The Role of Tissue Culture in Enhancing Plant Stress Tolerance and Propagation

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Cell Biology".

Deadline for manuscript submissions: 25 August 2025 | Viewed by 1057

Special Issue Editor


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Guest Editor
Agro-Biotechnology Research Unit, Regional Center of Agricultural Research of Marrakech, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principale, Rabat 10090, Morocco
Interests: Plant tissue culture

Special Issue Information

Dear Colleagues,

In recent years, climate change has received a large amount of attention from scientists and policymakers because of its impact on agricultural production and food security. Among the consequences of climate change is that biotic and abiotic pressures are becoming more severe, endangering the sustainability of world agriculture. While the spread of weeds, pests, and phytopathogenic microorganisms to new regions and/or host plants is an example of biotic stresses that are observed as a result of climate change, we have also seen significant increases in the occurrence of abiotic stresses such as salinity, drought, heat, cold, nutritional, and heavy metal stress.

The use of in vitro propagation and regeneration systems represents an efficient approach to improving crop yield and quality. Tissue culture systems are known for their effectiveness in rapidly propagating species and cultivars resistant to abiotic and biotic stresses, improving stress tolerance through genetic transformation, in vitro selection, genetic editing and molecular cloning, and as a tool to understand the mechanisms underlying stress tolerance at the biochemical, physiological, and molecular levels.

This Special Issue, entitled “The Role of Tissue Culture in Enhancing Plant Stress Tolerance and Propagation”, aims to compile up-to-date research and reviews on the use of in vitro culture systems to produce stress-tolerant plants, and to advance our understanding of the various mechanisms underlying enhanced resilience to environmental stress.

Dr. Mouaad Amine Mazri
Guest Editor

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Keywords

  • biotechnology
  • in vitro stress induction
  • micropropagation
  • regeneration
  • stress tolerance

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Published Papers (1 paper)

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Research

11 pages, 3223 KiB  
Article
Effects of Elicitation on Abeliophyllum distichum Leaf Callus and Changes in Verbascoside Content
by Daeho Choi, Yong-Woo Park, Jungmok Kang, Eun-Suk Jung and Hwayong Lee
Plants 2025, 14(9), 1386; https://doi.org/10.3390/plants14091386 - 4 May 2025
Viewed by 265
Abstract
Abeliophyllum distichum is a monotypic species in the family Oleaceae that contains a range of phenolic compounds and components such as coumaric acid, catechin, and verbascoside, the latter of which is a major candidate of commercial interest. In this study, we assessed the [...] Read more.
Abeliophyllum distichum is a monotypic species in the family Oleaceae that contains a range of phenolic compounds and components such as coumaric acid, catechin, and verbascoside, the latter of which is a major candidate of commercial interest. In this study, we assessed the potential for producing verbascoside using callus culture. To enhance callus productivity in this regard, we evaluated the efficacy of treatment with the elicitors salicylic acid (SA) and methyl jasmonate (MeJA) based on changes in verbascoside content with callus development using Petri dish cultures. Whereas the initial content of verbascoside in A. distichum callus was approximately 50 mg/g, in response to treatment with 50 μM MeJA, we detected an increase to approximately 97.05 mg/g. In contrast, treatment with SA had no significant effects on verbascoside content. In addition, we found that the fresh weight of callus receiving elicitor treatment was lower than that of control callus. Conversely, however, in bioreactor cultures, the fresh weight of callus following treatment with 50μM MeJA for 1 week was higher than that of control callus, and the content of verbascoside in callus treated with 50 μM MeJA was higher than that in control callus. Our findings in this study thus indicate that with appropriate elicitation, the production of verbascoside by A. distichum callus pieces can be enhanced. Full article
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