Special Issue "Advances in Grapevine Genotyping, Varietal Identification and Germplasm Collections"

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: 20 November 2022 | Viewed by 4060

Special Issue Editors

Dr. Giacomo Morreale
E-Mail Website
Guest Editor
Consiglio per la Ricerca in agricoltura e l’analisi dell’economia Agraria (CREA), Rome, Italy
Dr. Carlo Bergamini
E-Mail Website
Guest Editor
Council for Agricultural Research and Economics, Research Unit for Viticulture and Enology in Southern Italy (CRA-UTV), Turi, Italy
Dr. Alessandra Zombardo
E-Mail Website
Guest Editor
Council for Agricultural Research and Economics, CREA - Research centre for Viticulture and Enology, Viale S. Margherita, 80, 52100 Arezzo, Italy
Dr. Luca Nerva
E-Mail Website
Guest Editor
Council for Agricultural Research and Economics, CREA - Research Centre for Viticulture and Enology, Viale XXVIII Aprile 26, 31015 Conegliano (Treviso), Italy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Great morphological and genetic variability characterizes both the genus Vitis and Vitis vinifera L. subsp. Sativa alone. The number of cultivars remains an estimate. Whereas, there are germplasm collections scattered around the world where it is possible to identify thousands of cultivar and ask for samples.

The above collections can be used for various studies: pedigree studies, gene mutants, breeding, genome editing and so on and so forth.

In order to study the importance of a gene or a pool of genes the use of the collections could be of a primary importance because it might allow a first inspection of the  effects on the phenotype.

This Special Issue is focused on the genetics and genomics variability of the Vitis germplasm and the use of collections to understand the current status of genetic diversity analysis, the situation of germplasm collections and advancements in researches that connect genes to phenotype and those to be involved in biotic and abiotic stresses.

The submission of reviews, research articles, and short communications focused on the above topics is welcome.

Dr. Giacomo Morreale
Dr. Carlo Bergamini
Dr. Alessandra Zombardo
Dr. Luca Nerva
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Vitis spp
  • grapevine genetics
  • cultivars
  • germplasm collections
  • relation of genes to phenotypes
  • biotic and abiotic stresses

Published Papers (3 papers)

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Research

Article
Study of Inter- and Intra-varietal Genetic Variability in Grapevine Cultivars
Plants 2022, 11(3), 397; https://doi.org/10.3390/plants11030397 - 31 Jan 2022
Viewed by 747
Abstract
Vitis vinifera includes a large number of cultivars that are further distinguished in biotypes and clones, and it is actually hard to differentiate them, even through complex molecular techniques. In this work, the plant materials of 56 putative Sangiovese and 14 putative Montepulciano [...] Read more.
Vitis vinifera includes a large number of cultivars that are further distinguished in biotypes and clones, and it is actually hard to differentiate them, even through complex molecular techniques. In this work, the plant materials of 56 putative Sangiovese and 14 putative Montepulciano biotypes, two of the most widespread black-berried Italian cultivars, were collected in different wine-growing areas of Italy distributed in 13 regions, from north to south. Firstly, the samples were analyzed using SSR markers to have proper varietal identification. According to the results, the genotypes belonged to three different cultivars: Sangiovese, Sanforte, and Montepulciano. Subsequently, the samples were investigated using AFLP, SAMPL, M-AFLP, and I-SSR molecular markers to estimate their intra-varietal genetic variability. The DNA marker-based method used turned out to be performing to bring out the geographic differences among the biotypes screened, and it can therefore be considered as a powerful tool available for all the grapevine varieties. Full article
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Article
SNP-Based Analysis Reveals Authenticity and Genetic Similarity of Russian Indigenous V. vinifera Grape Cultivars
Plants 2021, 10(12), 2696; https://doi.org/10.3390/plants10122696 - 08 Dec 2021
Viewed by 1715
Abstract
9 Russian Vitis vinifera grape varieties and the European variety Muscat Hamburg were sequenced and genotyped using 527 SNPs (single nucleotide polymorphisms) with high minor allele frequency for the first time. The data were coupled with previously identified genotypes of 783 varieties and [...] Read more.
9 Russian Vitis vinifera grape varieties and the European variety Muscat Hamburg were sequenced and genotyped using 527 SNPs (single nucleotide polymorphisms) with high minor allele frequency for the first time. The data were coupled with previously identified genotypes of 783 varieties and subjected to parentage and population analysis. As a result, contrary to the historical and ampelographic data published in many sources from 1800 to 2012, only two of the nine Russian varieties (Pukhlyakovskiy Belyi and Sibirkovyi) were related to foreign ones and were obviously imported from Europe to the Russian Empire. The remaining seven varieties, led by Krasnostop Zolotovskiy, are not directly related either in the Caucasus or in Europe, they form separate clusters on the genetic distance-based dendrogram and the world parentage network of V. vinifera. The resulting pedigree of Muscat Hamburg and its descendants is in accordance with SSR-based (simple sequence repeats) studies and the described pedigree of this variety which confirms the use of the reduced SNP set for further studies. Full article
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Article
Recovery, Molecular Characterization, and Ampelographic Assessment of Marginal Grapevine Germplasm from Southern Umbria (Central Italy)
Plants 2021, 10(8), 1539; https://doi.org/10.3390/plants10081539 - 27 Jul 2021
Cited by 1 | Viewed by 811
Abstract
The protection of grapevine biodiversity and the safeguarding of genetic variability are certainly primary and topical objectives for wine research, especially in territories historically devoted to viticulture. To assess the autochthonous germplasm of three different districts of Southern Umbria (Central Italy), the plant [...] Read more.
The protection of grapevine biodiversity and the safeguarding of genetic variability are certainly primary and topical objectives for wine research, especially in territories historically devoted to viticulture. To assess the autochthonous germplasm of three different districts of Southern Umbria (Central Italy), the plant material of 70 grapevines retrieved from reforested land plots or old vineyards was collected, and their genetic identity was investigated using 13 microsatellite markers (SSR). The results revealed the presence of 39 unique genotypes, divided into 24 already-known cultivars and 15 never-reported SSR profiles. Most of the grapevine accessions were then vegetatively propagated and cultivated in a vineyard collection both to be protected from extinction and to be evaluated at the ampelographic level. Overall, this work emphasizes the need for recovering the threatened genetic variability that characterizes minor neglected grapevine cultivars or biotypes of Southern Umbria germplasm, and the requirement to revalue and exploit the more valuable genetic resources to enhance the local agri-food economy. Full article
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Recovery, Molecular Characterization, and Ampelographic Assessment of Marginal Grapevine Germplasm from Southern Umbria (Central Italy)
Authors: Alessandra Zombardo
Affiliation: Council for Agricultural Research and Economics, CREA - Research centre for Viticulture and Enology, Viale S. Margherita, 80, 52100 Arezzo, Italy
Abstract: The protection of grapevine biodiversity and the safeguarding of genetic variability are certainly primary and very topical objectives for wine research, especially in territories historically devoted to viticulture. To assess the autochthonous germplasm of three different districts of Southern Umbria (Central Italy), the plant material of 70 grapevines retrieved in reforested land plots or old vineyards was collected and their genetic identity was investigated using 13 microsatellite markers (SSR). The results revealed the presence of 39 unique genotypes, divided into 25 already known cultivars, and 14 never reported SSR profiles. Most of the grapevine accessions were then vegetatively propagated and cultivated in a vineyard collection both to be saved from extinction and to be evaluated at the ampelographic level. Overall, this work emphasizes the need for recovering the threatened genetic variability that characterizes minor neglected grapevine cultivars or biotypes of Southern Umbria germplasm and the requirement to revalue and exploit the more valuable genetic resources to enhance the local agri-food economy.

Title: Study of Inter- and Intra-varietal Genetic Variability in Sangiovese, Sanforte and Montepulciano Grape Cultivars
Authors: Alessandra Zombardo
Affiliation: Council for Agricultural Research and Economics, CREA - Research centre for Viticulture and Enology, Viale S. Margherita, 80, 52100 Arezzo, Italy
Abstract: Vitis vinifera is one of the oldest known domesticated fruit-producing crops, which includes a wide number of cultivated varieties further distinguished in biotypes and clones, that are characterized by great morphological and genetic variability. In the past, several objective taxonomic methods have been developed for grapevine identification, but all those approaches have limitations and there are often classification mistakes. At the present, Simple Sequence Repeats (SSR) are the internationally accepted molecular markers for grapevine identification, but even this method does not allow intra-varietal discriminations. In this work, 56 accessions of putative Sangiovese and 14 of putative Montepulciano from different Italian areas were analyzed to have a correct cultivar identification first, and then to study the differences at the intra-varietal level. Two varieties were identified among the first group: 45 accessions turned out to be Sangiovese, while 11 were Sanforte, two traditionally co-existing cultivars in the Sangiovese D.O.C. or D.O.C.G. areas. All Montepulciano accessions were confirmed as true-to-type. The accessions were then investigated using AFLP, SAMPL, M-AFLP, and ISSR molecular markers that correctly distinguished in separated groups Sangiovese, Sanforte, and Montepulciano. Intra-varietal results have highlighted the existence of genetic variability correlated to the different geographic cultivation areas, for each cultivar screened.

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