Search Results (2,490)

Bottle-brush polymers with aggrecan-like side chains represent a class of biomimetic macromolecules that replicate key structural and functional features of natural complexes of aggrecans with hyaluronic acid (HA) which are the major components of articular cartilage. In this study, we employ numerical self-consistent field (SCF) modeling combined with analytical theory to investigate the conformational properties of cylindrical molecular bottle-brushes composed of aggrecan-like double-comb side chains tethered to the main chain (the backbone of the bottle-brush). We demonstrate that the architecture of the brush-forming double-comb chains and, in particular, the distribution of polymer mass between the root and peripheral domains significantly influences the spatial distribution of primary side chain ends, leading to formation of a “dead” zone near the backbone of the bottle-brush and non-uniform density profiles. The axial stretching force imposed by grafted double-combs in the main chain, as well as normal force acting at the junction point between the bottle-brush backbone and the double-comb side chain are shown to depend strongly on the side-chain architecture. Furthermore, we analyze the induced bending rigidity and persistence length of the bottle-brush, revealing that while the overall scaling behavior follows established power laws, the internal structure can be finely tuned without altering the backbone stiffness. These theoretical findings provide valuable insights into relations between architecture and properties of bottle-brush-like supra-biomolecular structures, such as aggrecan-hyaluronan complexes. Full article

The COVID-19 pandemic forced European Union member states to accelerate the digitalization of public services, turning a gradual policy priority into an urgent necessity. This study examines the pandemic’s impact on the digital transformation of public administrations, assessing the effectiveness of digital-oriented interventions implemented during this period. Using a Difference-in-Differences (DiDs) methodology, the analysis compares treatment and control groups based on 2019 Digital Economy and Society Index (DESI) scores, with digital public services as the dependent variable. Independent variables include pre-filled forms, service transparency, design and data protection, e-government usage, internet penetration, total population, and governance quality, covering all 27 EU member states from 2016 to 2023. Data sources include DESI, Eurostat, and the World Bank. The analysis shows that countries with lower digitalization achieved the largest post-pandemic gains, with transparency, service design, and data protection significantly enhancing digital service quality. Pre-existing governance and infrastructure shaped the magnitude of these improvements, highlighting the combined role of preparedness and reactive policy measures. The findings underscore the critical role of citizens as end-users and accountability drivers in digital governance. By providing empirical evidence on pandemic-driven digitalization trends, this study contributes to policy discussions on resilience, strategic planning, and the future of inclusive, transparent e-government services in the EU. Full article

Myofibroblasts drive wound healing and fibrotic disease through generation of contractile force to promote wound closure and production of matrix proteins to generate scar tissue. Platelets secrete many pro-wound healing molecules, including cytokines and growth factors. We previously reported that inorganic polyphosphate, secreted by activated platelets, is chemotactic for fibroblasts and induces a myofibroblast phenotype. Using NIH-3T3 cells and primary human fibroblasts, we examined the impact of inhibitors of cell-surface receptors and intracellular signaling molecules on polyphosphate-induced myofibroblast differentiation. We now report that polyphosphate-induced differentiation of fibroblasts to myofibroblasts occurs through a signaling pathway mediated by the receptor for advanced glycation end products (RAGE) and nuclear factor kappa B (NFκB) transcription factor. Inhibition of these signaling components ablated the effects of polyphosphate on fibroblasts. Platelet releasates also induced NFκB signaling and myofibroblast differentiation. Blocking the polyphosphate content of platelet releasates with a biocompatible polyP inhibitor rendered the releasates unable to induce myofibroblast differentiation. These results identify a cell-surface receptor and intracellular transcription factor utilized by platelet polyphosphate to promote wound healing through myofibroblast differentiation and may provide targets for promoting wound healing or altering the disease progression of fibrosis. Full article

Due to the random factors that influence grinding stability, hardness distribution appears in inhomogeneity at different locations on the hardened layer in grind-hardening technology. It may affect the wear resistance of parts. Therefore, in order to explore the influence mechanism of hardness homogeneity on the wear resistance comprehensively, grind-hardening and friction experiments on AISI 1045 steel are carried out. Then, the causes of inhomogeneous hardness distribution are analyzed, and the influence of hardness homogeneity on wear resistance is also discussed. Combining the Archard wear model, the wear process of the hardened layer is simulated for analyzing the effect of contact stress distribution and action range on material loss in the worn area and finally realizing the prediction of the wear depth. The results show that the difference in microstructure distribution caused by the nonlinear variation in grinding force is the fundamental reason for the hardness inhomogeneity of the hardened layer. The hardness homogeneity results in the wear resistance of the cut-out end being superior to that of cut-in end. Additionally, the error between the predictive and the experimental value of the wear depth with different parameters is between 3.6% and 11.3%, thereby verifying the effectiveness of the theoretical research. Full article

Microbial safety in low-moisture foods (LMFs) has attracted widespread public attention due to the multiple outbreaks and recalls in recent years. Dry cleaning methods are typically used in LMFs production environments. However, there is no standardized and consistent method for controlling normal force and measuring the shear force of cleaning tool applied on food contact surfaces during dry cleaning. A dry-cleaning platform with the normal force controllable feature was custom-designed, and its performance was evaluated as the primary objective of the study. Effects of various factors (bacterial type, surface material, surface roughness, and normal force) on the shear force and removal of Salmonella enterica Enteritidis PT 30 (S. PT 30) and Enterococcus faecium NRRL B2354 (E. faecium) during dry wiping were investigated using the developed platform. The performance evaluation indicated that the platform was adequately stable during standardized and consistent dry cleaning. Surface roughness, normal force, and surface material significantly affected shear force (p < 0.05) applied on food contact surfaces. The bacterial type significantly affected the shear force on stainless steel (p < 0.05). No significant difference (p > 0.05) was observed in removing S. PT 30 from inoculated surfaces after dry wiping under all investigated conditions. Surface material significantly affected the removal of E. faecium (p < 0.05). The removal of E. faecium was numerically higher than that of Salmonella under the same conditions. Thus, E. faecium may not be a suitable surrogate for S. PT 30 removal at the end of dry cleaning under the wiping conditions tested. The potential applications of the platform were also discussed for future studies on how to enhance dry cleaning efficiency. Shear force can guide the disruption of cohesion and adhesion in surface microorganisms/residues, thereby enhancing cleaning efficiency. The custom-designed dry-cleaning platform with the controllable normal force feature has potential applications in further laboratory dry cleaning studies. Full article

This work presents the development of carbon nanotubes functionalised with carboxylic acid groups (CNT-COOH) as an oxygen-sensitive electrochemical platform for parallel multi-analyte enzymatic biosensing. The platform was constructed by depositing carboxylic-acid-functionalised single-walled carbon nanotubes covalently onto nanostructured gold electrodes modified with a self-assembled monolayer of 4-aminothiophenol. Atomic force microscopy characterization revealed that the nanotubes attached via their ends to the surface and had a predominantly horizontal orientation. Glucose oxidase, lactate oxidase, glutamate oxidase, and tyrosinase were immobilised onto the electrodes to create selective biosensor for lactate, glucose, glutamate, and dopamine, respectively. A key finding is that incorporating catalase significantly extends the linear detection range for analytes by mitigating the accumulation of hydrogen peroxide. The resulting multifunctional biosensor demonstrated its capability for the simultaneous and independent measurement of glucose, lactate as the key bioanalytes under uniform conditions in blood plasma samples, highlighting its potential for applications in health and food technologies. Full article

The mechanical properties of screw-thread steel bars used for prestressing concrete and their threaded ribs’ bearing mechanism have not been quantitatively studied, in contrast to the extensive qualitative research on ordinary steel mechanical connection splices. A quantitative investigation was conducted under various design parameters and working conditions to examine the mechanical connection splices of screw-thread steel bars used for prestressing concrete. The splices’ connection performance and their threaded ribs’ bearing mechanism were also examined. Analyzing the force on the threads of the splices under monotonic tensile loading allowed for the theoretical computation of the axial force coefficients for threaded ribs. The validated revised three-dimensional numerical model of splices is based on the findings of the theoretical calculations. Afterwards, rigorous numerical simulations of monotonic tensile loading, repeated tensile and compressive loading with high stress, and repeated tensile and compressive loading with large strain were performed on 45 splices with varying nominal rebar diameters, coupler outer diameters and lengths, and thread rib spacings. The results show that rebar pullout and rebar fracture are the two main ways in which splices might fail. After cyclic loading, the splices’ ultimate bearing capacity changed by 0.83% to 2.81%, and their ductility changed by 2.13% to 4.75% compared to after monotonic tensile loading. Although the splice load-carrying capacity and plastic deformation capacity were reduced by 2.11%~7.48% and 3.98%~25.78%, respectively, when the thread rib spacing was increased from the specified value to 0.6~0.8 times the nominal diameter of the rebar, the splice connection performance was still able to meet the requirements for class I splices. Approximately half of the splices’ load-bearing capability is provided by the 1–2 turns of threads close to the coupler ends; after cyclic loading, their stress rises by between 4.52% and 12.63% relative to monotonic tension. Stresses in all threaded ribs of the splices are increased by 5.49% to 27.76% as the distance between the threaded ribs increases to 1.0 and 1.2 times the nominal diameter of the rebar, which reduces the splice’s load-bearing capacity. Full article

In this paper, we proposed a novel method for grinding trajectory planning on large-curved forgings to improve grinding performance and grinding efficiency. Our method consists of four main steps. Firstly, we conducted simulations and analyses on the contact state and contact pressure between the grinding tool and curved workpieces, and explored different grinding methods. Based on the Preston equation, a material removal model was established to analyze the grinding force. Secondly, we proposed an adaptive impedance control method based on grinding force analysis, which can control the contact force indirectly by adjusting the end position of the robot. To address the inability of impedance control to adjust impedance parameters in real time, a control strategy involving online estimation of environmental position and stiffness is adopted. Based on the Lyapunov asymptotic stability principle, an adaptive impedance control model is established, and the effectiveness of the adaptive algorithm is verified through simulation. Thirdly, Position correction is realized through gravity compensation of the grinding force and discretization of the impedance control model. Subsequently, a dynamic trajectory adjustment strategy is proposed, which integrates position correction for the current grinding point and position compensation for the next grinding point, to achieve the force control objective in the grinding process. Finally, a constant force grinding experiment was conducted on large-curvature blades using a robotic automatic grinding system. The grinding system effectively removed the knife marks on the blade surface, resulting in a surface roughness of 0.5146 μm and a grinding efficiency of approximately 0.89 cm²/s. The simulation and experimental results indicate that the smoothness and grinding efficiency of the blades are superior to the enterprise’s existing grinding technology, verifying the feasibility and effectiveness of our proposed method. Full article

For military missions, the helicopter is a versatile aircraft with many benefits. However, the high levels of noise radiation enable large acoustic detection ranges. For mission success, knowledge of acoustic radiation and propagation is paramount. This paper describes the results of acoustic measurements for a helicopter that is typically used for operations by the German special forces. The measurements include identification of the global noise radiation during different flight conditions, determination of the time between acoustic detection and arrival at the target location, and determination of the acoustic detection distance. Different approaches and departure procedures were executed, and the acoustic radiation was measured. Results indicate that during approaches, the most noise is generated either during the transition from steady level flight to descent/deceleration or at the end of the flare procedure. For the helicopter considered here, a left turn departure generates more noise at a target location then a right turn departure. Full article

The genetic material in living systems is mainly stored in DNA molecules, which in turn play a dominant biological role in relation to the coding and transfer of genetic information, the biosynthesis of proteins and RNA and the packaging and regulation of DNA expression and accessibility. These features, strictly dictated by the three-dimensional structure of DNA, are governed by non-covalent chemical interactions that drive the folding process of these biological macromolecules. The Main Mechanical Forces (MMFs) approach is a recently formulated calculation method, based on the accurate prediction of structural features of biomolecules through an in-depth assessment of the interplay between specific non-covalent chemical interactions and related mechanical forces developed during the folding process. By adopting the MMFs method in the context of nucleic acids, we report here the results obtained in terms of predicting three-dimensional DNA oligomer tertiary structures. To this end, we have developed tailored nucleic acid-specific equations, enabling to predict the torsion angles (with a relevant level of agreement with experimental values) of the phosphate-sugar backbone of the three model molecules A-, B- and Z- DNA used in this study. To increase the validity of this methodology, we have conducted RMSD measurements, indicating that there is a weak but rather acceptable match between the calculated vs. predicted A-DNA structure, whereas the prediction of the BII-DNA and Z-DNA tertiary structures was fully correct. Full article

This study systematically investigated the structural damage and electrochemical performance changes in 18650 cylindrical lithium-ion batteries under multiple impacts through a 10 kg drop-hammer impact test. The experimental results showed that as the state of charge (SOC) increased from 25% to 75%, the battery’s stiffness increased and its impact resistance improved, but the electrolyte leakage intensified, with a higher risk of leakage at high SOCs. An increase in the impact force led to enhanced voltage fluctuations and a continuous increase in deformation. After an impact of 500 mm, the voltage decreased about 0.02 V, while after an impact of 1000 mm, it dropped about 0.04 V. Axial impacts caused a sudden voltage drop to 1.96 V, resulting in permanent failure; compared with planar impacts, cylindrical surface impacts are more likely to cause compression in the middle and warping at both ends, significantly increasing the risk of internal short circuits. CT scans revealed that the battery porosity can reach up to 3.09% under high impact energy, and the deformation rate can reach 28.39%. The research results provide a quantitative experimental basis for the impact-resistant design and safety assessment of power batteries. Full article

This article presents an original experimental and numerical approach to examining the pull-out behavior of fastening systems made of steel bars simultaneously embedded in both ends of concrete samples. This double-embedded configuration simulates a connection between the existing concrete structure and a new external exoskeleton, promoting seismic strengthening. Pull-out tests were performed across six specimen configurations combining different concrete strength classes in order to compare the adhesive solution against traditional monolithic cast-in rebar embedments. The adhesive-anchored bars achieved a peak pull-out force of ~28.6 kN, which is about 18% higher than with mixed anchorage (one end adhesive, one end cast-in). All specimens failed in concrete cracking and pull-out cone formation, with no steel bar yielding, indicating that failure was governed by concrete strength. Finite element simulations in ANSYS Explicit Dynamics were validated against these experiments, confirming the observed behavior and enabling the extension of our analysis to broader concrete strength ranges. Overall, the results demonstrate that double-ended adhesive anchorage significantly increases the connection’s load-bearing capacity and ductility compared to mixed configurations. Full article

Shaft deflection degrades roller alignment and intensifies stress concentration/edge effects at roller-ends and raceway edges, ultimately compromising service performance of tapered roller bearings (TRBs). Therefore, a dynamic model was developed for a TRB subjected to a deflected shaft in which Johnson’s load–deformation relationship was applied to reflect non-uniform cross-sectional structures of the tapered rollers and raceways, viscous damping was integrated into the roller/cage interaction, and friction actions at the raceways and flange areas were treated separately. Then, moment load and angular misalignment of the tapered roller were analyzed under various shaft deflection and operating conditions. Results indicate that tilt angle remains orders of magnitude smaller than skew angle. Shaft deflection amplifies both skew and tilt, and the influence level is proportional to the bearing size. Centrifugal effect primarily affects skew motion, whereas gyroscopic effect mainly influences tilt motion. Axial forces exert greater influence on roller skew than tilt. The flange typically constrains roller skew, whereas both raceways may induce bidirectional tilt/skew motion. Full article

Left ventricular (LV) hypertrabeculation is increasingly recognized as a phenotype that may reflect physiological adaptation, particularly in athletes exposed to chronic overload, although its functional relevance remains uncertain. This study evaluated the prevalence of excessive trabeculation and its physiological correlation with LV remodeling. We conducted a single-center, cross-sectional study involving 320 Olympic-level athletes without cardiovascular disease. All underwent cardiac magnetic resonance (CMR). Hypertrabeculation was defined by the Petersen criteria. Athletes meeting these criteria were classified as hypertrabeculated and compared with non-hypertrabeculated matched for age, sex, and sport category. LV morphology, function, strain parameters, and hemodynamic forces (HDFs) were analyzed. Hypertrabeculation was identified in 9% of the cohort. No significant differences were observed between groups for training exposure (p = 0.262), body surface area (p = 0.762), LV volumes (end-diastolic volume indexed p = 0.397 end-systolic volume indexed p = 0.118), ejection fraction (p = 0.101), mass (p = 0.919), sphericity index (p = 0.419), myocardial wall thickness (p = 0.394), tissue characterization (T1 mapping p = 0.366, T2 mapping p = 0.833), global longitudinal strain (GLS p = 0.898), global circumferential strain (GCS p = 0.219), or HDFs. All values were within the normal range. In our cohort, LV hypertrabeculation, evaluated by CMR, was relatively common but not associated with structural or functional abnormalities, supporting its interpretation as a benign variant in asymptomatic athletes with normal cardiac function. Full article

The calculation of internal forces is a critical aspect in the design of statically indeterminate structures. Local trapezoidal loads, as a common loading configuration in practical engineering (e.g., earth pressure, uneven surcharge), make it essential to investigate how to compute the internal forces of statically indeterminate structures under such loads by using the displacement method. The key to displacement-based analysis lies in deriving the fixed-end moment formulas for local trapezoidal loads. Traditional methods, such as the force method, virtual beam method, or integral method, often involve complex computations. Therefore, this study aims to derive a general formula for fixed-end moments in statically indeterminate beams subjected to local trapezoidal loads by using the integral method, providing a more efficient and clear theoretical tool for engineering practice while addressing the limitations of existing educational and applied methodologies. The integral method is employed to derive fixed-end moment expressions for three types of statically indeterminate beams: (1) a beam fixed at both ends, (2) an an-end-fixed another-end-simple-support beam, and (3) a beam fixed at one end and sliding at the other. This approach eliminates the redundant equations of the traditional force method or the indirect transformations of the virtual beam method, directly linking boundary conditions through integral operations on load distributions, thereby significantly simplifying the solving process. Three representative numerical examples validate the correctness and universality of the derived formulas. The results demonstrate that the solutions obtained via the integral method align with software-calculated results, yet the proposed method yields analytical expressions for structural internal forces. Comparative analysis shows that the integral method surpasses traditional approaches (e.g., force method, virtual beam method) in terms of conceptual clarity and computational efficiency, making it particularly suitable for instructional demonstrations and rapid engineering calculations. The proposed integral method provides a systematic analytical framework for the internal force analysis of statically indeterminate structures under local trapezoidal loads, combining mathematical rigor with engineering practicality. The derived formulas can be directly applied to real-world designs, substantially reducing computational complexity. Moreover, this method offers a more intuitive theoretical case for structural mechanics education, enhancing students’ understanding of the mathematical–mechanical relationship between loads and internal forces. The research outcomes hold both theoretical significance and practical engineering value, establishing a solving paradigm for the displacement-based analysis of statically indeterminate structures under complex local trapezoidal loading conditions. Full article