Occurrence, Prevention and Control of Mycotoxins and Mycotoxigenic Fungi on Nuts and Dried Fruits

A special issue of Toxins (ISSN 2072-6651). This special issue belongs to the section "Mycotoxins".

Deadline for manuscript submissions: closed (31 March 2020) | Viewed by 8469

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Guest Editor
Department of Agricultural, Forest and Food Sciences (DISAFA) and AGROINNOVA (Centre of Competence for the Innovation in the Agro-Environmental Sector), University of Turin, 10124 Turin, Italy
Interests: plant pathology; crop protection; molecular diagnostics; biological control; postharvest; mycotoxins
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Special Issue Information

Dear Colleagues,

Nuts and dried fruits are important sources of nutrients for humans. A large number of nuts, particularly appreciated for their oil content, are used in cooking, eaten raw, sprouted, roasted as a snack food, or pressed for oil. The most important nuts are almond, Brazil nut, cashew, chestnut, macadamia, peanut, pecan, pine nut, pistachio and walnut. The consumption of dried fruits, fresh fruits from which the majority of water content has been removed, retaining the nutritional value, is widespread. Nearly half of the dried fruits consumed are raisins, followed by dates, prunes, figs, apricots, peaches, apples, and pears. Among the main fungal genera present in the microbiota of nuts and dried fruits, there are Fusarium, Aspergillus, Alternaria, and Penicillium, which are able to contaminate nuts and dried fruits with mycotoxins. Aflatoxins and ochratoxins are among the most important mycotoxins occurring on nuts and dried fruits. The most effective tool against mycotoxins is prevention of mold growth in the field and during postharvest. Environmental factors can greatly affect mycotoxin production by fungal species. Predictive models have been developed as decision support systems to plan appropriate crop protection strategies in field. Innovative detection and diagnostic tools are available to monitor the occurrence of mycotoxigenic fungi in field and after harvest. When contamination is not prevented, several approaches can be employed to help to remove mycotoxins from the contaminated commodities, including physical, chemical, and biological techniques. Detoxification processes should destroy or inactivate mycotoxins, by guaranteeing the nutritional value of food. Research is needed to study the fate of mycotoxins during decontamination, detoxification, and food processing. A holistic approach should be adopted to monitor, prevent, and control mycotoxigenic fungi and mycotoxins on nuts and dried fruits.

Prof. Dr. Davide Spadaro
Guest Editor

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Keywords

  • aflatoxins
  • control
  • decontamination
  • detoxification
  • dried fruit
  • monitoring
  • mycotoxin
  • nut
  • occurrence
  • prevention

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Published Papers (2 papers)

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Research

16 pages, 950 KiB  
Article
HPLC-MS/MS Method for the Detection of Selected Toxic Metabolites Produced by Penicillium spp. in Nuts
by Davide Spadaro, Giovanna Roberta Meloni, Ilenia Siciliano, Simona Prencipe and Maria Lodovica Gullino
Toxins 2020, 12(5), 307; https://doi.org/10.3390/toxins12050307 - 8 May 2020
Cited by 10 | Viewed by 4320
Abstract
Penicillium spp. are emerging as producers of mycotoxins and other toxic metabolites in nuts. A HPLC-MS/MS method was developed to detect 19 metabolites produced by Penicillium spp. on chestnuts, hazelnuts, walnuts and almonds. Two extraction methods were developed, one for chestnuts and one [...] Read more.
Penicillium spp. are emerging as producers of mycotoxins and other toxic metabolites in nuts. A HPLC-MS/MS method was developed to detect 19 metabolites produced by Penicillium spp. on chestnuts, hazelnuts, walnuts and almonds. Two extraction methods were developed, one for chestnuts and one for the other three nuts. The recovery, LOD, LOQ and matrix effect were determined for each analyte and matrix. Correlation coefficients were always >99.99%. In walnuts, a strong signal suppression was observed for most analytes and patulin could not be detected. Six strains: Penicillium bialowiezense, P. brevicompactum, P. crustosum, P. expansum, P. glabrum and P. solitum, isolated from chestnuts, were inoculated on four nuts. Chestnuts favored the production of the largest number of Penicillium toxic metabolites. The method was used for the analysis of 41 commercial samples: 71% showed to be contaminated by Penicillium-toxins. Cyclopenin and cyclopenol were the most frequently detected metabolites, with an incidence of 32% and 68%, respectively. Due to the risk of contamination of nuts with Penicillium-toxins, future studies and legislation should consider a larger number of mycotoxins. Full article
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15 pages, 1517 KiB  
Article
Natural Occurrence of Deoxynivalenol and Its Acetylated Derivatives in Chinese Maize and Wheat Collected in 2017
by Pianpian Yan, Zhezhe Liu, Shiqiao Liu, Liyun Yao, Yan Liu, Yongning Wu and Zhiyong Gong
Toxins 2020, 12(3), 200; https://doi.org/10.3390/toxins12030200 - 22 Mar 2020
Cited by 56 | Viewed by 3325
Abstract
Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock. In this study, 579 wheat samples and 606 maize samples intended for consumption were collected from China in 2017 and [...] Read more.
Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock. In this study, 579 wheat samples and 606 maize samples intended for consumption were collected from China in 2017 and analyzed to determine the co-occurrence of type-B trichothecenes (DON, 3-ADON, and 15-ADON). All the wheat samples tested positive for DON, while 99.83% of the maize samples were DON-positive with mean DON concentrations of 165.87 and 175.30 μg/kg, respectively. Per the Chinese standard limits for DON, 3.63% of wheat and 2.97% of the maize samples were above the maximum limit of 1000 μg/kg. The DON derivatives (3-ADON and 15-ADON) were less frequently found and were present at lower levels than DON in wheat. 3-ADON and 15-ADON had incidences of 13.53% and 76.40%, respectively, in maize. By analyzing the distribution ratio of DON and its derivatives in wheat and maize, DON (95.51%) was the predominant toxin detected in wheat samples, followed by 3.97% for the combination of DON + 3-ADON, while DON + 3-ADON + 15-ADON and DON + 15-ADON were only found in 0.17% and 0.35% of wheat samples, respectively. Additionally, a large amount of the maize samples were contaminated with DON + 15-ADON (64.19%) and DON (22.11%). The samples with a combination of DON + 3-ADON and DON + 3-ADON + 15-ADON accounted for 1.32% and 12.21%, respectively. Only one maize sample did not contain all three mycotoxins. Our study shows the necessity of raising awareness of the co-occurrence of mycotoxin contamination in grains from China to protect consumers from the risk of exposure to DON and its derivatives. Full article
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