Foodborne Toxins: Immunodetection and Analysis of Pathogenic Bacterial Toxins

A special issue of Toxins (ISSN 2072-6651). This special issue belongs to the section "Bacterial Toxins".

Deadline for manuscript submissions: 31 December 2024 | Viewed by 501

Special Issue Editors

Foodborne Toxin Detection and Prevention Research Unit, Western Regional Research Center, USDA‐ARS, 800 Buchanan St., Albany, CA 94710, USA
Interests: molecular tools and technologies for rapid; accurate and sensitive detection and quantification of zoonotic pathogens and toxins in food; mechanisms of interactions between bacterial toxins and host cells; binding between antigen and antibody or receptors
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Guest Editor
Foodborne Toxin Detection and Prevention Research Unit, Western Regional Research Center, USDA‐ARS, 800 Buchanan St., Albany, CA 94710, USA
Interests: foodborne toxins; antifungal agents; mechanisms of interactions between bacterial toxins and host cells
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Foodborne bacterial diseases are a major cause of human morbidity and mortality. They are a global concern that affects public health and places, causing undue economic burdens on consumers as well as significant economic losses for farmers, manufacturers, and retailers. Bacterial toxins are the major virulence factors that promote infection. In many cases, the production of toxins and the subsequent intoxication of the host after the oral ingestion of contaminated foods leads to the development of disease. Recent foodborne outbreaks have also provided new insights into newly described bacterial toxins, which are not typically regarded as foodborne pathogens. Current technologies for the detection of bacterial toxins include many different platforms including a multitude of immune-based assays (i.e., ELISAs, biosensors, etc.). Therefore, to ensure a safe food supply, advances in diagnostic detection technologies that are rapid, sensitive, and portable are critical. New immune-based methods to detect toxins from known and unknown bacterial pathogens in a variety of complex matrices must be developed to aid food processors and regulatory agencies to adequately monitor the safety of our food. This Special Issue of “Foodborne Toxins: Immunodetection and Analysis of Pathogenic Bacterial Toxins” will focus on the analysis of bacterial toxins and advances in immune-based detection approaches. Review and research papers describing established and novel concepts are welcome.

Dr. Xiaohua He
Dr. Christina Tam
Guest Editors

Manuscript Submission Information

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Keywords

  • bacterial toxins
  • food safety
  • immunoassays
  • detection
  • ELISA
  • biosensors
  • antibodies

Published Papers (1 paper)

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Research

14 pages, 1534 KiB  
Article
Rapid and Sensitive Detection of Shiga Toxin-Producing Escherichia coli (STEC) from Food Matrices Using the CANARY Biosensor Assay
by Christina C. Tam, Wen-Xian Du, Yangyang Wang, Andrew R. Flannery and Xiaohua He
Toxins 2024, 16(7), 325; https://doi.org/10.3390/toxins16070325 - 22 Jul 2024
Viewed by 281
Abstract
Shiga toxin-producing Escherichia coli (STEC) causes a wide spectrum of diseases including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Previously, we developed a rapid, sensitive, and potentially portable assay that identified STEC by detecting Shiga toxin (Stx) using a B-cell based biosensor platform. [...] Read more.
Shiga toxin-producing Escherichia coli (STEC) causes a wide spectrum of diseases including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Previously, we developed a rapid, sensitive, and potentially portable assay that identified STEC by detecting Shiga toxin (Stx) using a B-cell based biosensor platform. We applied this assay to detect Stx2 present in food samples that have been implicated in previous STEC foodborne outbreaks (milk, lettuce, and beef). The STEC enrichment medium, modified Tryptone Soy Broth (mTSB), inhibited the biosensor assay, but dilution with the assay buffer relieved this effect. Results with Stx2a toxoid-spiked food samples indicated an estimated limit of detection (LOD) of ≈4 ng/mL. When this assay was applied to food samples inoculated with STEC, it was able to detect 0.4 CFU/g or 0.4 CFU/mL of STEC at 16 h post incubation (hpi) in an enrichment medium containing mitomycin C. Importantly, this assay was even able to detect STEC strains that were high expressors of Stx2 at 8 hpi. These results indicate that the STEC CANARY biosensor assay is a rapid and sensitive assay applicable for detection of STEC contamination in food with minimal sample processing that can complement the current Food Safety Inspection Service (US) methodologies for STEC. Full article
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