Biotechnology of Plant Tissue Culture

A special issue of Plants (ISSN 2223-7747).

Deadline for manuscript submissions: closed (20 October 2022) | Viewed by 4995

Special Issue Editors


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Guest Editor
Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
Interests: plant tissue culture; plant molecular biology

E-Mail Website
Guest Editor
Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
Interests: plant secondary metabolites; environmental adaptation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Alsophila spinulosa (Wall. ex Hook) Tryon is one of the few remaining tree-shaped ferns on Earth. It is a "living fossil" of Mesozoic ferns and is of great significance for the study of paleoclimate and paleo-environmental changes. It has attracted much attention in the field of science for its important scientific research, ornamental, and medicinal value. In the present study, we first report on the tissue culture and regeneration of A. spinulosa. Furthermore, a complete germination of the spore on an MS medium to the rooting sporophyte’s transplantation is established. Simultaneously, the different factors affecting the formation process of the sporophytes are investigated. In addition, we directly induced sporophytes from the gametophytes, as well as callus induction and callus differentiation into the sporophytes. Moreover, we directly induced new sporophytes from the young sporophytes, which improved its proliferation and regeneration system. This Special Issue mainly introduces the in vitro propagation, tissue culture and regeneration ability, and the process of somatic embryogenesis.

Prof. Dr. Guohua Ma
Dr. Xinhua Zhang
Guest Editors

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Keywords

  • Alsophila spinulosa
  • spore germination
  • gametophytes
  • sporophytes
  • regeneration
  • transplantation

Published Papers (2 papers)

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Research

11 pages, 2886 KiB  
Article
Optimization of Regeneration and Agrobacterium-Mediated Transformation Protocols for Bi and Multilocular Varieties of Brassica rapa
by Uzair Muhammad Khan, Nabeel Shaheen, Ayesha Farooq, Rizwana Maqbool, Sultan Habibullah Khan, Muhammad Tehseen Azhar, Iqrar Ahmad Rana and Hyojin Seo
Plants 2023, 12(1), 161; https://doi.org/10.3390/plants12010161 - 29 Dec 2022
Cited by 3 | Viewed by 1803
Abstract
The regeneration of the high-yielding multilocular types has not been attempted, although successful regeneration and transformation in brassica have been done. Here, we report efficient regeneration and transformation protocols for two B. rapa genotypes; UAF11 and Toria. The B. rapa cv UAF11 is [...] Read more.
The regeneration of the high-yielding multilocular types has not been attempted, although successful regeneration and transformation in brassica have been done. Here, we report efficient regeneration and transformation protocols for two B. rapa genotypes; UAF11 and Toria. The B. rapa cv UAF11 is a multilocular, non-shattering, and high-yielding genotype, while Toria is the bilocular type. For UAF11 8 shoots and for Toria 7 shoots, explants were observed on MS supplemented with 3 mg/L BAP + 0.4 mg/L NAA + 0.01 mg/L GA3 + 5 mg/L AgNO3 + 0.75 mg/L Potassium Iodide (KI), MS salt supplemented with 1 mg/L IBA and 0.37 mg/L KI produced an equal number of roots (3) in UAF11 and Toria. For the establishment of transformation protocols, Agrobacterium-mediated floral dip transformation was attempted using different induction media, infection time, and flower stages. The induction medium III yielded a maximum of 7.2% transformants on half-opened flowers and 5.2% transformants on fully opened flowers in UAF11 and Toria, respectively, with 15 min of inoculation. This study would provide the basis for the improvement of tissue culture and transformation protocols in multilocular and bilocular Brassica genotypes. Full article
(This article belongs to the Special Issue Biotechnology of Plant Tissue Culture)
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16 pages, 1690 KiB  
Article
Antioxidants Application Enhances Regeneration and Conversion of Date Palm (Phoenix dactylifera L.) Somatic Embryos
by Amal F. M. Zein El Din, Rasmia S. S. Darwesh, Mohamed F. M. Ibrahim, Gehan M. Y. Salama, Ibrahim M. Shams El-Din, Walid B. Abdelaal, Ghada A. Ali, Maha S. Elsayed, Ismail A. Ismail, Eldessoky S. Dessoky and Yasmin M. R. Abdellatif
Plants 2022, 11(15), 2023; https://doi.org/10.3390/plants11152023 - 03 Aug 2022
Cited by 2 | Viewed by 1786
Abstract
Many embryogenic systems have been designed to generate somatic embryos (SEs) with the morphology, biochemistry, and vigor uniformity of zygotic embryos (ZEs). During the current investigation, several antioxidants were added to the maturation media of the developing somatic embryos of date palm. Explant [...] Read more.
Many embryogenic systems have been designed to generate somatic embryos (SEs) with the morphology, biochemistry, and vigor uniformity of zygotic embryos (ZEs). During the current investigation, several antioxidants were added to the maturation media of the developing somatic embryos of date palm. Explant material was a friable embryogenic callus that was placed in maturation media containing ABA at 0.5 mg L−1, 5 g L−1 polyethylene glycol, and 10 g L−1 phytagel. Furthermore, α-tocopherol or reduced glutathione (GSH) were used separately at (25 and 50 mg L−1). These treatments were compared to a widely used date palm combination of reduced ascorbic acid (ASC) and citric acid at 150 and 100 mg L−1, respectively, and to the medium free from any antioxidants. The relative growth percentage of embryogenic callus (EC), globularization degree, differentiation%, and SEs number were significantly increased with GSH (50 mg L−1). Additionally, the latter treatment significantly enhanced the conversion% of SEs and the number of secondary somatic embryos (SSEs). ASC and citric acid treatment increased leaf length, while α-tochopherol (50 mg L−1) elevated the number of leaves plantlet−1. GSH at 50 mg L−1 catalyzed the activities of polyphenol oxidase (PPO) and peroxidase (POD) in EC and enhanced the accumulation of proteins in SEs. Full article
(This article belongs to the Special Issue Biotechnology of Plant Tissue Culture)
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