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22 pages, 11612 KB  
Article
A Novel Method for Reducing Uncertainty in Subglacial Topography: Implications for Greenland Ice Sheet Volume and Stability
by Oliver T. Bartlett and Steven J. Palmer
Remote Sens. 2026, 18(1), 16; https://doi.org/10.3390/rs18010016 (registering DOI) - 20 Dec 2025
Abstract
Subglacial topography is a critical boundary condition for ice sheet models projecting past and future ice sheet–climate interactions. Contemporary ice-sheet-wide bed topography datasets are partially derived using mass conservation, but approximately 75% of the most widely used Greenland Ice Sheet (GrIS) dataset is [...] Read more.
Subglacial topography is a critical boundary condition for ice sheet models projecting past and future ice sheet–climate interactions. Contemporary ice-sheet-wide bed topography datasets are partially derived using mass conservation, but approximately 75% of the most widely used Greenland Ice Sheet (GrIS) dataset is based on simple interpolation of airborne radio-echo sounding (RES) measurements, such as kriging or streamline diffusion. Due to limited independent validation data, the errors and biases in this approach are poorly understood, creating largely unknown uncertainties in subglacial topography. Here, we interpolated synthetic RES observations of bed topography over ice-free areas with a known topography at a 5 m spatial resolution and quantify discrepancies. We found that the absolute error in kriged bed topography increases with distance from the input data, though at a reduced rate than previously estimated. The difference between an interpolated elevation estimate and the local mean elevation is a strong predictor of real bed errors (R2 = 0.72), with further improvement as input observation sparsity increases (R2 > 0.82). We propose a method to quantify and reduce uncertainty in kriged bed topography in sparsely surveyed regions, reducing uncertainty for at least 56% of the kriged interior at a 99% confidence interval. Our results suggest that subglacial depth is on average 5 m deeper than previous estimates, though individual areas may be shallower or deeper (σ = 41 m). Consequently, the area grounded below sea level is likely underestimated by 2%, increasing to 29% for regions deeper than 200 m. These findings have potential implications for the future stability of the GrIS under climate change. Full article
(This article belongs to the Special Issue Remote Sensing of the Cryosphere (Third Edition))
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18 pages, 12047 KB  
Article
Modeling Ice Detachment Events on Cryopumps During Space Propulsion Ground Testing
by Andreas Neumann
Aerospace 2025, 12(12), 1114; https://doi.org/10.3390/aerospace12121114 - 17 Dec 2025
Viewed by 56
Abstract
At DLR’s electric space propulsion vacuum test facility in Goettingen, spontaneous pressure rise events were observed, which led to interruptions of thruster testing. This study investigates the causes of four such events and presents a model that is able to simulate pressure rise [...] Read more.
At DLR’s electric space propulsion vacuum test facility in Goettingen, spontaneous pressure rise events were observed, which led to interruptions of thruster testing. This study investigates the causes of four such events and presents a model that is able to simulate pressure rise events due to xenon ice sheet detachment from operating cryogenic pumps. The model results show good agreement with the observed pressure curves and can reproduce the pressure rise slope, event duration, down slope, and maximum pressure during these events. The masses of the detached xenon ice sheets are in the range from 2 g to 0.4 kg, which is reasonable with respect to the amount of ice on cryopump cold plates. This first modeling step is based on a phenomenological approach, but the good results show that it is worth expanding and refining the model, e.g., by introducing more ice shape options, adding ice bonding layer properties, and adding other gases and physical condensate properties. Full article
(This article belongs to the Section Astronautics & Space Science)
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24 pages, 3342 KB  
Article
Effects of Grass Carp Antifreeze Peptide on Freeze-Thaw Characteristics and Structure of Wet Gluten Protein
by Meizhu Dang, Bing Huang, Yangyang Jia, Yuanyuan Shao, Xingxing Mei and Chunmei Li
Foods 2025, 14(24), 4336; https://doi.org/10.3390/foods14244336 - 16 Dec 2025
Viewed by 155
Abstract
This study uniquely explores the impact of a novel natural antifreeze peptide derived from grass carp (GCAFP) on the freeze–thaw characteristics and structural stability of wet gluten protein, providing new insights into the development of natural cryoprotectants for frozen foods. The effects of [...] Read more.
This study uniquely explores the impact of a novel natural antifreeze peptide derived from grass carp (GCAFP) on the freeze–thaw characteristics and structural stability of wet gluten protein, providing new insights into the development of natural cryoprotectants for frozen foods. The effects of GCAFP on the physicochemical and structural properties of gluten protein were investigated using differential scanning calorimetry (DSC), nuclear magnetic resonance imaging (NMR), rheology, and scanning electron microscopy (SEM). The results showed that the addition of 0.5% GCAFP significantly reduced the freezing temperature (Tf, from −8.50 ± 1.31 °C to −10.75 ± 2.49 °C) and expanded the melting temperature range (Tm,δ, from 3.60 ± 1.40 °C to 5.65 ± 0.12 °C), indicating improved freezing stability. After five weeks of frozen storage, the ice crystal melting enthalpy (ΔHm) of gluten protein in the GCAFP group increased by only 20.17 J/g, compared with 27.23 J/g in the control, representing a 6.35% reduction (p < 0.05). Similarly, after five freeze–thaw cycles, the freezable water fraction (Fw) and ΔHm were reduced by 5.19% and 1.55%, respectively, demonstrating that GCAFP inhibited water migration and ice recrystallization. Low-field NMR revealed that GCAFP maintained a higher proportion of bound water (T21) and decreased free water (T23), confirming its role in restricting water mobility. Rheological analysis showed that GCAFP preserved the viscoelasticity of gluten protein, maintaining higher storage (G′) and loss (G″) moduli than the control after five freeze–thaw cycles, thus mitigating the decline in network elasticity. Structural characterization indicated that GCAFP stabilized the α-helix and β-sheet contents, reduced glutenin macropolymer depolymerization from 24.85% to 18.95%, and strengthened hydrogen bonding within the protein matrix. Overall, GCAFP effectively protected wet gluten protein against ice crystal damage by maintaining water distribution, viscoelasticity, and secondary structure integrity, highlighting its potential as a natural antifreeze ingredient for frozen food applications. Full article
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20 pages, 3497 KB  
Article
Effect of Following Current on the Hydroelastic Behavior of a Floating Ice Sheet near an Impermeable Wall
by Sarat Chandra Mohapatra, Pouria Amouzadrad and C. Guedes Soares
J. Mar. Sci. Eng. 2025, 13(12), 2386; https://doi.org/10.3390/jmse13122386 - 16 Dec 2025
Viewed by 85
Abstract
A theoretical model of the interaction between a following current and a semi-infinite floating ice sheet under compressive stress near a vertical impermeable wall is developed, within the scope of linear water wave theory, to study the hydroelastic behavior. The conceptual framework defining [...] Read more.
A theoretical model of the interaction between a following current and a semi-infinite floating ice sheet under compressive stress near a vertical impermeable wall is developed, within the scope of linear water wave theory, to study the hydroelastic behavior. The conceptual framework defining the buoyant ice structure incorporates the tenets of elastic beam theory. The associated fluid dynamics are governed by strict adherence to the potential flow paradigm. To resolve the undetermined parameters appearing in the Fourier series decomposition of the potential functions, investigators systematically apply higher-order criteria detailing the coupling relationships between modes. The current results are compared with a specific case of results available in the literature, and the convergence analysis of the analytical solution is made for computational accuracy. Further, the free edge conditions are applied at the edge of the floating ice sheet, and the effects of current speed, compressive stress, the thickness of the ice sheet, flexural rigidity, water depth on the strain, displacements, reflection wave amplitude, and the horizontal force on the rigid vertical wall are analyzed in detail. It is found that the higher values of the following current heighten the strain, displacements, reflection amplitude, and force on the wall. The study’s outcomes are considered to benefit not just cold region design applications but also the engineering of resilient floating structures for oceanic and offshore environments, and to the design of marine structures. Full article
(This article belongs to the Section Ocean Engineering)
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17 pages, 12414 KB  
Article
A Spatiotemporal Subgrid Least Squares Approach to DEM Generation of the Greenland Ice Sheet from ICESat-2 Laser Altimetry
by Qiyu Wang, Jinyun Guo, Tao Jiang and Xin Liu
Remote Sens. 2025, 17(24), 4027; https://doi.org/10.3390/rs17244027 - 13 Dec 2025
Viewed by 180
Abstract
Greenland, home to the largest ice sheet in the Northern Hemisphere, provides a crucial digital elevation model (DEM) for understanding polar climate evolution and valuable data for global climate change research. Based on ICESat-2 laser altimetry data collected from satellite observations over Greenland [...] Read more.
Greenland, home to the largest ice sheet in the Northern Hemisphere, provides a crucial digital elevation model (DEM) for understanding polar climate evolution and valuable data for global climate change research. Based on ICESat-2 laser altimetry data collected from satellite observations over Greenland between November 2020 and November 2021, the Shandong University of Science and Technology 2021 DEM (SDUST2021DEM) with 500 m grid resolution at the epoch of May 2021 was constructed using a spatiotemporally fitted subgrid least squares method. The precision of the DEM was evaluated by comparison with National Aeronautics and Space Administration IceBridge data and supplemented by GNSS station measurements. The median difference between the DEM and IceBridge data was −0.33 m, the mean deviation −0.58 m, and the median absolute deviation 2.31 m. The accuracy of SDUST2021DEM exhibits a clear spatial pattern: it is higher in the central ice sheet than at the margins, decreases in regions with complex terrain, and remains more reliable in areas characterized by gentle slopes and flat terrain. Overall, the SDUST2021DEM demonstrates stable accuracy and can reliably produce high-precision DEMs for a specific temporal epoch. Full article
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18 pages, 3591 KB  
Article
Decadal-Scale Warming Signals in Antarctic Ice Sheet Interior Revealed by L-Band Passive Microwave Observations from 2015 to 2025
by Shaoning Lv, Yin Hu and Jun Wen
Remote Sens. 2025, 17(22), 3757; https://doi.org/10.3390/rs17223757 - 19 Nov 2025
Cited by 1 | Viewed by 371
Abstract
The Antarctic ice sheet, Earth’s largest ice mass, is vital to the global climate system. Analyzing its thermal behavior is crucial for sea-level projections and ice shelf assessments; however, internal temperature studies remain challenging due to the harsh environment and limited access to [...] Read more.
The Antarctic ice sheet, Earth’s largest ice mass, is vital to the global climate system. Analyzing its thermal behavior is crucial for sea-level projections and ice shelf assessments; however, internal temperature studies remain challenging due to the harsh environment and limited access to the site. Using ten years of Soil Moisture Active Passive (SMAP) satellite passive microwave brightness temperature (TB) data (2015–2025), we examined changes in TB across Antarctica. Results show a stronger warming trend in West Antarctica, with TB increasing by over 1.5 K over a decade, while East Antarctica remains relatively stable, showing only seasonal summer warming and winter cooling. Furthermore, TB in the Antarctic region correlates best with internal temperatures at depths of 500–2000 m, as indicated by the effective soil temperature, as demonstrated by the modeling data and the τ-z model’s inference. However, the total enthalpy is inconsistent with the TB trend and exhibits the opposite effect when combined with the sensing depth. By comparing the weak trend in surface ice temperature changes, we conclude that the TB warming trend observed on the western side of the Antarctic over the past decade does not originate from the increasing temperatures within the internal ice shelves, which differs from the increase in temperatures at the Antarctic margins. Full article
(This article belongs to the Special Issue Antarctic Remote Sensing Applications (Second Edition))
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17 pages, 2438 KB  
Article
Assessing the Consistency Among Three Mascon Solutions and COST-G-Based Grid Products for Characterizing Antarctic Ice Sheet Mass Change
by Qing Long and Xiaoli Su
Remote Sens. 2025, 17(22), 3699; https://doi.org/10.3390/rs17223699 - 12 Nov 2025
Viewed by 389
Abstract
To facilitate easy accessibility to the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On (GRACE-FO) results for the geoscientific community, multiple institutions have successively developed mass anomaly grid products including mass concentration (mascon) grids; these were provided at the Gravity Information Service [...] Read more.
To facilitate easy accessibility to the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On (GRACE-FO) results for the geoscientific community, multiple institutions have successively developed mass anomaly grid products including mass concentration (mascon) grids; these were provided at the Gravity Information Service (GravIS) portal. However, an assessment of their consistency for studying large-scale mass redistribution and transport in Earth’s system is still not available. Here, we compare three major mascon solutions separately from the Center for Space Research (CSR), the Jet Propulsion Laboratory (JPL), the Goddard Space Flight Center (GSFC) and GravIS products based on the Combination Service for Time-variable Gravity fields (COST-G) by analyzing the Antarctic Ice Sheet (AIS) mass changes in four aspects. Our results demonstrate that: (1) the four datasets exhibit strong consistency on the entire AIS mass change time series, with the largest difference occurring in the Antarctic Peninsula; (2) mass trend estimates show better agreement over longer periods and larger regions, but differences with a percentage of 20–40 exist during the late stage of GRACE and the whole GRACE-FO timespan; (3) notable discrepancies arise in the annual statistics of the Eastern AIS in 2016, leading to inconsistency on the sign of annual AIS mass change; (4) good agreement can be seen among these interannual mass variations over the AIS and its three subregions during 2003–2023, excluding the period from mid-2016 to mid-2018. These findings may provide key insights into improving algorithms for mascon solutions and grid products towards refining their applications in ice mass balance studies. Full article
(This article belongs to the Special Issue Earth Observation of Glacier and Snow Cover Mapping in Cold Regions)
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14 pages, 2510 KB  
Article
Experimental Study on the Mechanical Response and Failure Behavior of High-Pressure Frozen Ice Under Axial Loading Conditions
by Zhijiang Yang, Yu Zhang, Tao Han, Ying Ding, Chenyi Zhang and Weihao Yang
Geosciences 2025, 15(11), 422; https://doi.org/10.3390/geosciences15110422 - 5 Nov 2025
Viewed by 364
Abstract
The mechanical response and failure behavior of high-pressure frozen ice are essential to the technological progress in drilling thick polar ice sheets, but current research primarily focuses on non-pressure-frozen ice. In this paper, ice specimens with a cylindrical geometry were fabricated at −20 [...] Read more.
The mechanical response and failure behavior of high-pressure frozen ice are essential to the technological progress in drilling thick polar ice sheets, but current research primarily focuses on non-pressure-frozen ice. In this paper, ice specimens with a cylindrical geometry were fabricated at −20 °C, applying freezing pressures across a range of 10 to 40 MPa with a 10 MPa interval. Their mechanical properties were investigated through triaxial compression tests under axial loading conditions and were compared with the results obtained at −10 °C. The results indicate that, with increasing freezing pressure, the samples transitioned from a failure state of interlaced cracking to a highly transparent state. The failure behavior observed in the specimens was characterized as ductile, as evidenced by the deviatoric stress–axial strain relationships. Moreover, the peak deviatoric stress exhibited a non-monotonic dependence on freezing pressure, with an initial rise from 9.59 MPa at 10 MPa to a peak of 14.37 MPa at 30 MPa and a subsequent decline to 10.12 MPa at 40 MPa. All specimens reached a relatively stable residual state at 5% axial strain, with residual deviatoric stresses ranging from 4.13 to 5.71 MPa. A reduction in freezing temperature from −10 °C to −20 °C can effectively enhance both the peak deviatoric stress and the residual stress of high-pressure frozen ice under triaxial shear conditions. All peak tangent modulus values, ranging from 1.61 to 2.93 GPa with an average of 2.2 GPa, were observed within 0.7% axial strain and exhibited mild fluctuations with increasing freezing pressure. These findings provide a more robust mechanical foundation for drilling research and operations in extremely thick polar ice caps. Full article
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30 pages, 2309 KB  
Article
Annual and Interannual Oscillations of Greenland’s Ice Sheet Mass Variations from GRACE/GRACE-FO, Linked with Climatic Indices and Meteorological Parameters
by Florent Cambier, José Darrozes, Muriel Llubes, Lucia Seoane and Guillaume Ramillien
Remote Sens. 2025, 17(21), 3552; https://doi.org/10.3390/rs17213552 - 27 Oct 2025
Viewed by 755
Abstract
The ongoing global warming threatens the Greenland Ice Sheet (GIS), which has exhibited an overall mass loss since 1990. This loss varies annually and interannually, reflecting the intricate interactions between the ice sheet and atmospheric and oceanic circulations. We investigate GIS mass balance [...] Read more.
The ongoing global warming threatens the Greenland Ice Sheet (GIS), which has exhibited an overall mass loss since 1990. This loss varies annually and interannually, reflecting the intricate interactions between the ice sheet and atmospheric and oceanic circulations. We investigate GIS mass balance variations (2002–2024) using data from the Gravity Recovery and Climate Experiment (GRACE) and its Follow-On (GRACE-FO) missions. Monthly mass anomalies from the International Combination Service for Time-variable Gravity Fields (COST-G) solution are compared with cumulative climate indices (North Atlantic Oscillation—NAO, Greenland Blocking Index—GBI, Atlantic Multidecadal Oscillation—AMO) and meteorological parameters (temperature, precipitation, surface albedo). Empirical Orthogonal Function analysis reveals five principal modes of variations, the first capturing annual and interannual frequencies (4–7 and 11 years), while subsequent modes only describe interannual frequencies. Wavelet analysis shows significant annual correlations between GIS mass changes and temperature (r = −0.88), NAO (r = 0.74), and GBI (r = −0.85). An annual cycle connects GIS mass changes, climatic indices, and meteorological parameters, while interannual variations highlight the role of the AMO and the NAO. The presence of an 11-year periodicity with the mass variations for NAO, GBI, and temperature strongly correlates with solar activity. Full article
(This article belongs to the Special Issue Space-Geodetic Techniques (Third Edition))
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14 pages, 1942 KB  
Article
The Late Glacial Advance of the James Lobe, South Dakota, Suggests Climate-Driven Laurentide Ice Sheet Behavior
by Stephanie L. Heath and Thomas V. Lowell
Quaternary 2025, 8(4), 58; https://doi.org/10.3390/quat8040058 - 22 Oct 2025
Viewed by 779
Abstract
The relationship between climate and independent glacier masses is now understood, but what is not understood is how ice sheets respond during times of rapid climate change. At its maximum extent the southern Laurentide Ice Sheet (LIS) was sourced from two domes that [...] Read more.
The relationship between climate and independent glacier masses is now understood, but what is not understood is how ice sheets respond during times of rapid climate change. At its maximum extent the southern Laurentide Ice Sheet (LIS) was sourced from two domes that terminated in multiple lobes across central North America. The extent and timing of the eastern lobes, which were sourced from the Labrador Dome are relatively well constrained. Although the extent of the lobes sourced from the western Keewatin Dome is better understood, there is little chronologic data on them. Twenty-six radiocarbon ages recovered from within the drift of the James Lobe from South Dakota are used to reconstruct the timing of late-glacial fluctuations of the James Lobe. Lithologic logs from 21 South Dakota counties were analyzed and provide stratigraphic context for the radiocarbon ages. Analysis of the stratigraphy reveals two distinct glacial till units with a distinct, widespread layer of silt between them. The silt is interpreted here as evidence for interstadial conditions between two separate advances of the James Lobe. Radiocarbon ages of organics from this silt layer and from within the uppermost oxidized till indicate that interstadial conditions persisted from ~15.8 to 13.7 ka, followed by an advance of the James Lobe of at least 230 km to its maximum position at the Missouri River. Comparison to other locations in Wisconsin, northern lower Michigan, and western New York reveals a similar period of interstadial conditions followed by ice margin advance. We correlate this advance across ~1000 km and suggest that the simplest explanation is reduced summer ablation caused by widespread climatic cooling. Full article
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23 pages, 3611 KB  
Article
Microstructural Evolution of Antarctic Ice with the Rising Atmospheric CO2: A Longitudinal Meta-Analysis
by Vuk Uskoković
Quaternary 2025, 8(4), 57; https://doi.org/10.3390/quat8040057 - 21 Oct 2025
Viewed by 738
Abstract
Antarctica, largely free from geopolitical borders, serves as a critical site for scientific research, environmental monitoring and climate studies. The continent’s ice cap holds over 60% of the Earth’s freshwater and provides a stable climatological record spanning 800,000 years. In this study, the [...] Read more.
Antarctica, largely free from geopolitical borders, serves as a critical site for scientific research, environmental monitoring and climate studies. The continent’s ice cap holds over 60% of the Earth’s freshwater and provides a stable climatological record spanning 800,000 years. In this study, the relationship between changes in atmospheric CO2 levels over the past century and the microstructural characteristics of Antarctic ice was investigated. While it is well-documented that CO2 fluctuations have driven the periodic expansion and retreat of ice sheets, no research to this day has explored how variations in CO2 concentrations influence the physical integrity of ice at the microscopic scale. To address this, grain size, anisotropy, irregularity, and solidity of surface and near-surface ice samples collected over the past 70 years were analyzed. These microstructural features were compared against historical atmospheric greenhouse gas data from multiple Antarctic research stations, including records from the Scripps Institution of Oceanography, the Japanese Antarctic Research Expedition, and the NOAA Global Monitoring Laboratory. Results reveal a correlation between rising CO2 levels and changes in ice microstructure, particularly an increase in the grain size as well as the reduction in the grain aspect ratio and in the morphological solidity. The study remains limited by significant sources of variability, including differences in sampling depths, geographical locations, seasonal effects, and inconsistencies in analytical tools and methodologies reported across the literature. Despite these limitations, this proof-of-concept study elicits the need for continued meta-analyses of existing climate datasets. Such efforts could provide deeper insights into the role of greenhouse gas concentrations in defining the microstructural stability of Antarctic ice, which is critical for predicting ice sheet integrity and its contribution to sea level rise. Full article
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14 pages, 4613 KB  
Article
Exploring Trends in Earth’s Precipitation Using Satellite-Gauge Estimates from NASA’s GPM-IMERG
by José J. Hernández Ayala and Maxwell Palance
Earth 2025, 6(4), 130; https://doi.org/10.3390/earth6040130 - 17 Oct 2025
Viewed by 1560
Abstract
Understanding global precipitation trends is critical for managing water resources, anticipating extreme events, and assessing the impacts of climate change. This study analyzes spatial and temporal patterns of precipitation from 1998 to 2024 using NASA’s Global Precipitation Measurement Mission (GPM) Integrated Multi-satellite Retrievals [...] Read more.
Understanding global precipitation trends is critical for managing water resources, anticipating extreme events, and assessing the impacts of climate change. This study analyzes spatial and temporal patterns of precipitation from 1998 to 2024 using NASA’s Global Precipitation Measurement Mission (GPM) Integrated Multi-satellite Retrievals for (IMERG) Version 7, which merges satellite observations with rain-gauge data at 0.1° resolution. A total of 324 monthly datasets were aggregated into annual and seasonal composites to evaluate annual and seasonal trends in global precipitation. The non-parametric Mann–Kendall test was applied at the pixel scale to detect statistically significant monotonic trends, and Sen’s slope estimator method was used to quantify the magnitude of change in mean annual and seasonal global precipitation. Results reveal robust and geographically consistent patterns: significant wetting trends are evident in high-latitude regions, with the Arctic and Southern Oceans showing the strongest increases across multiple seasons, including +0.04 mm/day in December–January–February for the Arctic Ocean and +0.04 mm/day in June–July–August for the Southern Ocean. Northern China also demonstrates persistent increases, aligned with recent intensification of extreme late-season precipitation. In contrast, significant drying trends are detected in the tropical East Pacific (up to −0.02 mm/day), northern South America, and some areas in central-southern Africa, highlighting regions at risk of sustained hydroclimatic stress. The North Atlantic south of Greenland emerges as a summer drying hotspot, consistent with Greenland Ice Sheet melt enhancing stratification and reducing precipitation. Collectively, the findings underscore a dual pattern of wetting at high latitudes and drying in tropical belts, emphasizing the role of polar amplification, ocean–atmosphere interactions, and climate variability in shaping Earth’s precipitation dynamics. Full article
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24 pages, 5190 KB  
Article
Study on Stage Characteristics and Multi-Factor Optimization Regulation of Performance of Ice Thawing Agent in Low Temperature Environment
by Junming Mo, Ke Wu, Lei Qu, Wenbin Wei and Jinfu Zhu
Appl. Sci. 2025, 15(20), 10865; https://doi.org/10.3390/app152010865 - 10 Oct 2025
Viewed by 447
Abstract
De-icing agents play a crucial role in winter road maintenance, yet their excessive application can result in pavement deterioration and environmental issues. Existing dosage guidelines lack comprehensive data on the dynamic response of de-icing agents under low-temperature conditions, particularly regarding stage-specific characteristics and [...] Read more.
De-icing agents play a crucial role in winter road maintenance, yet their excessive application can result in pavement deterioration and environmental issues. Existing dosage guidelines lack comprehensive data on the dynamic response of de-icing agents under low-temperature conditions, particularly regarding stage-specific characteristics and multi-factor interactions. This research systematically evaluated the effectiveness of four de-icing agents (NaCl, CaCl2, MgCl2, CH3COOK) within a temperature range of −5 °C to −25 °C, elucidating the two-phase ice-melting process (solid-phase followed by salt solution de-icing) with distinct kinetic mechanisms—a previously underexplored temporal pattern. The study quantified the differential impacts of particle size (small-particle CaCl2 exhibiting 12% higher efficiency than sheet-like forms), dosage linear correlation, and negligible effects of ice layer thickness and road surface composition, which have not been systematically validated in prior studies. Temperature sensitivity was further refined: NaCl showed a 42.4% efficiency drop between −5 °C and −25 °C, while MgCl2 maintained stable performance, supporting its potential as an environmentally sustainable alternative. This work provides a quantitative basis for dynamic dosage regulation by integrating stage characteristics and multi-factor optimization, addressing gaps in existing guidelines. Full article
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15 pages, 6815 KB  
Article
Structural Characterization, Cytotoxicity and Microbiological Activity of One-Step-Synthesized RGO/AuNPs Nanocomposites
by Boris Martinov, Dimitar Dimitrov, Tsvetelina Foteva, Aneliya Kostadinova and Anna Staneva
Materials 2025, 18(19), 4464; https://doi.org/10.3390/ma18194464 - 25 Sep 2025
Viewed by 514
Abstract
This study presents a green, single-step method for synthesizing nanocomposites based on reduced graphene oxide (RGO) and gold nanoparticles (AuNPs), using sodium citrate as a mild reducing and stabilizing agent. AuNPs were generated from chloroauric acid (HAuCl4) directly on the surface [...] Read more.
This study presents a green, single-step method for synthesizing nanocomposites based on reduced graphene oxide (RGO) and gold nanoparticles (AuNPs), using sodium citrate as a mild reducing and stabilizing agent. AuNPs were generated from chloroauric acid (HAuCl4) directly on the surface of graphene oxide (GO), which was simultaneously reduced to RGO. Structural characterization via Transmission Electron Microscopy (TEM), High Resolution TEM (HRTEM) and Selected Area Electron Diffraction (SAED) confirms spherical AuNPs (10–60 nm) distributed on RGO sheets, with indications of nanoparticle aggregation. Dynamic Light Scattering (DLS) and zeta potential analysis support these findings, suggesting colloidal instability with higher RGO content. Biological evaluation demonstrates dose-dependent cytotoxicity in HaCaT keratinocytes, with IC50 values (half maximal inhibitory concentration) decreasing as RGO content is increased. At moderate dilutions (1–25 µL/100 µL), the composites show acceptable cell viability (>70%). Antibacterial assays reveal strong synergistic effects against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, with sample RGO/Au 0.500/0.175 g/L showing complete E. coli inhibition at low Au content (0.175 g/L). The composite retained activity even in protein-rich media, suggesting potential for antimicrobial applications. These findings highlight the potential of RGO/AuNPs composites as multifunctional materials for biomedical uses, particularly in antimicrobial coatings and targeted therapeutic strategies. Full article
(This article belongs to the Section Electronic Materials)
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21 pages, 13388 KB  
Article
Genome-Wide Identification of the CBL-CIPK Gene Family in the Ice Plant and Functional Analysis of Salt Stress Tolerance
by Can Wang, Nan Li, Haifeng Sun, Tianyue Xu, Jinghua He, Chenhao Zhang, Zipeng Meng, Xinyao Zhang, Rong Zhou, Yingchao Zhang and Xiaoming Song
Life 2025, 15(9), 1476; https://doi.org/10.3390/life15091476 - 19 Sep 2025
Cited by 1 | Viewed by 549
Abstract
Background: The ice plant (Mesembryanthemum crystallinum L.) is a typical halophyte with remarkable stress resistance traits, including salinity and alkalinity tolerance. As a crucial signaling transduction pathway for plant responses to environmental stress, the CBL-CIPK signaling system plays a key role [...] Read more.
Background: The ice plant (Mesembryanthemum crystallinum L.) is a typical halophyte with remarkable stress resistance traits, including salinity and alkalinity tolerance. As a crucial signaling transduction pathway for plant responses to environmental stress, the CBL-CIPK signaling system plays a key role in regulating plant stress resistance. Methods: This study systematically analyzed the composition characteristics of the CBL and CIPK gene families across 24 plant species, including the ice plant, using comparative genomics approaches. Results: A total of 297 CBL and 561 CIPK gene family members were identified across the 24 species. Within the ice plant genome, 9 CBL and 18 CIPK genes were identified. Compared to model plants like Arabidopsis thaliana, the ice plant possesses a relatively higher number of CIPK genes, which may be related to its specific adaptation to saline–alkaline environments. Phylogenetic analysis indicated that the ice plant CBL and CIPK genes could be classified into three and four subfamilies, respectively. Expression analysis revealed that several genes (e.g., McCBL1, McCBL4, McCIPK1, McCIPK2) were significantly upregulated under salt stress, suggesting their important roles in the salt stress response. Notably, ice plant CBL and CIPK genes exhibit significant structural diversity. For instance, McCBL3 contains significantly more CDS regions than other members, while CIPK genes can be divided into two types: single-CDS type and multi-CDS type. This structural variation may be associated with functional divergence during the evolution of the gene family. Furthermore, three-dimensional (3D) structure prediction showed that CBL proteins primarily consist of EF-hand domains and α-helices, whereas CIPK proteins additionally contain β-sheet domains, implying that this structural difference may be related to their distinct regulatory mechanisms. Conclusions: This study provides an important theoretical basis for a deeper understanding of the molecular mechanisms underlying the CBL-CIPK signaling pathway in the saline–alkaline stress response of the ice plant. Full article
(This article belongs to the Section Plant Science)
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