Special Issue "Microfluidic for High-Throughput Screening"

A special issue of Micromachines (ISSN 2072-666X).

Deadline for manuscript submissions: 25 February 2020.

Special Issue Editor

Dr. Michele Zagnoni
E-Mail Website
Guest Editor
University of Strathclyde, Glasgow G1 1XW, UK
Tel. +44(0)141-548-5985
Interests: microfluidics; organ-on-a-chip; in vitro models; drug screening

Special Issue Information

Dear Colleagues,

Microfluidics and lab-on-a-chip technologies have increasingly been used in the past two decades for miniaturizing biochemical analysis and cell-based screening assays. Following the rapid development of a variety of single-phase and multi-phase microfluidic techniques, scalable and complex architectures have been proposed for both single-cell analysis and in vitro models of disease. Therefore, by combining the miniaturization benefits of microfluidics with advances in microfabrication techniques, these systems provide an excellent platform for high-throughput screening applications, offering cost-saving opportunities compared to a standard well-plate based system. Such features offer unique capabilities for screening limited samples of human tissue, ultra-low sample volumes, stem cell research, and for innovating drug discovery, all of which can impact biological investigation, clinical practice, and industrial procedures. In particular, the integration of physiologically relevant in vitro models of disease has great potential to reduce animal-based research and aid precision medicine.

Accordingly, this Special Issue seeks to showcase research papers, short communications, and review articles that focus on novel microfluidic systems and novel methodological application of microfluidic techniques for high-throughput and high content screening, high-throughput experimentation, automation, and large-scale identification/manipulation of biological tissue.

Dr. Michele Zagnoni
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Microfluidics
  • High-throughput screening
  • Large-scale experimentation
  • High content screens
  • Miniaturized drug discovery

Published Papers (2 papers)

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Research

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Open AccessArticle
A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor
Micromachines 2019, 10(10), 685; https://doi.org/10.3390/mi10100685 - 11 Oct 2019
Abstract
A self-activated micropump which is capable of stable velocity transport for a liquid to flow a given distance inside a 3D microchannel has been a dream of microfluidic scientists for a long time. A new self-activated pumping mechanism has been proposed in this [...] Read more.
A self-activated micropump which is capable of stable velocity transport for a liquid to flow a given distance inside a 3D microchannel has been a dream of microfluidic scientists for a long time. A new self-activated pumping mechanism has been proposed in this paper. It is different from the authors’ previous research which relied on the fluid resistance of a quartz capillary tube or end-blocked gas-permeable silicone or a polydimethylsiloxane (PDMS) wall to automate the flow. In this research, an end-open stretched Teflon tube was utilized for passive transport for the first time. A new fluid transmission mode was adopted with the assistance of a cheaper easily accessible oil mixture to achieve stable continuous flow. Finally, this novel micropump has been applied to real-time continuous-flow polymerase chain reactions (PCRs), with an amplification efficiency similar to that of a commercial PCR cycler instrument. Full article
(This article belongs to the Special Issue Microfluidic for High-Throughput Screening)
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Review

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Open AccessReview
Microfluidic Technologies for cfDNA Isolation and Analysis
Micromachines 2019, 10(10), 672; https://doi.org/10.3390/mi10100672 - 03 Oct 2019
Abstract
Cell-free DNA (cfDNA), which promotes precision oncology, has received extensive concern because of its abilities to inform genomic mutations, tumor burden and drug resistance. The absolute quantification of cfDNA concentration has been proved as an independent prognostic biomarker of overall survival. However, the [...] Read more.
Cell-free DNA (cfDNA), which promotes precision oncology, has received extensive concern because of its abilities to inform genomic mutations, tumor burden and drug resistance. The absolute quantification of cfDNA concentration has been proved as an independent prognostic biomarker of overall survival. However, the properties of low abundance and high fragmentation hinder the isolation and further analysis of cfDNA. Microfluidic technologies and lab-on-a-chip (LOC) devices provide an opportunity to deal with cfDNA sample at a micrometer scale, which reduces required sample volume and makes rapid isolation possible. Microfluidic platform also allow for high degree of automation and high-throughput screening without liquid transfer, where rapid and precise examination and quantification could be performed at the same time. Microfluidic technologies applied in cfDNA isolation and analysis are limited and remains to be further explored. This paper reviewed the existing and potential applications of microfluidic technologies in collection and enrichment of cfDNA, quantification, mutation detection and sequencing library construction, followed by discussion of future perspectives. Full article
(This article belongs to the Special Issue Microfluidic for High-Throughput Screening)
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