Nucleotide Metabolism in Plants

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Physiology and Metabolism".

Deadline for manuscript submissions: closed (30 June 2020) | Viewed by 5950

Special Issue Editors


E-Mail Website
Guest Editor
Departamento de Botánica, Ecología y Fisiología Vegetal, Instituto Andaluz de Biotecnología, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain
Interests: nucleotides; ureides; ribonucleases; senescence; germination
Special Issues, Collections and Topics in MDPI journals

E-Mail
Guest Editor
Departamento de Botánica, Ecología y Fisiología Vegetal, Instituto Andaluz de Biotecnología, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain
Interests: plant nitrogen metabolism; nucleotide metabolism; ureides; high light acclimation; tocopherols
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nucleotides are crucial compounds for plant metabolism and development, as they are components of nucleic acids, vitamins, and coenzymes and are important as energy donors, and extracellular ATP acts as a signaling molecule. In animals, nucleotide metabolism has been widely studied due to its medical applications. Nevertheless, much less is known about its metabolism in plants.

Nucleotide metabolism can be divided into three parts: de novo synthesis, salvage of nucleosides and nucleobases, and catabolism of purines and pyrimidines. In plant tissues, nucleic acid could be a source for the salvage pathway, since nucleic acids are relatively abundant molecules. Purine metabolism has an additional role as a precursor of ureides, compounds involved in nitrogen transport in some legumes. In addition, a role in the response to abiotic stresses has been associated to purine degradation metabolites in plants. In addition, nucleotide, nucleoside, and nucleobases transport along subcellular compartments and between organs is an essential process in all living organisms. Despite its important contribution to plant metabolism, many aspects of nucleotide metabolism in plants are still poorly understood, perhaps because of the complexity of the multiple biochemical reactions involved in all nucleotide metabolism.

This Special Issue will focus on all these aspects of nucleotide metabolism, including de novo synthesis, salvage, catabolism, and transport. Original research papers, reviews, and perspectives are welcome.

Dr. Pedro Piedras
Dr. Gregorio Gálvez-Valdivieso
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nucleotides, nucleosides, and nucleobases
  • De novo synthesis of purine or pyrimidines
  • nucleotide salvage pathways
  • purine or pyrimidine catabolism
  • nucleotides as signaling molecules
  • nucleotide, nucleoside or nucleobase transport

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

14 pages, 1412 KiB  
Article
Maize Thymidine Kinase Activity Is Present throughout Plant Development and Its Heterologous Expression Confers Tolerance to an Organellar DNA-Damaging Agent
by Manuela Nájera-Martínez, José Antonio Pedroza-García, Luis Jiro Suzuri-Hernández, Christelle Mazubert, Jeannine Drouin-Wahbi, Jorge Vázquez-Ramos, Cécile Raynaud and Javier Plasencia
Plants 2020, 9(8), 930; https://doi.org/10.3390/plants9080930 - 23 Jul 2020
Cited by 1 | Viewed by 2320
Abstract
Thymidine kinase 1 (TK1) phosphorylates thymidine nucleosides to generate thymidine monophosphate. This reaction belongs to the pyrimidine salvage route that is phylogenetically conserved. In the model plant Arabidopsis thaliana, TK activity contributes to maintain nuclear and organellar genome integrity by providing deoxythymidine-triphosphate [...] Read more.
Thymidine kinase 1 (TK1) phosphorylates thymidine nucleosides to generate thymidine monophosphate. This reaction belongs to the pyrimidine salvage route that is phylogenetically conserved. In the model plant Arabidopsis thaliana, TK activity contributes to maintain nuclear and organellar genome integrity by providing deoxythymidine-triphosphate (dTTP) for DNA synthesis. Arabidopsis has two TK1 genes (TK1a and TK1b) and double mutants show an albino phenotype and develop poorly. In contrast, maize (Zea mays L.) has a single TK1 (ZmTK1) gene and mutant plants are albino and display reduced genome copy number in chloroplasts. We studied the role of ZmTK1 during development and genotoxic stress response by assessing its activity at different developmental stages and by complementing Arabidopsis tk1 mutants. We found that ZmTK1 transcripts and activity are present during germination and throughout maize development. We show that ZmTK1 translocation to chloroplasts depends on a 72-amino-acid N-signal and its plastid localization is consistent with its ability to complement Arabidopsis tk1b mutants which are hypersensitive to ciprofloxacin (CIP), a genotoxic agent to organellar DNA. Also, ZmTK1 partly complemented the Arabidopsis double mutant plants during development. Our results contribute to the understanding of TK1 function in monocot species as an organellar enzyme for genome replication and repair. Full article
(This article belongs to the Special Issue Nucleotide Metabolism in Plants)
Show Figures

Graphical abstract

13 pages, 3631 KiB  
Article
Biochemical and Molecular Characterization of PvNTD2, a Nucleotidase Highly Expressed in Nodules from Phaseolus vulgaris
by Gregorio Galvez-Valdivieso, Elena Delgado-Garcia, Mercedes Diaz-Baena, Oscar Montaño, Francisco A. Quiles, Manuel Pineda and Pedro Piedras
Plants 2020, 9(2), 171; https://doi.org/10.3390/plants9020171 - 1 Feb 2020
Cited by 3 | Viewed by 2058
Abstract
Nucleotides are molecules of great importance in plant physiology. In addition to being elementary units of the genetic material, nucleotides are involved in bio-energetic processes, play a role as cofactors, and are also components of secondary metabolites and the hormone cytokinin. The common [...] Read more.
Nucleotides are molecules of great importance in plant physiology. In addition to being elementary units of the genetic material, nucleotides are involved in bio-energetic processes, play a role as cofactors, and are also components of secondary metabolites and the hormone cytokinin. The common bean (Phaseolus vulgaris) is a legume that transports the nitrogen fixed in nodules as ureides, compounds synthetized from purine nucleotides. The first step in this pathway is the removal of the 5’-phosphate group by a phosphatase. In this study, a gene that codes for a putative nucleotidase (PvNTD2) has been identified in P. vulgaris. The predicted peptide contains the conserved domains for haloacid dehalogenase-like hydrolase superfamily. The protein has been overexpressed in Escherichia coli, and the purified protein showed molybdate-resistant phosphatase activity with nucleoside monophosphates as substrates, confirming that the identified gene codes for a nucleotidase. The optimum pH for the activity was 7–7.5. The recombinant enzyme did not show special affinity for any particular nucleotide, although the behaviour with AMP was different from that with the other nucleotides. The activity was inhibited by adenosine, and a regulatory role for this nucleoside was proposed. The expression pattern of PvNTD2 shows that it is ubiquitously expressed in all the tissues analysed, with higher expression in nodules of adult plants. The expression was maintained during leaf ontogeny, and it was induced during seedling development. Unlike PvNTD1, another NTD previously described in common bean, the high expression of PvNTD2 was maintained during nodule development, and its possible role in this organ is discussed. Full article
(This article belongs to the Special Issue Nucleotide Metabolism in Plants)
Show Figures

Figure 1

Back to TopTop