Search Results (524)

In the case of a notifiable animal disease like salmonellosis, manure is contaminated and must be disinfected. This can be performed using heat measures, chemical disinfectants, or long-term storage. All these measures bring along severe economic, ecological, and logistical problems. The aim of this study was to evaluate lactic acid fermentation (LAF) as an alternative disinfection method. Fermentation was started by adding a carbohydrate source to the manure and creating anaerobic conditions. For testing, cattle manure was enriched with different carbohydrate (CHO) sources and spiked with Salmonella Typhimurium (S. Ty.). The samples were incubated at 10 °C and 21 °C for 111 days (Exp1) and at 21 °C for 50 days (Exp2). The microbial shift was determined using cultural methods and MALDI-TOF. Both the change in pH and Enterococcus spp. were tested as suitable indicators. The results showed the different suitability of the selected CHO for hygienization by LAF. Using squeezed oat as an additive, S. Ty was reduced to below the detection limit under both temperature conditions within 21 days and 14 days. Additional saccharose decreased the reduction time. This study showed that LAF is a valuable alternative for disinfecting cattle manure in the case of bovine salmonellosis. Using this method, both manure and feed residues can be treated in one approach and afterwards be used as fertilizer. Full article

Modern networked industrial applications often require low-latency communication. Some applications evolve over time, however, are tied to yet existing infrastructures, like power grids spanning across large areas. For instance, medium voltage direct current (MVDC) grids are evolving to a promising alternative to traditional medium voltage alternating current (MVAC) grids due to their efficiency and suitability for novel use cases like electric mobility. MVDC grids, however, require an active control and fault handling strategy. Some strategies demand for a continuous state exchange of the converter substations via a low-latency communication channel with less than 1 millisecond. While some communication approaches for MVDC grids are described in the literature, none of them is inherently designed to be secure. In this paper, we present a protocol for ultra-low-latency secure state exchange (PULLSE) based on conventional non-deterministic Ethernet and AES-GCM. We chose Ethernet in order to not limit the approaches usability in terms of hardware requirements or communication patterns. PULLSE is designed to prevent traffic eavesdropping, replay, and manipulation attacks. Full article

Target detection is a cornerstone task in hyperspectral image processing but faces significant challenges due to domain gaps. While statistical detectors like Constrained Energy Minimization (CEM) and Adaptive Cosine Estimator (ACE) are not prone to learned biases, in practice they still suffer from mismatches between the reference target spectrum and the spectral characteristics of the target in the test scene. We propose Test-time Adaptive Spectrum Refinement (TASR), a novel framework addressing this problem. TASR operates in an interpretable, lightweight, data-efficient manner, requiring only a single labeled source image of the target material. At test time, TASR dynamically refines the target spectrum to better align with the spectral properties of the test scene. This adaptive refinement enables detectors to effectively handle data with spectral variations, bridging the gap between the source and test spectra. To validate TASR, we conduct extensive experiments on established benchmarks and introduce a new dataset—ShadySunnyDiffuse (SSD)—which explicitly tests detector robustness to naturally occurring illumination changes. We further demonstrate the method’s versatility by applying it to camouflage detection and show compatibility with multiple statistical detectors. Our results establish TASR as a state-of-the-art approach in domain-adaptive hyperspectral target detection and target spectrum management. Full article

Current agentic AI frameworks such as LangGraph and AutoGen simulate autonomy via sequential prompt chaining but lack true multi-agent coordination architectures. These systems conflate semantic reasoning with orchestration, requiring LLMs at every coordination step and limiting scalability. By contrast, TB-CSPN (Topic-Based Communication Space Petri Net) is a hybrid formal architecture that fundamentally separates semantic processing from coordination logic. Unlike traditional Petri net applications, where the entire system state is encoded within the network structure, TB-CSPN uses Petri nets exclusively for coordination workflow modeling, letting communication and interaction between agents drive semantically rich, topic-based representations. At the same time, unlike first-generation agentic frameworks, here LLMs are confined to topic extraction, with business logic coordination implemented by structured token communication. This hybrid architectural separation preserves human strategic oversight (as supervisors) while delegating consultant and worker roles to LLMs and specialized AI agents, avoiding the state-space explosion typical of monolithic formal systems. Our empirical evaluation shows that TB-CSPN achieves 62.5% faster processing, 66.7% fewer LLM API calls, and 167% higher throughput compared to LangGraph-style orchestration, without sacrificing reliability. Scaling experiments with 10–100 agents reveal sub-linear memory growth (10× efficiency improvement), directly contradicting traditional Petri Net scalability concerns through our semantic-coordination-based architectural separation. These performance gains arise from the hybrid design, where coordination patterns remain constant while semantic spaces scale independently. TB-CSPN demonstrates that efficient agentic AI emerges not by over-relying on modern AI components but by embedding them strategically within a hybrid architecture that combines formal coordination guarantees with semantic flexibility. Our implementation and evaluation methodology are openly available, inviting community validation and extension of these principles. Full article

Sporotrichosis is a zoonotic fungal infection with increasing incidence in the Brazilian Amazon, primarily affecting domestic cats and posing risks to human health. This study characterized the clinical and epidemiological profiles of 29 feline sporotrichosis cases in Manaus and optimized molecular diagnostic methods for Sporothrix species identification. Most affected cats were young (86.2% aged 1–3 years), male (82.7%), and free-roaming or semi-indoor (44.8% each), frequently presenting cutaneous lesions localized at the nasal planum (23.3%), ears (7%), eyes (2.3%) and other facial areas (18.6%). Three DNA extraction methods were compared; the phenol–chloroform protocol yielded the highest DNA concentration and purity, and ITS1–ITS4 primers showed an adequate sensibility for PCR detection. In silico RFLP profiles using common restriction enzymes showed limited discriminatory power among Sporothrix species. ITS sequencing of four high-quality amplicons confirmed all isolates as Sporothrix brasiliensis. Antifungal susceptibility testing of all isolates revealed geometric mean MICs of 0.25 µg/mL for ketoconazole, 0.57 µg/mL for itraconazole, 7.27 µg/mL for amphotericin B, and 64 µg/mL for fluconazole, respectively. These findings provide clinical, molecular, and therapeutic information supporting the diagnosis and surveillance of feline sporotrichosis in the Amazon, reinforcing the need for ongoing veterinary and public health monitoring. Full article

Background/Objectives: Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, remains a major neglected tropical disease, with over six million cases concentrated, primarily in Latin America. Despite decades of research, treatment continues to rely on two outdated drugs—benznidazole and nifurtimox—both of which exhibit limited efficacy and are associated with severe side effects. In this context, drug repurposing presents a promising strategy to accelerate the development of safer and more effective therapies. Nitroxoline, a hydroxyquinoline compound widely used in Europe to treat bacterial urinary tract infections, has recently garnered attention for its broad-spectrum antimicrobial and anticancer activities. This study evaluated the antitrypanosomal potential of nitroxoline against both epimastigote and intracellular amastigote forms of T. cruzi, demonstrating significantly greater efficacy than benznidazole. Methods: In addition to its antiparasitic activity, we investigated the mechanism of parasite death and found that nitroxoline induces hallmarks of programmed cell death, including chromatin condensation, mitochondrial membrane depolarization, ATP depletion, reactive oxygen species accumulation, and increased membrane permeability. These cellular events are critical for minimizing host tissue inflammation and suggest a safer therapeutic profile. Results: The nitroxoline was shown to induce greater activity than the reference treatment, benznidazole, in addition to triggering events related to apoptotic or silent cell death. Conclusions: Given its established clinical use and favorable safety data, nitroxoline emerges as a strong candidate for further investigation as a repurposed treatment for Chagas disease. Future work should focus on in vivo efficacy, pharmacokinetics, and drug delivery strategies to enhance systemic bioavailability. Full article

Mycoplasmas are known respiratory pathogens in tortoises, but few studies exist in snakes. To better understand the correlation with clinical signs and co-infections, samples from mycoplasma-positive snakes with and without clinical respiratory disease were analyzed. Oral swabs from 15 snakes (pythons n = 12, boas n = 3) were examined using polymerase chain reaction (PCR) and third-generation sequencing (TGS). Additionally, mycoplasma isolation assays were performed. Pathogens detected by PCR included Mycoplasmas (15/15, 100%), serpentoviruses (9/15, 60%), and Chlamydia sp. (2/15, 13%); those detected by TGS included Mycoplasmas (14/15, 93%), serpentoviruses (10/15, 67%), Chlamydia sp. (1/15, 7%), and 15 different bacterial species. Sequencing of the mycoplasma PCR products revealed a close genetic relationship to Mycoplasmopsis agassizii. TGS identified genetically distinct mycoplasmas and three different serpentoviruses. While mycoplasmas could not be successfully propagated, Brucella intermedia comb. nov. was identified in eight cultures. Respiratory disease in snakes is often multifactorial, involving various pathogens and environmental influences. This study demonstrates that comprehensive diagnostics are essential for understanding disease processes in snakes and improving the detection of diverse pathogens. Further research is needed to improve laboratory diagnostics for infectious diseases in reptiles and to better understand the roles of various pathogens in respiratory diseases in snakes. Full article

This work investigates the impact of an internal electric field on the annihilation characteristics of positrons implanted in a $180\left(10\right)\mathrm{nm}$ SiO₂ layer of a Metal-Oxide-Silicon (MOS) capacitor, using Positron Annihilation Lifetime Spectroscopy (PALS). By varying the gate voltage, electric fields up to $1.72\mathrm{MV}/\mathrm{cm}$ were applied. The measurements reveal a field-dependent suppression of positronium (Ps) formation by up to $64\%$, leading to an enhancement of free positron annihilation. The increase in free positrons suggests that vacancy clusters are the dominant defect type in the oxide layer. Additionally, drift towards the SiO₂/Si interface reveals not only larger void-like defects but also a distinct population of smaller traps that are less prominent when drifting to the Al/SiO₂ interface. In total, by combining positron drift with PALS, more detailed insights into the nature and spatial distribution of defects within the SiO₂ network and in particular near the SiO₂/Si interface are obtained. Full article

The thermal stability of carbon fiber-reinforced plastic (CFRP) materials is constrained by the low thermal conductivity of its polymer matrix, resulting in inefficient heat dissipation, local overheating, and accelerated degradation during thermal loads. To overcome these limitations, composite materials can be modified with buckypapers—thin, densely interconnected layers of carbon nanotubes (CNTs). In this study, sixteen 8552/IM7 prepreg plies were processed with up to nine buckypapers and strategically placed at various positions. The resulting nanocomposites were evaluated for manufacturability, material properties, and thermal resistance. The findings reveal that prepreg plies provide only limited matrix material for buckypaper infiltration. Nonetheless, up to five buckypapers, corresponding to 8 wt.% CNTs, can be incorporated into the material without inducing matrix depletion defects. This integration significantly enhances the material’s thermal properties while maintaining its mechanical integrity. The nanotubes embedded in the matrix achieve an effective thermal conductivity of up to 7 W/(m·K) based on theoretical modeling. As a result, under one-sided thermal irradiation at 50 kW/m², thermo-induced damage and strength loss can be delayed by up to 20%. Therefore, thermal resistance is primarily determined by the nanotube concentration, whereas the arrangement of the buckypapers affects the material quality. Since this innovative approach enables the targeted integration of high particle fractions, it offers substantial potential for improving the safety and reliability of CFRP under thermal stress. Full article

Orthohantavirus infection is a zoonotic disease transmitted to humans through contact with infected rodents. In Eurasia, Old World Orthohantaviruses can cause haemorrhagic fever with renal syndrome (HFRS), while in the Americas, New World Orthohantaviruses are responsible for hantavirus cardiopulmonary syndrome (HCPS). In Kazakhstan, the first recorded cases of HFRS appeared in the West Kazakhstan region in 2000, which has since then been established as an endemic area due to the presence of stable rodent reservoirs and recurring human infections. Routine diagnosis of HFRS in this region relies primarily on immunoassays. To enhance diagnostic precision, we aimed to implement both serological and molecular methods on samples from suspected HFRS cases in the endemic West Kazakhstan region and non-endemic Almaty City. A total of 139 paired serum, saliva, and urine samples were analysed using IgM/IgG ELISA, immunoblot assays, and qPCR. Our findings confirm that suspected HFRS cases in West Kazakhstan are associated with the Puumala virus serotype. Full article

Drinking Water Safety Plans (DWSP) in buildings serve to identify health hazards associated with the drinking water system. Sanitation Safety Plans (SSP) fulfill the same purpose for the sewage system. Water Safety Plans (WSP) include DWSPs, SSPs, and water systems like gray water and firefighting water. WSPs are based on a high-quality description of the water systems. This paper presents a new methodology for describing water systems. In contrast to previous approaches, the system description begins at the point where the water is consumed. These points of use are described using ecomaps, which are then supplemented with information about the pipe network. This approach makes it possible to fulfill four relevant premises: (1) the system description includes all essential parts of the drinking water installation, (2) the system description is possible with usual equipment, (3) the system description can be carried out with the least possible additional personnel costs, and (4) the system description is controllable, versionable, changeable, and forgery-proof. The ecomaps created in this way are suitable for the next step within the WSP framework, namely hazard and risk assessment. In addition, the ecomaps can be integrated into a quality, occupational safety, or environmental management system. Aspects of water security can be added to enable the ecomaps to be used as the basis for a total integrated water management system. Full article

Background: Headache disorders, particularly migraine, are a leading cause of disability among active-duty military personnel, significantly affecting operational readiness and fitness for duty. Despite their high prevalence, limited data exist on how headache disorders are managed within military primary care systems. This study aimed to evaluate diagnostic confidence, treatment strategies, and structural challenges in the management of headache disorders from the perspective of military primary care physicians. Methods: A prospective, nationwide cross-sectional survey was conducted between May and July 2023 among all active-duty military physicians in primary care roles. An anonymous 15-item questionnaire assessed diagnostic practices, therapeutic approaches, referral pathways, perceived knowledge gaps, and suggestions for system improvements. The survey was distributed across military medical centers and outpatient clinics in Germany. Results: Ninety military physicians participated. Migraine and tension-type headache were commonly encountered, with 70% having treated at least one headache patient in the week prior to the survey. Diagnostic confidence was high for migraine (83.4%) and tension-type headache (77.8%) but lower for medication-overuse headache (65.5%) and cluster headache (47.8%). Acute treatment was widely implemented, but only 27.8% of respondents regularly initiated preventive therapies. Awareness of clinical guidelines was limited: only 23.3% were familiar with the ICHD-3, and just 58.9% with national headache treatment guidelines. Respondents expressed strong demand for targeted education, practical diagnostic tools, and improved interdisciplinary coordination. Conclusions: Headache disorders are a prevalent and clinically significant issue in military primary care. While military physicians show high engagement, important gaps exist in preventive treatment, guideline familiarity, and access to specialist care. Structured training, standardized treatment protocols, and system-level improvements are essential to optimize headache care and maintain operational readiness. Full article

Background/Objectives: Cryptococcosis, caused by Cryptococcus neoformans and Cryptococcus gattii species complexes, remains a significant health concern, particularly among immunocompromised patients. The emergence of antifungal resistance and toxicity of conventional treatment underscore the urgent need for novel therapeutic approaches. Combination therapies represent a promising strategy to enhance efficacy and overcome resistance. This study investigated the antifungal activity of five naphthoquinones against nine isolates of Cryptococcus spp. and assessed their synergistic effects with amphotericin B (AmB). Methods: In this study, five selected naphthoquinones were evaluated for their antifungal activity against Cryptococcus spp. isolates using broth microdilution assays to determine minimum inhibitory concentrations (MICs), according to CLSI guidelines. The potential synergistic effect with AmB was assessed using checkerboard assays, with synergy interpreted based on the fractional inhibitory concentration index (FICI). Cytotoxicity was evaluated in MRC-5 human lung fibroblast cells using the MTT assay. Results: Among the compounds tested, 2-methoxynaphthalene-1,4-dione (2-MNQ) demonstrated antifungal activity, with MIC values ranging from 3.12 to 12.5 µg/mL. Checkerboard assays revealed a synergistic interaction between 2-MNQ and AmB, with a fractional inhibitory concentration index (FICI) of 0.27. The combination reduced the MIC of AmB by 4.17-fold. These findings highlight the potential of synthetic naphthoquinones, particularly 2-MNQ, as effective antifungal agents with synergistic properties when combined with AmB. The observed synergy suggests complementary mechanisms, including increased fungal membrane permeability and oxidative stress induction. Conclusions: This study highlights the potential of 2-MNQ and 2,3-DBNQ as antifungal candidates against Cryptococcus spp., with emphasis on the synergistic interaction observed between 2-MNQ and amphotericin B. The findings reinforce the importance of structural modifications in naphthoquinones to enhance antifungal activity and support the need for further preclinical studies investigating combination therapies aimed at improving treatment efficacy in patients with cryptococcosis. Full article

This paper presents an analysis of the stability margin improvement (SMI), which is also known as stall margin improvement, achieved by continuous tip air injection. New piezoelectric actuators were designed and manufactured with a new engine inlet for the Larzac 04 C5 jet engine. It has noninvasive injection positions that do not have any measurable effect on the inlet air flow when it is switched off. The main focus of the system design was to achieve high power of the injected air and, as a result, a high SMI. The results presented enable a maximum SMI of 99%. A variety of engine operating conditions and injection positions were experimentally tested and discussed regarding SMI. Additionally, the complex relationship between SMI gains and thrust specific fuel consumption (TSFC) is explored in a power balance analysis, revealing a trade-off between SMI improvement and increased energy consumption. Full article

The aim of this study was to develop basic findings for a continuous Signal Quality Monitoring system based on a measurement campaign. Four Galileo satellites were repeatedly recorded, using a dish antenna, and their metrics were analyzed. Due to the stable course, thresholds for the detection of threat models can be determined. These values were tested against simulated signals and the sensitivity of the detection was found to be satisfactory. Based on the convergence behavior of the data, a measurement duration of 180–200 s can be recommended. Finally, the influence of the GPU and memory clock on the performance of predefined conditions close to the receiver was tested. The core clock of the GPU was identified as the bottleneck of the processing. Full article