Search Results (1,571)

Cryptochromes (CRYs) are a conserved class of blue light and near-ultraviolet light receptors that regulate diverse processes, including photomorphogenesis in plants. In the extreme Antarctic environment, ice algae endure intense UV radiation, prolonged darkness, and low temperatures, where cryptochromes play a vital role in light sensing and stress response. In this study, we cloned the complete open reading frame (ORF) of the cryptochrome gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L. Both in vivo and in vitro DNA photorepair assays showed that CiCRY-DASH1 effectively repairs cyclobutane pyrimidine dimer (CPD) and 6-4 photoproducts (6-4PPs) induced by UV radiation. Furthermore, deletion of the N-terminal and C-terminal loop regions, combined with activity assays, revealed that the C-terminal loop region plays a crucial role in photorepair activity. These findings elucidate the adaptive photorepair mechanisms of Antarctic microalgae and establish CiCRY-DASH1 as a valuable genetic resource. Specifically, the high catalytic efficiency and evolutionary robustness of the engineered variants position it as a promising marine bioactive agent for photoprotective therapeutics and a strategic target for constructing microbial chassis to enable sustainable drug biomanufacturing. Full article

(1) Background: Rubus geoides Sm., a native species of southern Patagonia, faces increasing threats due to climate change and anthropogenic land-use changes. Historically widespread, its distribution has become restricted by overgrazing, urban expansion, extractive industries, and direct harvesting from natural populations driven by interest in its nutraceutical potential since the first European settlements. (2) Methods: To assess its resilience and conservation prospects, we analyzed the morphological variability, genetic diversity, and population structure, complemented by species distribution modeling under past and future climate scenarios. (3) Results: Our findings reveal moderate genetic differentiation and private alleles in specific populations, alongside significant variation in flowering phenology. Paternity analysis indicates a tendency toward self-pollination, although this conclusion is constrained by the limited number of microsatellite markers employed. These results suggest post-glacial dispersal patterns and highlight the species’ potential for expansion under certain climate scenarios. (4) Conclusions: This study provides critical insights for biodiversity conservation and sustainable land management, directly aligned with the UN Sustainable Development Goals SDG 15 (Life on Land). Indirectly, this study contributes to SDG 2 (Zero Hunger) by highlighting the importance of threatened species that hold value for human consumption and food security. Land-use changes, particularly mining and green hydrogen industry settlements, may represent stronger limitations to species expansion than climate change itself. Full article

Sago pondweed (Stuckenia pectinata (L.) Börner) is a genetically and ecologically diverse submerged macrophyte, notable for its versatile reproductive characteristics, with a broad global distribution, excluding only the Arctic and Antarctic regions. This cosmopolitan species remains underexplored genetically in Lithuania compared to some other European regions. The aim of this study was to investigate the state and distribution of genetic diversity across Lithuanian river populations. We analyzed genetic variation in ten riverine populations using both simple sequence repeats (SSRs) and intersimple sequence repeats (ISSR). Genetic distances between genotypes and populations, as revealed by SSR markers, correlated with those determined using ISSR markers, confirming consistency across the two marker systems. STRUCTURE analysis revealed the presence of two distinct genotype pools. Our study demonstrated that the majority of genetic variation resides within populations, with an F_ST value of 0.212 (SSR) and a Φ_PT value of 0.352 (ISSR). These findings suggest high genetic differentiation among populations. The absence of a relationship between genetic diversity and hydrochemical or hydromorphological parameters at plant collection sites suggests that the population structure of this species is shaped primarily by evolutionary and/or demographic mechanisms, rather than by local environmental hydrochemical conditions. Overall, this study revealed high within-population genetic diversity and underlying genetic structure in S. pectinata populations across Lithuanian rivers. Full article

The Arctic is warming rapidly, with atmospheric rivers (ARs) amplifying ice melt, extreme precipitation, and abrupt temperature shifts. Detecting ARs in the Arctic remains challenging, because AR detection algorithms designed for mid-latitudes perform poorly in polar regions. This study introduces a regional deep learning (DL) image segmentation model for Arctic AR detection, leveraging large-ensemble (LE) climate simulations. We analyse historical simulations from the Climate Change in the Arctic and North Atlantic Region and Impacts on the UK (CANARI) project, which provides a large, internally consistent sample of AR events at 6-hourly resolution and enables a close comparison of AR climatology across model and reanalysis data. A polar-specific, rule-based AR detection algorithm was adapted to label ARs in simulated data using multiple thresholds, providing training data for the segmentation model and supporting sensitivity analyses. U-Net-based models are trained on integrated water vapour transport, total column water vapour, and 850 hPa wind speed fields. We quantify how AR identification depends on threshold choices in the rule-based algorithm and show how these propagate to the U-Net-based models. This study represents the first use of the CANARI-LE for Arctic AR detection and introduces a unified framework combining rule-based and DL methods to evaluate model sensitivity and detection robustness. Our results demonstrate that DL segmentation achieves robust skill and eliminates the need for threshold tuning, providing a consistent and transferable framework for detecting Arctic ARs. This unified approach advances high-latitude moisture transport assessment and supports improved evaluation of Arctic extremes under climate change. Full article

Kyphosids are prominent members of temperate and subtropical reef fish communities, though many species are not targeted due to their poor eating qualities. This study investigated the biology of the non-targeted zebrafish, Girella zebra, from waters off southern Western Australia. Frequent captures of small juveniles enabled confirmation of the formation of the first otolith zone, and marginal increment analysis verified the annual formation of opaque zones. Female G. zebra reached a maximum total length and age of 399 mm and 45 years, while males attained 431 mm and 36 years. Girella zebra exhibits a “square” form of growth, as do other Kyphosids, whereby rapid growth occurs during the first 6–8 years, followed by minimal growth throughout a long adult lifespan. Length and age at maturity were 290 mm and 6.7 years for females and 269 mm and 4.9 years for males. Spawning occurs from August to December, and large gonads in both sexes are indicative of spawning in large schools. Natural mortality (M) estimates (females: 0.10–0.15 year⁻¹; males: 0.12–0.18 year⁻¹) exceeded total mortality from catch curves, reflecting that commonly used M estimators are based on heavily fished stocks. This study provides rare biological data for a species unaffected by fishing. Full article

Benthic δ¹⁸O and δ¹³C values, as well as the mean grain size (MS) of sortable silt (SS), were used to construct the records of deep-water ventilation during the last 300 ka, at core GC02. This core is located at 4430 m water depth on the Madagascar basin near the Southwest Indian Ocean mid-ridge (SWIR). Decreased values of MS of SS reveal a weakened Antarctic Bottom Water (AABW) in the glacial periods, while increased values indicate enhanced AABW in the interglacial periods. The MS of SS record in GC02 exhibited a particularly good synchronization with a record based on the δ¹³C gradient between the North Atlantic and tropical Pacific Ocean, indicating that AABW is dominated by the overturning strength of the Atlantic meridional overturning circulation (AMOC), and showed a higher generation rate in the early stages of the glacial periods. A rapid reduction in δ¹³C occurred in MIS 2, 4, and 6; the MS values in GC02 and winter sea ice (WSI) also exhibited significant decreases and increases, respectively. By controlling the transport of ventilated water mass to deep waters and polar heat transport, in the Indian Ocean, both the change in AABW intensity and the Southern Ocean ice volume result from changes in the AMOC under the orbital modulation background. In the Southwest Indian Ocean, AMOC has a larger effect on ice volume during glacial periods, while its effect on AABW is relatively strong during interglacial periods. Full article

Antarctica’s aerodynamic roughness length (z_0m) is crucial for surface energy exchange and atmospheric modeling, but its remote sensing estimation remains challenging due to complex ice-surface conditions and limited observations. To address these challenges, this study establishes a z_0m retrieval framework derived from the Raupach model using Unmanned Aerial Vehicle (UAV), Reference Elevation Model of Antarctica (REMA), and Ice, Cloud, and land Elevation Satellite-2 (ICESat-2) datasets at three representative Antarctic sites. The results show that UAV benchmarks yield mean z_0m values of 0.009795, 0.011597, and 0.005203 m at Zhongshan Station, Great Wall Station, and Qinling Station, respectively. In experiments with ICESat-2 data, z_0m derived from ATL06 demonstrates accuracy comparable to that from ATL03 (RMSE = 7.45 × 10⁻⁶ m), with the best performance obtained at a 2 km window. Spatially, the agreement with UAV-derived z_0m decreases in the order: REMA > ICESat-2 (IDW-interpolated). The accuracy of REMA and ICESat-2 decreased with terrain complexity, from ice-free zones to the ice-shelf front and finally to the steep ice sheet margin. The elevation and slope variations emerge as dominant controls of z_0m spatial patterns. This study demonstrates the complementary strengths of UAV, REMA, and ICESat-2 datasets in Antarctic aerodynamic roughness estimation, providing practical guidance for data selection and methodology optimization. This study develops an improved z_0m retrieval method for Antarctica, clarifies the applicability and limitations of UAV, REMA, and ICESat-2 data, and provides methodological and data support for simulations of near-surface atmospheric parameters in Antarctica region. Full article

This study aimed to develop a dietary fiber-rich Antarctic krill composite shrimp surimi gel (AKSG) and to investigate the improvement effects of high protein (HP), high protein and dietary fiber (HPDF), and high dietary fiber (HDF) diet interventions on constipation behaviors and gut microbiota of mice. The results showed that the HPDF group significantly improved defecation in constipated mice, enhanced gastrointestinal peristalsis, and exhibited the most obvious effect on improving the colonic structure. The gut microbial analysis showed that the HPDF group increased the relative abundance of beneficial bacteria and improved the intestinal microbial environment of constipated mice. In addition, all groups effectively regulated the secretion of intestinal neurotransmitters. Inulin significantly increased the fecal water content by binding to water molecules, thus softening feces. Meanwhile, the addition of an appropriate amount of protein could further absorb water in the intestinal tract and relieve constipation. In conclusion, dietary fiber-rich AKSG might be a promising nutritious functional food for constipation relief. Full article

Antarctic ice shelves regulate ice-sheet discharge and global sea-level rise, yet their rapid retreat underscores the need for new, low-cost monitoring tools. We analyze ambient seismic noise recorded by seismometers on the Pine Island Glacier ice shelf to characterize wind-induced signals and detect persistent structural resonances. Power spectral analysis shows that wind sensitivity is strongly damped compared with bedrock sites: noise increases only 5–7 dB from 0 to 25 m s⁻¹ winds, versus a 42 dB increase at an inland bedrock station, reflecting the contrasted coupling environments of floating and grounded substrates. The horizontal-to-vertical spectral ratio (HVSR) spectrograms reveal two temporally stable peaks at ~2.2 Hz and ~4.3 Hz that persist across stations and remain independent of environmental forcing. Forward modeling indicates that these peaks correspond to S-wave resonances within the ice shelf. The inferred ice-water interface depth (~440 m) agrees with the Bedmap2 thickness estimate (466 m). This work demonstrates that HVSR provides an effective passive, single-station method for measuring ice shelf thickness. Full article

This study examines the occurrence of bacteria resistant to antibiotics and heavy metals in Terra Nova Bay, a coastal area of the Ross Sea in Antarctica that is increasingly recognised as vulnerable to human influence. During the 37th Italian Antarctic Expedition (2021–2022), researchers collected seawater, sediment, and fish samples from the notothenioid species Trematomus bernacchii to evaluate microbial resistance in an environment once considered largely pristine. Fifty heterotrophic bacterial isolates were obtained and tested against twenty-eight antibiotics, revealing a notable presence of multidrug resistance. These multidrug-resistant isolates were then assessed for their tolerance to eight heavy metal salts to understand whether resistance traits extended beyond antimicrobials. Twelve isolates showing resistance to both antibiotics and metals were selected for further genetic screening, targeting key resistance genes linked to tetracycline, vancomycin, sulphonamides, and other antimicrobial classes. The detection of multiple resistance genes in genera such as Pseudomonas, Pseudoalteromonas, and Psychrobacter indicates that both natural selective pressures and local, human-related contamination may be shaping resistance patterns in this region. Overall, the study demonstrates that even remote Antarctic marine ecosystems can host bacteria with complex resistance profiles. While these ecosystems are largely isolated, human activities such as scientific research, tourism, and the introduction of pollutants may contribute to the dissemination of antibiotic resistance genes, raising important ecological and potential public health considerations regarding the spread of resistance in polar environments. Full article

Cold-active lipolytic enzymes enable low-temperature biocatalysis, but remain underexplored in Antarctic actinomycetes. Here, we report the discovery and first-step characterization of a CALB-like cold-active lipolytic enzyme (PanLip) from Pseudonocardia antarctica. Sequence and structure analyses revealed a canonical α/β-hydrolase fold with a conserved Ser–Asp–His triad and short helical elements around the pocket reminiscent of CALB’s α5/α10 lid. Mature PanLip was expressed primarily as inclusion bodies in E. coli; an N-terminally truncation (PanLipΔN) improved solubility and PanLipΔN was purified by Ni–NTA. Far-UV CD confirmed a folded α/β architecture. PanLipΔN favored short-chain substrates (p-NPA, k_cat/K_M = 2.4 × 10⁵ M⁻¹·s⁻¹) but also showed measurable hydrolytic activity toward natural triglycerides, consistently with a lipase-family esterase. The enzyme showed an activity optimum near 25 °C and pH 8.0. The enzyme tolerated low salt (maximal at 0.1 M NaCl), mild glycerol, and selected organic solvents (notably n-hexane), but was inhibited by high salt, Triton X-100, and SDS. AlphaFold predicted high local confidence for the catalytic core; DALI placed PanLip closest to fungal lipases (AFLB/CALB). Temperature-series MD and CABS-flex indicated enhanced surface breathing and flexible segments adjacent to the active site—including a region topologically matching CALB α10—supporting a flexibility-assisted access mechanism at low temperature. Structure-based MSAs did not support a cold adaptation role for the reported VDLPGRS motif. Taken together, these findings position PanLip as a promising cold-active catalyst with CALB-like access control and potential for low-temperature biocatalysis. Full article

Convection associated with the leading mode of subseasonal variability of the tropical atmosphere, the Madden–Julian Oscillation (MJO), can excite Rossby wave trains that extend well into the extratropics and allow the MJO to modulate many components of the Earth system. To improve our understanding of teleconnections between the MJO and Antarctic sea ice, composite anomalies of daily change in sea ice concentration (ΔSIC) from 1989 to 2019 were binned by phase 0–20 days after an active MJO and compared to anomalies of surface air temperature, the meridional component of surface wind, and sea-level pressure. In May, ΔSIC anomalies were strongest in the Indian Ocean (IO) sector, 16 days after phase 8. There, a wavenumber-three pattern in sea-level pressure anomalies associated with the MJO resulted in anomalously poleward winds and warmer temperatures over the central and eastern IO that were collocated with anomalously negative ΔSIC. Furthermore, anomalously equatorward winds and colder temperatures in the western IO were collocated with anomalously positive ΔSIC. In July, ΔSIC anomalies were strongest in the Weddell Sea (WS) sector nine days after an active MJO in phase 2. There, a wavenumber-three pattern in sea-level pressure anomalies resulted in anomalously poleward winds and warmer temperatures over the western and central WS that were collocated with negative ΔSIC anomalies; anomalously equatorward winds and colder temperatures over the eastern WS were collocated with positive ΔSIC anomalies. In September, the largest ΔSIC anomalies were observed in the IO and WS sectors six days after an active MJO in phase 8. No meaningful modulation of sea ice anomalies was found after an active MJO in November or January. These results extend our understanding of teleconnections between the MJO and Antarctic sea ice on the subseasonal time scale. Full article

Plant–microbe interactions exert a significant influence on host stress responses; however, the molecular mechanisms underlying these effects remain inadequately understood. In this study, we characterize FaMAN8, an α-mannosidase from Fragaria × ananassa, to explore its role in adaptation to heat waves and water deficit, as well as its modulation by fungal endophytes. Transcriptomic analysis identified FaMAN8 as the sole α-mannosidase isoform highly conserved across reported sequences, with root-specific induction under conditions of heat stress, deficient irrigation, and endophytic colonization. Structural modeling revealed that FaMAN8 exhibits the canonical domain organization of glycoside hydrolase family 38 (GH38) enzymes, featuring a conserved catalytic architecture and metal-binding site. Molecular docking and dynamics simulations with the Man₃GlcNAc₂ ligand indicated a stable binding pocket involving key catalytic residues and strong electrostatic complementarity. MM-GBSA and free energy landscape analyses further supported the thermodynamic stability of the protein–ligand complex. Cavity analysis revealed a larger active site in FaMAN8 compared to its homolog JbMAN, suggesting broader substrate accommodation. Collectively, these findings identify FaMAN8 as a stress-responsive glycosidase potentially involved in glycan remodeling during beneficial root–fungus interactions. This work provides molecular insights into plant–microbe symbiosis and lays the groundwork for microbiome-informed strategies to enhance crop stress resilience. Full article

Antarctica harbors some of the most extreme ecosystems on earth, where only two vascular plants persist. The native grass Deschampsia antarctica provides a model for plant–microbe interactions under intense abiotic stress. We present the first multi-compartmental and multi-kingdom characterization of bacterial and fungal communities associated with D. antarctica across three South Shetland Islands. Metabarcoding revealed strong compartmentalization: the rhizosphere displayed the highest richness and complex bacterial–fungal networks; the root endosphere showed intermediate diversity with keystone taxa such as Rhizobiales and Streptomyces; and the leaf endosphere was simplified, dominated by stress-tolerant taxa including Pseudomonas and Helotiales. Despite marked soil heterogeneity, phosphorus enrichment at Admiralty Bay, base cations at Coppermine Cove, and iron at Byers Peninsula, a conserved core (20 bacterial and 5 fungal genera) persisted, mainly cold-adapted saprotrophs and plant-associated taxa. Fungal assemblages were more responsive to soil chemistry, with site-specific enrichments such as Zymoseptoria and Herpotrichia. Overall, D. antarctica holobionts exhibited a dual strategy: conserved microbial backbones confer stability, while localized assemblages shaped by soil chemistry and geography enhance adaptability. Together, these findings provide one of the most integrative characterizations of the D. antarctica holobiont to date, revealing how conserved and adaptive microbial components support plant resilience under extreme Antarctic conditions and offering valuable insights for predicting biological responses to ongoing climate change. Full article

Several Level-2 solutions for the GRACE(-FO) gravity field exist. We compare five of these solutions using a mascon inversion method to estimate gridded mass change in Antarctica from gravity field grids at orbit height. We compare the mass change for all of Antarctica, as well as for 27 drainage basins. All five solutions show consistent negative mass trends for the period between April 2002 and January 2025 and show a mass accumulation in the East Antarctic basins 12 and 13 during the years 2021, 2022, and the beginning of 2023 but also a rapid mass loss starting in May 2023. While there are regional differences, the error from the Glacial Isostatic Adjustment model exceeds the differences between the solutions looked at in this study. Full article