduce harmful gas concentrations below safety limits within one minute. The methodologies and findings presented here can offer valuable guidance for the ventilation design of similar infrastructures. Full article

It is of the utmost importance to accurately solve the transformer temperature field, as it governs the overall performance and operational stability of the transformer. However, the intricate structure of high- and low-voltage windings, insulating materials, and other components presents numerous challenges for modeling. Temperature exerts a significant influence on insulation aging, and elevated temperatures can notably accelerate the degradation process of insulation materials, reducing their service life and increasing the risk of electrical failures. In view of this, this paper proposes an equivalent modeling method of the temperature field of the transformer HLV winding and studies the refined modeling of the winding part. First of all, in order to reduce the difficulty of temperature field modeling, based on the principle of constant thermal resistance, the fine high- and low-voltage windings are equivalent to large conductors, and the equivalent thermal conductivity coefficient of the high- and low-voltage windings is obtained, which improves the calculation accuracy and shortens the calculation time. Secondly, we verify the feasibility of the equivalent model before and after the simulation, analyze the influence of different boundary conditions on the winding temperature field distribution, and predict the local hotspot location and temperature trend. Finally, a 50 kVA amorphous alloy winding-core transformer is tested on different prototypes to verify the effectiveness of the proposed method. Full article

Background: There are known associations between periodontitis and colorectal cancer, but knowledge on the connections existing between the two are not fully understood. The aim of our study was to assess prevalence and clinical severity of periodontitis in patients with localized colorectal cancer. Secondly, the dynamics of metabolic derangements, particularly glucose metabolism, insulin resistance, and diabetes mellitus were studied as well. Methods: Diagnostic procedures included endoscopies with patohistology, laboratory exams, the insulin resistance homeostatic model assessment method (HOMA index), anthropometrics, and radiology imaging. Periodontal status was evaluated by full-mouth periodontal examination. Results: A total of 79 patients with localized colorectal carcinoma, with slight male predominance of 55.7%, and an age of 65.7 ± 12.4 years participated in this study. Three-quarters of patients (73.4%; 95% CI: 63.0–82.2%) were diagnosed with periodontitis. Patients with periodontitis and colorectal cancer had significantly increased glucose levels (fasting and after oral glucose challenge), (both p < 0.05). Also, increased values of the HOMA index were found in patients with periodontitis vs. controls (without periodontitis) and colorectal cancer; 6.38 ± 5.74 vs. 3.58 ± 2.6 (p = 0.012); Spearman’s Rho correlation coefficient = 0.271 (p = 0.039). There were significant differences in high-density cholesterol in patients with periodontitis vs. controls, 1.41 ± 0.28 vs. 1.23 ± 0.35 mmol/L (p = 0.016), but correlations were insignificant; Rho c.c. = 0.045 (p = 0.738). Conclusions: The most consorted connections between periodontitis and colorectal cancer were found among parameters of metabolic domain, especially glucose concentrations and insulin resistance. Further studies, which would include novel and emerging antidiabetic treatments and their effects on the prevention or control of both diseases, would be warranted. Full article

Estimating the actual evapotranspiration (ET_{c act}) of cropland in arid areas, exploring the time trend, and analyzing periodic variation are the key to long-term assessment of water resource availability and regional drought. The Penman formula has a strong ability to characterize reference crop evapotranspiration (ET_o). However, the application of this formula may be limited in the absence of a complete set of climate data. While previous studies have investigated K_{c act} in China, few have employed localized K_c values to systematically analyze long-term periodic fluctuations in ET_{c act} under climate variability conditions. Therefore, this study aimed to evaluate the applicability of nine ET_o estimation models in the Loess Plateau of China, calculate actual crop coefficients (K_{c act}) for spring maize and winter wheat, and examine the temporal trend and periodicity of ET_{c act} for long-term (1961–2018) continuous cropping of spring maize and winter wheat in the study area. The Mann–Kendall test and continuous wavelet transform (CWT) were used to obtain the temporal trend and periodicity of ET_{c act}. The results were as follows: (1) Priestley–Taylor (Prs–Tylr), based on radiation, and the 1985 Hargreaves–Samani (Harg), based on temperature, can be used when meteorological data are limited. It should be noted that among the models evaluated in this study, except for FAO56-PM, only the Harg equation is compatible with K_c-ET_o due to established conversion factors. (2) The K_{c act} of spring maize at the seeding–jointing stage and the earning–filling stage was 12% and 10% lower than the value recommended by FAO, respectively. For K_{c act} of winter wheat, it was 65% higher, 31% lower, and 85% higher than the FAO experience values in the rejuvenation–jointing stage, heading–grouting stage, and grouting–harvest stage. (3) Winter wheat, through its ET_{c act} cycle synchronized with precipitation and excellent water balance, can effectively alleviate regional drought. It is recommended to be included in the promotion of drought resistance policies. Full article

Gastrointestinal nematode infections are due to a wide number of helminthic genera and species, representing a major concern in goat and sheep farming and leading to different health issues and a general economic loss. Traditional diagnostic tools do not allow for a specific identification and, although a shift towards molecular diagnostic techniques is ongoing, species or genus-specific diagnosis is still poorly implemented. This study describes the development of a novel real-time PCR method for diagnosing Haemonchus sp. and its relative abundance in mixed infections in grazing ruminants. The method employs two primer/probe sets targeting the 18S-rRNA-ITS1-5.8S-ITS2 region: one shared by all strongylids (GEN) and another specific to Haemonchus sp. (HAEM). The method demonstrated optimal efficiency and determination coefficients when applied to serial dilutions of DNA extract. It was then applied in Faecal Egg Count Reduction Test (FECRT) trials conducted on five sheep and five goat farms in northeastern Italy. Seven farms were suspected of overall resistance and only one farm of Heamonchus-related resistance. The results proved the genus-specific approach as valuable in interpreting treatment outcomes and showing concerning levels of anthelmintic treatment ineffectiveness. Further research and sensitization activity is required to encourage the adoption of the method by local farmers and veterinarians. Full article

This study systematically investigates the influence mechanism of the element Cr on the mechanical properties of the heat-affected zone in pipeline steels for supercritical CO₂ transportation. Microstructural evolution in the heat affected-zone was characterized through thermal simulation tests, Charpy impact testing (−10 °C), and microhardness measurements, complemented by multiscale microscopic analyses (optical microscopy, scanning electron microscopy, electron backscatter diffraction, and transmission electron microscopy). The results demonstrate that Cr addition enhances the base metal’s resistance to supercritical CO₂ corrosion but reduces its low-temperature impact toughness from 277 J to 235 J at −10 °C. Notably, the intercritical heat-affected zone exhibits severe embrittlement, with impact energy plummeting from 235 J (base metal) to 77 J. Microstructural analysis reveals that Cr interacts with carbon to form stable carbonitride particles, which reduce the free carbon concentration and diffusion coefficient in austenite, thereby inducing heterogeneous austenitization. Undissolved carbonitrides pin grain boundaries, creating carbon concentration gradients. During rapid cooling, these localized carbon-enriched microregions preferentially transform into core–shell-structured M-A constituent, characterized by a micro-twin containing retained austenite core encapsulated by high hardness lath martensite. The synergistic interaction between micro-twins and interfacial thermal mismatch stress induces localized stress concentration, triggering microcrack nucleation and subsequent toughness degradation. Full article

To validate the long-term performance of self-developed limestone calcined clay cement (LC3), this study evaluated the durability performance of LC3 produced using calcined excavated spoil. Results showed that LC3 exhibited a faster chloride adsorption rate than OPC, achieving peak binding capacity within 14 days, although its total chloride-binding capacity was slightly lower. The chloride diffusion coefficient of LC3 was approximately one order of magnitude lower than that of OPC, enhancing chloride resistance. However, LC3 demonstrated weaker carbonation resistance due to complete decomposition of portlandite (Ca(OH)₂) and ettringite (AFt), alongside partial degradation of calcium silicate hydrate (C-S-H) gels, resulting in pore structure coarsening. Compared to LC3 made with commercial metakaolin (K0), the self-developed LC3 using K1 and K2 clays from excavated spoil showed comparable chloride-binding capacity but slightly weaker chloride penetration resistance. Its carbonation resistance surpassed K0-based LC3. Overall, the self-developed LC3 matched commercial metakaolin-based LC3 in durability, validating the use of locally sourced clays. Producing LC3 from calcined excavated spoil addresses environmental challenges associated with spoil disposal while delivering satisfactory durability. Full article