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Open AccessFeature PaperArticle

Improving Single-Cell Encapsulation Efficiency and Reliability through Neutral Buoyancy of Suspension

by Hangrui Liu 1, Ming Li 2,*, Yan Wang 1, Jim Piper 1,* and Lianmei Jiang 3,*
1
ARC Centre of Excellence for Nanoscale BioPhotonics, Department of Physics and Astronomy, Macquarie University, Sydney, NSW 2109, Australia
2
School of Engineering, Macquarie University, Sydney, NSW 2122, Australia
3
ARC Centre of Excellence for Nanoscale BioPhotonics, Department of Molecular Sciences, Macquarie University, Sydney, NSW 2109, Australia
*
Authors to whom correspondence should be addressed.
Micromachines 2020, 11(1), 94; https://doi.org/10.3390/mi11010094
Received: 20 December 2019 / Accepted: 13 January 2020 / Published: 15 January 2020
(This article belongs to the Special Issue Microfluidic for High-Throughput Screening)
Single-cell analysis is of critical importance in revealing cell-to-cell heterogeneity by characterizing individual cells and identifying minority sub-populations of interest. Droplet-based microfluidics has been widely used in the past decade to achieve high-throughput single-cell analysis. However, to maximize the proportion of single-cell emulsification is challenging due to cell sedimentation and aggregation. The purpose of this study was to investigate the influence of single-cell encapsulation and incubation through the use of neutral buoyancy. As a proof of concept, OptiPrep™ was used to create neutrally buoyant cell suspensions of THP-1, a human monocytic leukemia cell line, for single-cell encapsulation and incubation. We found that using a neutrally buoyant suspension greatly increased the efficiency of single-cell encapsulation in microdroplets and eliminated unnecessary cell loss. Moreover, the presence of OptiPrep™ was shown to not affect cellular viability. This method significantly improved the effectiveness of single-cell study in a non-toxic environment and is expected to broadly facilitate single-cell analysis. View Full-Text
Keywords: single-cell analysis; droplet microfluidics; encapsulation efficiency; OptiPrep™; neutral buoyancy single-cell analysis; droplet microfluidics; encapsulation efficiency; OptiPrep™; neutral buoyancy
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Liu, H.; Li, M.; Wang, Y.; Piper, J.; Jiang, L. Improving Single-Cell Encapsulation Efficiency and Reliability through Neutral Buoyancy of Suspension. Micromachines 2020, 11, 94.

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