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Open AccessArticle

A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor

Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Beijing 100864, China
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Micromachines 2019, 10(10), 685; https://doi.org/10.3390/mi10100685 (registering DOI)
Received: 22 August 2019 / Revised: 29 September 2019 / Accepted: 7 October 2019 / Published: 11 October 2019
(This article belongs to the Special Issue Microfluidic for High-Throughput Screening)
A self-activated micropump which is capable of stable velocity transport for a liquid to flow a given distance inside a 3D microchannel has been a dream of microfluidic scientists for a long time. A new self-activated pumping mechanism has been proposed in this paper. It is different from the authors’ previous research which relied on the fluid resistance of a quartz capillary tube or end-blocked gas-permeable silicone or a polydimethylsiloxane (PDMS) wall to automate the flow. In this research, an end-open stretched Teflon tube was utilized for passive transport for the first time. A new fluid transmission mode was adopted with the assistance of a cheaper easily accessible oil mixture to achieve stable continuous flow. Finally, this novel micropump has been applied to real-time continuous-flow polymerase chain reactions (PCRs), with an amplification efficiency similar to that of a commercial PCR cycler instrument. View Full-Text
Keywords: microfluidics; viscosity; continuous flow; real-time PCR microfluidics; viscosity; continuous flow; real-time PCR
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MDPI and ACS Style

Wang, K.; Wu, D.; Wu, W. A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor. Micromachines 2019, 10, 685.

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