Applications and Impacts of Genome Editing

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (31 May 2022) | Viewed by 2906

Special Issue Editors


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Guest Editor
Institute of Field and Vegetable Crops, Novi Sad 21000, Serbia
Interests: biotechnology; biosafety; molecular biology; abiotic and biotic stress resistance; breeding; oil crops
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Guest Editor
Sweet Environmental Consultants, Cambridge CB24 5JA, UK
Interests: crop protection; crop improvement; biotechnology; environmental risk assessment; sustainable agriculture and land use
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Guest Editor
Perseus BV, B-9830 Sint-Martens-Latem, Belgium
Interests: biotechnology; biosafety; GMO; environmental risk assessment; regulation; synthetic biology

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Guest Editor
Institute for Biosafety in Plant Biotechnology, Julius Kuehn Institut, 06484 Quedlinburg, Germany
Interests: genome editing; biotechnology; biosafety; GMO; regulation; meiosis; recombination; synthetic biology
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Special Issue Information

Dear Colleagues,

The main challenges of modern agriculture are maintaining and raising productivity and adapting to climate change while reducing its negative impact on the environment. Genome editing (GE) provides tools to solve these challenges. Although the first GE products are now available, the current regulatory framework and uncertainty about possible applications could affect implementation of GE for plant improvement, along with the free movement of goods, especially in the EU.

The aim of this Special Issue is to provide an overview of the current and future GE technology impacts on plant breeding and agriculture, with a focus on the range of techniques being adopted by breeders, innovative applications, impacts they have on the breeding process and new variety types, as well as agricultural value chains. Since there is a range of factors affecting the acceptance of genome editing technology and its impact on innovation (e.g., ethical issues, communication strategies, and legislation related to or affecting GE and its impact on innovation), this Special Issue will also address the regulatory, socio-economic, and ethical aspects, biosafety research, as well as efficient communication strategies. Original research papers, perspectives, hypotheses, opinions, reviews, modeling approaches, and methods are welcome.

Dr. Dragana Miladinović
Dr. Jeremy Sweet
Dr. Patrick Rüdelsheim
Dr. Thorben Sprink
Guest Editors

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Keywords

  • genome editing
  • crop improvement
  • application
  • climate change
  • impacts
  • sustainable agriculture
  • agricultural footprint

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Published Papers (1 paper)

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Research

24 pages, 2855 KiB  
Article
DNA Free CRISPR/DCAS9 Based Transcriptional Activation System for UGT76G1 Gene in Stevia rebaudiana Bertoni Protoplasts
by Asish Kumar Ghose, Siti Nor Akmar Abdullah, Muhammad Asyraf Md Hatta and Puteri Edaroyati Megat Wahab
Plants 2022, 11(18), 2393; https://doi.org/10.3390/plants11182393 - 14 Sep 2022
Cited by 4 | Viewed by 2360
Abstract
The UDP-glycosyltransferase 76G1 (UGT76G1) is responsible for the conversion of stevioside to rebaudioside A. Four single guide RNAs (sgRNAs) were designed from the UGT76G1 proximal promoter region of stevia by using the online-based tool, benchling. The dCas9 fused with VP64 as [...] Read more.
The UDP-glycosyltransferase 76G1 (UGT76G1) is responsible for the conversion of stevioside to rebaudioside A. Four single guide RNAs (sgRNAs) were designed from the UGT76G1 proximal promoter region of stevia by using the online-based tool, benchling. The dCas9 fused with VP64 as a transcriptional activation domain (TAD) was produced and purified for the formation of ribonucleoproteins (RNPs) by mixing with the in vitro transcribed sgRNAs. Protoplast yield was the highest from leaf mesophyll of in vitro grown stevia plantlets (3.16 × 106/g of FW) using ES5 (1.25% cellulase R-10 and 0.75% macerozyme R-10). The RNPs were delivered into the isolated protoplasts through the Polyethylene glycol (PEG)-mediated transfection method. The highest endogenous activation of the UGT76G1 gene was detected at 27.51-fold after 24 h of transfection with RNP30 consisting of CRISPR/dCas9-TAD with sgRNA30 and a similar activation level was obtained using RNP18, RNP33, and RNP34, produced using sgRNA18, sgRNA33, and sgRNA34, respectively. Activation of UGT76G1 by RNP18 led to a significant increase in the expression of the rate-limiting enzyme UGT85C2 by 2.37-fold and there was an increasing trend in the expression of UGT85C2 using RNP30, RNP33, and RNP34. Successful application of CRISPR/dCas9-TAD RNP in activating specific genes can avoid the negative integration effects of introduced DNA in the host genome. Full article
(This article belongs to the Special Issue Applications and Impacts of Genome Editing)
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