Search Results (127)

Vibration-based sensing systems for deployed industrial fault diagnosis often face incremental fault classes, changing degradation stages, and limited permission to retain historical sensor streams. Static fault classifiers are therefore insufficient for online maintenance settings in which a model must learn new sensor-observed states while preserving previous diagnostic knowledge under a bounded memory budget. This paper proposes ASBR-CL, an adaptive stage-aware balanced replay framework for continual fault diagnosis under a fixed exemplar-memory budget. ASBR-CL combines dataset-adaptive exemplar memory, balanced replay between current data and retained exemplars, and a conditional validation checkpoint module that is enabled only when it improves balanced stage recognition. Experiments on SEU-enhanced92, the 92-dimensional feature construction for SEU, and XJTU-bearing23, the 23-dimensional feature construction for XJTU-SY, compare ASBR-CL with DGGN/MFF same-backbone continual-learning baselines and XJTU imbalance-aware variants under five random seeds and $K=100$ training exemplars. On SEU, the selected ASBR-CL setting reports $97.49\pm 1.18$ Average Accuracy, $90.37\pm 4.53$ Final Accuracy, $90.37\pm 4.53$ Macro Recall, and $11.50\pm 5.25$ Average Forgetting. On XJTU, the conservative ASBR-CL-BoundedVal-K100 setting is not a universal Final Accuracy winner: DGGN-ER reaches a slightly higher Final Accuracy ($89.52\pm 2.68$ versus $89.05\pm 2.48$). The ASBR-CL evidence instead lies in balanced recognition and retention, with Macro Recall $75.00\pm 2.00$, Macro-F1 $67.66\pm 3.83$, and Average Forgetting $20.39\pm 4.89$, compared with DGGN-ER at $63.94\pm 7.50$, $55.48\pm 12.82$, and $41.11\pm 13.61$. Additional validation-resource, RMS-derived stage-definition, and imbalance-aware baseline analyses show that the revised XJTU claim should be framed as more stable Macro Recall, Macro-F1, and forgetting control under memory-limited continual diagnosis, not as superiority on every accuracy metric. Full article

Glutamine-Fructose-6-Phosphate Transaminase 1 (GFPT1), the rate-limiting enzyme of the hexosamine biosynthetic pathway (HBP), provides the UDP-N-acetylglucosamine (UDP-GlcNAc) required for protein glycosylation. Biallelic mutations in GFPT1 cause congenital myasthenic syndromes (GFPT1-CMS), yet the molecular mechanisms linking impaired glycosylation to skeletal muscle dysfunction remain incompletely understood. Here, we combine cellular models of inducible Gfpt1 knockdown and a skeletal muscle-specific Gfpt1 knockout mouse (Gfpt1^Tm1d/Tm1d) with whole-cell proteomics, immunoblot studies and secretomics to define glycosylation-dependent defects in intracellular trafficking, ER stress signaling and autophagy. Global proteomic profiling of Gfpt1-deficient myoblasts revealed marked downregulation of protein trafficking pathways and impaired secretion of key muscle cargo proteins, including serglycin (Srgn). Loss of GFPT1 reduced both high-molecular-weight glycosylated serglycin and its core protein, accompanied by intracellular retention and decreased secretion. These trafficking defects coincide with robust activation of the unfolded protein response (UPR), evidenced by increased Xbp1 expression and accumulation of spliced Xbp1s across pharmacologic, cellular, and mouse models of GFPT1 deficiency. Converging evidence from proteomics, immunoblotting, and immunofluorescence demonstrated impaired autophagy, including increased LC3-II accumulation, elevated p62/Sqstm1 levels, and enhanced p62-positive puncta in both Gfpt1-deficient C2C12 myoblasts and skeletal muscle. Soluble/insoluble fractionation further confirmed p62 accumulation, indicating defective autophagic flux and buildup of aggregated cargo. Together, these findings identify a glycosylation-dependent failure in protein trafficking that triggers ER stress, UPR activation, and autophagy impairment in Gfpt1-deficient skeletal muscle. This mechanistic cascade provides a unifying explanation for muscle pathology in GFPT1-CMS and suggests that restoring glycosylation or improving proteostasis may represent viable therapeutic approaches. Full article

Hydrothermal carbonization (HTC) of wet biomass faces a fundamental tradeoff between higher heating value (HHV) and energy recovery (ER), where conditions that enhance carbon densification often reduce solid-phase energy retention. This study investigates whether co-biomass selection combined with citric acid (CA) catalysis can overcome this tradeoff in HTC of water hyacinth (WH), an invasive aquatic feedstock. WH was co-processed with wheat straw (WS), rice husk (RH), and chicken manure (CM) at 240–270 °C, with CA-assisted experiments performed at 240 °C. Individual feedstock HTC confirmed the HHV–ER tradeoff, and co-HTC without catalysis failed to resolve it. CA addition improved carbon densification but reduced ER when applied to WH alone. The WH–CM–CA system uniquely achieved a concurrent HHV of 21.3 MJ kg⁻¹ and ER of 95.8%, with synergistic effects of 50.0% and 29.7%, respectively. FTIR and elemental analysis indicated that Maillard-type condensation between WH-derived sugars and CM-derived amino acids drove preferential solid-phase carbon retention. These findings demonstrate that resolving the HHV–ER tradeoff requires coupling CA catalysis with biochemical complementarity between carbohydrate-rich and protein-rich feedstocks. This approach provides a practical route for hydrochar production with high energy density and recovery for waste-to-energy applications, supporting circular and low-carbon valorization of invasive aquatic biomass and livestock waste streams. Full article

Universal adhesives (UAs) exhibit considerable versatility; however, no single commercial product has attained recognition as a clinical gold standard. This study evaluated the functional performance, retention, and marginal integrity of various UA brands in non-carious cervical lesion (NCCL) restorations and examined the effects of different adhesion strategies. A search of electronic databases was conducted for randomized clinical trials (RCTs) published between 2015 and 2025. Only RCTs that assessed the retention and marginal integrity of UAs with follow-ups of 18–48 months, using the USPHS/FDI criteria, were included. This review was registered with PROSPERO (CRD420251026490) and adhered to PRISMA 2020 and PICOS guidelines. Risk of bias was evaluated using the RoB 2 tool; statistical significance was defined as p < 0.05. Of 251 records screened, 23 met the eligibility criteria, resulting in the inclusion of 21 RCTs. Sixteen UA brands exhibited no clear differences in performance outcomes. Etch-and-rinse (ER) and selective enamel-etching (SEE) strategies achieved higher retention rates (median up to 100%; USPHS, p < 0.001), while the self-etch (SE) approach demonstrated lower and more variable retention (median 87.0%). Marginal integrity remained consistently high across all strategies (median 100%; p > 0.05). Although ER and SEE strategies significantly enhance long-term retention, no UA brand showed consistent superiority to be considered a gold standard. Full article

Water age reflects water sources, storage, and pathways, and regulates the solute retention and dissolution associated with biogeochemical processes, highlighting its hydrological and ecological importance. However, accurate water age estimation in tracer-aided models depends heavily on the quality and spatio-temporal resolution of precipitation isotopic signals. This study investigates how distributed rainfall δ²H signals affect the simulation of young water fraction (F_yw) via the Storage Age Selection (SAS) model in topographically complex subtropical mountain catchments. Eight precipitation δ²H scenarios were generated using two temporal approaches (stepwise and sinewave) and four spatial interpolation methods: (1) raw data, (2) reversed effective recharge elevation method (rERE), (3) linear regression with elevation (ER), and (4) regression-kriging (RK). Later on, the time-variant SAS model was calibrated against observed stream water δ²H collected from the year 2022 to the year 2024. Results show that the SAS model consistently produced similar F_yw estimates for catchments (8%~40%) across all eight scenarios, demonstrating strong robustness to input uncertainty and validating the dominant role of catchment characteristics in regulating water age. The combined stepwise temporal and rERE spatial approach provided better agreement with observed stream δ²H, particularly in the eastern, steeper catchments, yielding superior model efficiency along with better constrained uncertainty. This study highlights the sensitivity of age-tracking models to precipitation isotopic inputs and provides practical guidance for selecting an interpolation strategy in data-limited mountainous environments. Full article

Alpha-1 antitrypsin deficiency (AATD) is an inherited disorder characterized by intracellular retention of mutant Z (Pi*Z) alpha-1 antitrypsin (AAT) within hepatocytes, resulting in progressive liver disease. Currently, no approved pharmacological therapies exist for AATD-associated hepatic injury. Emerging preclinical evidence indicates that inhibition of mammalian target of rapamycin (mTOR) ameliorates liver pathology in AATD; however, the status of mTOR activity and its regulatory mechanisms under Pi*Z AAT-induced cellular stress remains incompletely understood. In this study, we investigated alterations in mTOR signaling and its upstream regulatory pathways using a gene-edited human hepatocyte model harboring the Pi*Z mutation (Huh7.5Z cells) and a Pi*Z AAT transgenic mouse model. Attenuation of mTORC1 activity was observed in both cellular and murine Pi*Z models. In vitro analyses demonstrated activation of AMP-activated protein kinase (AMPKα), a key inhibitory regulator of mTORC1, accompanied by paradoxical activation of Akt and the unfolded protein response (UPR) branch ATF6α. Pharmacological inhibition of mTOR significantly reduced intracellular Pi*Z AAT accumulation, alleviated ER stress, and suppressed apoptotic signaling through enhancement of autophagy. These findings reveal that hepatocytes adapt to Pi*Z AAT-induced stress through coordinated regulation of mTOR by AMPK, Akt, and ATF6α pathways. This study provides mechanistic insight into metabolic and stress-response signaling in AATD and identifies mTOR modulation as a promising therapeutic strategy for AATD-associated liver disease. Full article

The microstructure and mechanical properties of the joint of a novel Al-Mg-Zn-Er-Zr alloy fabricated by multi-pass MIG welding using ER5E61 filler wire were investigated first. The results show that multi-pass MIG welding induces heterogeneous grains in the weld metal: equiaxed grains, columnar grains, and cover-pass feather-like grains. The weld metal exhibits coarse grains (45.81 ± 19.68 μm), a high proportion of high-angle grain boundaries (83.3%), and a low dislocation density compared with the base metal. The joint achieves 316 MPa ultimate tensile strength, 10.5% elongation, and 0.80 joint efficiency with minimum hardness (77.2 HV) in the weld metal. Strengthening mechanism analysis reveals that joint softening mainly stems from the disappearance of deformed structure, reduced dislocation density, and the coarsening and reduction in Al3(Er, Zr) nanophases. Diffuse precipitation of the Al3(Er, Zr) nanophases (19.61 nm, 0.53%) under multi-pass MIG welding compensates for the softening of the welded joint, leading to the retention of high tensile strength despite marked hardness loss, thus demonstrating effective strength preservation. Full article

Background/Objectives: Non-carious cervical lesions (NCCLs) are common defects in adults that can lead to dentin hypersensitivity and aesthetic concerns, for which composite resin restorations currently represent the gold standard of care. However, evidence regarding the long-term clinical superiority of high-filled injectable composites and Er,Cr:YSGG laser-based cavity preparation remains limited. The present study aimed to compare the 1-year clinical performance of two different surface preparation protocols (Er,Cr:YSGG laser vs. conventional bur preparation with phosphoric acid etching) and two composite resin types (high-filled injectable vs. conventional paste-type) in the restoration of NCCLs. Methods: In this prospective, split-mouth, randomized controlled clinical trial, a total of 168 NCCLs in 27 patients were restored. Lesions were randomly allocated to four groups according to the combination of surface preparation (Er,Cr:YSGG laser or phosphoric acid etching) and high-filled injectable composite (G-ænial Universal Injectable) or paste-type composite (G-ænial Anterior). The same universal adhesive system was used in all cases. Clinical evaluations were performed by a blinded examiner at 1 week, 6 months, and 12 months, using the FDI World Dental Federation criteria. Results: At the 1-year follow-up, 25 patients and 150 restorations were available for evaluation, corresponding to a recall rate of 98.22%. High clinical acceptability was observed in all groups with respect to aesthetic, functional, and biological parameters. Retention was 100% in the acid-etched paste-type composite group and ranged from 94.7% to 97.4% in the remaining groups, with no statistically significant differences among groups (p > 0.05). A transient increase in postoperative sensitivity was detected in the laser groups at the 1-week evaluation (p = 0.026); however, sensitivity scores declined to zero in all groups at 6 months and 1 year. Conclusions: High-filled injectable composites demonstrated 1-year clinical performance comparable to that of conventional paste-type composites in the restoration of NCCLs. Er,Cr: YSGG laser-based cavity conditioning produced outcomes similar to conventional phosphoric acid etching with respect to retention, marginal adaptation, and biological compatibility. The early increase in laser-related postoperative sensitivity was transient and did not compromise long-term clinical success. Taken together, the ease of application and favorable clinical performance of injectable composites indicate that these materials constitute a reliable alternative for the restoration of non-carious cervical lesions. Full article

Glucocorticoid (GC) residues present in aquatic products raise food safety concerns, as their chronic dietary intake may pose potential risks of endocrine and metabolic disruption. For the first time, a sensitive and reliable liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed and validated herein for the simultaneous determination of 12 GCs residues, including critical isomeric pairs and acetate ester derivatives, in a variety of aquatic foods, employing deuterated isotopic internal standards. Key optimizations included using a pentafluorophenyl column for effective isomer separation, a synergistic extraction system for high recovery, and QuEChERS purification to mitigate matrix effects. The method exhibited excellent linearity (r² > 0.996) and high accuracy (recoveries 97.3–99.3%), and the intra- and inter-day precision values were below 3% in five representative aquatic matrices, with a limit of detection (LOD) and a limit of quantification (LOQ) of 0.5 μg/kg and 0.75 μg/kg, respectively. Animal experiments confirmed the in vivo retention of acetate derivatives, justifying their inclusion in monitoring. Real sample analysis of 18 market samples revealed the presence of cortisone and hydrocortisone in 17 samples. This represents the first reported LC-MS/MS method that provides a sensitive, reliable tool for regulatory monitoring of GC residues in diverse aquatic products, thereby supporting food safety assurance. Full article

The separation of rare earth elements (REEs) with similar chemical properties remains a relevant challenge today, most often addressed using liquid–liquid and solid-phase extraction with various chelating agents. Excellent complexing agents for REEs are 1,3-diketones and their analogs. We have for the first time proposed a method for preparing a material consisting of a covalently immobilized 3-acyl-4-hydroxycoumarin ligand on silica. For its synthesis, we employed a strategy based on the “click” reaction of 3-azidopropyl silica with a propargyl-containing coumarin–chalcone conjugate—this approach is the most tolerant and does not affect the coordinationally active fragment of the ligand. The material was characterized by thermal analysis, IR spectroscopy, and ¹³C NMR. The potential of the synthesized material for REE preconcentration was demonstrated at pH 5–5.5: high extraction efficiency for Gd(III), Dy(III), Er(III), Eu(III), Sm(III), and Yb(III) was observed, with fast adsorption kinetics (30 min) and extraction degrees of ~98%. Under unified conditions of static and dynamic extraction for Gd(III), Dy(III), Er(III), Eu(III), Sm(III), and Yb(III), affinity series toward the surface were obtained as a function of the distribution coefficient. It was shown that 10-fold molar excesses of Fe(III), Al(III), Cu(II), Ni(II), and Co(II) allow retention of more than 95% extraction for Dy(III) and Er(III). After adsorption of Dy(III) and Er(III), shifts in the carbonyl group absorption bands are visible in the IR spectra of the material, indicating a chelating mechanism of sorption. Additional studies are required for implementation in analytical and preparative REE separation schemes; however, preliminary data show that the material is a highly active adsorbent. Full article

Offensive-language detection systems that perform well at a given point in time often degrade as linguistic patterns evolve, particularly in dialectal Arabic social media, where new terms emerge and familiar expressions shift in meaning. This study investigates temporal linguistic drift in Saudi-dialect offensive-language detection through a systematic evaluation of continual-learning approaches. Building on the Saudi Offensive Dialect (SOD) dataset, we designed test scenarios incorporating newly introduced offensive terms, context-shifting expressions, and varying proportions of historical data to assess both adaptation and knowledge retention. Eight continual-learning configurations—Experience Replay (ER), Elastic Weight Consolidation (EWC), Low-Rank Adaptation (LoRA), and their combinations—were evaluated across five test scenarios. Results show that models without continual-learning experience a 13.4-percentage-point decline in F1-macro on evolved patterns. In our experiments, Experience Replay achieved a relatively favorable balance, maintaining 0.812 F1-macro on historical data and 0.976 on contemporary patterns (KR = −0.035; AG = +0.264), though with increased memory and training time. EWC showed moderate retention (KR = −0.052) with comparable adaptation (AG = +0.255). On the SimuReal test set—designed with realistic class imbalance and only 5% drift terms—ER achieved 0.842 and EWC achieved 0.833, compared to the original model’s 0.817, representing modest improvements under realistic conditions. LoRA-based methods showed lower adaptation in our experiments, likely reflecting the specific LoRA configuration used in this study. Further investigation with alternative settings is warranted. Full article

The intracellular retention of misfolded extracellular matrix proteins is a common disease mechanism in various rare skeletal diseases. This discovery has driven the study of ER stress and the unfolded protein response (UPR) as a promising therapeutic target in several skeletal dysplasias. In the case of COL10A1 mutations, targeting the UPR resulted in a clinical trial of the repurposed drug carbamazepine; however, for other closely related skeletal disorders, treatment with carbamazepine was ineffective, indicating the need for suitable markers for in vitro screenings of potential drug treatments. Mutations in cartilage oligomeric matrix protein (COMP), a cartilage structural protein, cause both multiple epiphyseal dysplasia (MED) and pseudoachondroplasia (PSACH); together referred to as the COMPopathies, which result from the intracellular retention of mutant COMP to varying degrees. In contrast to other closely related skeletal disorders, caused by mutations in cartilage structural proteins, the involvement of the UPR is less clear, and so far, no common COMPopathy marker has been identified. Here, using cell models of COMPopathies, we identified MMP9 upregulation as a common feature of six pathogenic COMP variants that do not induce a prominent UPR. We further show that the archetypal p.V194D matrilin-3 MED variant (which causes MED) does not induce MMP9 expression, suggesting that MMP9 upregulation could serve as a specific marker of COMPopathies in vitro. Full article

This systematic review aimed to evaluate the effect of laser surface treatment on the bond strength of adhesive restorative materials to primary teeth. A comprehensive literature search was conducted in PubMed, Scopus, Embase, Web of Science and WorldCat up to July 2025 using the keywords primary teeth, deciduous teeth, milk teeth, laser, adhesion, bond strength. Twenty-six studies met the inclusion criteria, including 22 shear bond strength (SBS), three microtensile bond strength (µTBS) and one microshear bond strength (µSBS) investigations. Most studies evaluated erbium lasers (Er:YAG, Er,Cr:YSGG), while fewer assessed diode, Nd:YAG or KTP devices. In dentin, erbium lasers at low-to-moderate energy levels consistently produced smear-free, micro-retentive surfaces with open tubules and bond strengths comparable to bur-prepared controls. High-energy irradiation, however, frequently caused microcracks, tubule collapse and reduced adhesion. In enamel, phosphoric acid etching remained the most effective conditioning method, although combined laser–acid pretreatment often improved bonding of sealants and composites. Material-dependent effects were also evident: resin composites generally outperformed glass ionomers, hybrids and bioactive restorative materials. Phosphoric acid etching remains indispensable for enamel conditioning, while dentin benefits from carefully controlled erbium laser irradiation in combination with suitable adhesive systems. Full article

Amid the combined pressures of global carbon-reduction in architecture and the imperative of cultural heritage conservation, new courtyard-style buildings in hot-summer and cold-winter regions face a dual challenge of reconciling historical morphological constraints with contemporary comfort requirements. At the same time, the prevailing energy-efficiency codes in these regions, emphasizing high airtightness and strong insulation, have revealed shortcomings such as poor indoor air quality and insufficient summer ventilation. This study takes the Lu Residence in Dongyang, Jinhua, Zhejiang Province, as the primary case. It systematically examines the coupling mechanisms between the geometric configurations of transitional space in traditional courtyard dwellings and their environmental physical parameters using field surveys, multi-parameter environmental monitoring, and computer simulations. The results identify the optimal orientations and geometric parameters that balance summer ventilation with winter thermal buffering in hot-summer and cold-winter regions. The primary conclusions of this research are as follows: (1) The optimal orientation for axial buildings lies between 15° west of south and 15° east of south, as well as 30–60° east or west of south, with an angle of 45–60° in relation to the prevailing annual wind direction for all buildings. (2) The optimal height-to-width ratio of the courtyard is less than 1:2.5, while the range of the length-to-width ratio extends from 1:0.5 to 1:0.7. (3) The optimal eave depth varies from 900 to 1500 mm, effectively balancing winter heat retention and summer shading; however, a depth of 2400 mm is primarily advantageous for shading purposes. Furthermore, these findings are applied to the design of a new guesthouse within the conservation area of the Xu Zhen Er Gong Ancestral Hall in Yongkang, establishing a climate–geometry matching mechanism for transitional spaces. The study demonstrates that transitional space can serve as effective passive regulators, offering a scientific and sustainable pathway for the adaptive continuation of traditional courtyard architecture. Full article

Lysolecithin, characterized by its superior emulsifying and stabilizing properties, facilitates nutrient absorption and is extensively utilized in aquatic feed formulation. Nevertheless, its precise function in shrimp nutrition and physiology remains inadequately understood. This study aimed to evaluate the feasibility and optimal dosage of replacing 2% soybean lecithin with varying levels of soybean lysolecithin (0–2%) in the diet of Litopenaeus vannamei. Growth performance, antioxidant indices, and lipid metabolism were assessed. The results demonstrated that dietary supplementation with 0.1% lysolecithin had the best growth performance, significantly improved lipid retention and apparent crude fat digestibility, while reducing malondialdehyde (MDA) levels in the hepatopancreas and alleviating endoplasmic reticulum (ER) stress. The 0.1% group also exhibited better hepatopancreatic tissue structure and lipid metabolic homeostasis. In contrast, higher inclusion levels (≥1.5%) led to increased lipid accumulation and enhanced activities of lipid metabolic enzymes but were associated with a risk of oxidative stress and less favorable tissue morphology. No significant differences in antioxidant enzyme activities were observed among groups. It is hypothesized that lysolecithin may regulate lipid metabolism and homeostasis via the Ca²⁺/CaMKKβ/AMPK signaling pathway; further studies are required to confirm this mechanism. In conclusion, 0.1% soybean lysolecithin is recommended as the optimal dietary level for L. vannamei, supporting its feasibility as a substitute for 2% soybean lecithin in shrimp feed. Full article