LC-MS/MS Analysis for Plant Secondary Metabolites

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Plant Metabolism".

Deadline for manuscript submissions: 20 September 2024 | Viewed by 779

Special Issue Editors


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Guest Editor
Department of Chemistry, Wuhan University, Wuhan, China
Interests: LC-MS analysis; metabolomics; nucleic acid modifications
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China
Interests: proteomics; metabolomics; multi-omics; cancer research; precision medicine; interaction networks

Special Issue Information

Dear Colleagues,

Plant secondary metabolites encompass a diverse group of molecules that play a critical role, yet they are not directly involved in plant growth, development, or stress response. Accurate and reliable analysis of the secondary metabolites would enhance our comprehension of their physiological status and deepen our understanding of specific biochemical processes in plants. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) has emerged as a powerful and essential technique for metabolomics analysis. Advancements in LC-MS/MS have facilitated the simultaneous measurement of numerous metabolites. Nevertheless, obtaining high-quality metabolomics datasets as well as effectively integrating and interpreting the data still present challenges.

In this Special Issue on "LC-MS/MS Analysis for Plant Secondary Metabolites”, we aim to showcase the wide range of research and applications of LC-MS/MS analysis in the field of plant secondary metabolites. We invite submissions covering various aspects of LC-MS/MS analysis, including applications, method and instrumentation development, and advancements in bioinformatic methodology. Original research papers as well as review articles are welcomed for submission.

Dr. Tiantian Ye
Dr. Lin Li
Guest Editors

Manuscript Submission Information

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Keywords

  • liquid chromatography
  • mass spectrometry
  • secondary metabolites
  • metabolomics
  • abiotic and biotic stress
  • plant development

Published Papers (1 paper)

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Research

21 pages, 1326 KiB  
Article
Metabolite Profiling Analysis of the Tongmai Sini Decoction in Rats after Oral Administration through UHPLC-Q-Exactive-MS/MS
by Xianhui Zheng, Yingying Zhan, Mengling Peng, Wen Xu and Guanghai Deng
Metabolites 2024, 14(6), 333; https://doi.org/10.3390/metabo14060333 - 14 Jun 2024
Viewed by 318
Abstract
Tongmai Sini decoction (TSD), the classical prescriptions of traditional Chinese medicine, consisting of three commonly used herbal medicines, has been widely applied for the treatment of myocardial infarction and heart failure. However, the absorbed components and their metabolism in vivo of TSD still [...] Read more.
Tongmai Sini decoction (TSD), the classical prescriptions of traditional Chinese medicine, consisting of three commonly used herbal medicines, has been widely applied for the treatment of myocardial infarction and heart failure. However, the absorbed components and their metabolism in vivo of TSD still remain unknown. In this study, a reliable and effective method using ultra-performance liquid chromatography coupled with hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Exactive-MS/MS) was employed to identify prototype components and metabolites in vivo (rat plasma and urine). Combined with mass defect filtering (MDF), dynamic background subtraction (DBS), and neutral loss filtering (NLF) data-mining tools, a total of thirty-two major compounds were selected and investigated for their metabolism in vivo. As a result, a total of 82 prototype compounds were identified or tentatively characterized in vivo, including 41 alkaloids, 35 phenolic compounds, 6 saponins. Meanwhile, A total of 65 metabolites (40 alkaloids and 25 phenolic compounds) were tentatively identified. The metabolic reactions were mainly hydrogenation, demethylation, hydroxylation, hydration, methylation, deoxylation, and sulfation. These findings will be beneficial for an in-depth understanding of the pharmacological mechanism and pharmacodynamic substance basis of TSD. Full article
(This article belongs to the Special Issue LC-MS/MS Analysis for Plant Secondary Metabolites)
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