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Horticulturae
Special Issues
In Vitro Conservation and Rapid Propagation of Horticultural Crops
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In Vitro Conservation and Rapid Propagation of Horticultural Crops

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Propagation and Seeds".

Deadline for manuscript submissions: 10 June 2026 | Viewed by 1928

Special Issue Editors

Dr. Antonio Bernabé-Antonio

E-Mail Website
Guest Editor
Department of Wood, Pulp and Paper, University Center for Exact Sciences and Engineering, University of Guadalajara, Km. 15.5, Carretera Guadalajara-Nogales, Col. Las Agujas, Zapopan 45020, Mexico
Interests: plant cell cultures; secondary metabolites; biological activity
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Francisco Cruz-Sosa

E-Mail Website
Guest Editor
Department of Biotechnology, Metropolitan Autonomous University, Iztapalapa Campus, Av. Ferrocarril de San Rafael Atlixco 186, Mexico City 09310, Mexico
Interests: plant cell cultures; secondary metabolites; biological activity
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The in vitro conservation and rapid propagation of horticultural crops have become essential biotechnological strategies for sustainable agriculture and the long-term preservation of plant genetic resources. These approaches provide major advantages, such as the reduction of space requirements and the maintenance of pathogen-free germplasm over extended periods. Such benefits are particularly relevant for species that are difficult to propagate through conventional methods, sterile hybrids, and elite cultivars. In the context of current global challenges, including climate change, food security concerns, and the continued loss of biodiversity, in vitro technologies play a critical role. They support the safe international exchange of germplasm, enable the large-scale production of certified plant material, and contribute to the conservation of traditional and threatened varieties. Moreover, they are indispensable tools in modern plant breeding programs and sustainable horticultural production systems, driving innovation in the conservation, multiplication, and improvement of economically and nutritionally valuable species.

This Special Issue aims to present recent advances, innovative methodologies, and technological improvements related to the in vitro conservation and propagation of horticultural species. In particular, we welcome submissions that explore novel species with demonstrated horticultural potential, including those exhibiting nutraceutical properties.

Dr. Antonio Bernabé-Antonio
Prof. Dr. Francisco Cruz-Sosa
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • horticultural crops
  • underutilized horticultural species
  • plant tissue culture
  • germplasm preservation
  • cryopreservation
  • sustainable horticulture
  • ex situ conservation
  • nutraceutical species

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Published Papers (1 paper)

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Research

15 pages, 1866 KB  
Article
Regulation on the Induction of Protocorm-like Bodies and Callus in Dendrobium officinale
by Ge-Ge Xu, Xiu-Mei Dong, Wei Chang and Shi-Bao Zhang
Horticulturae 2026, 12(5), 555; https://doi.org/10.3390/horticulturae12050555 - 2 May 2026
Viewed by 1429
Abstract
As globally important ornamental and medicinal plants, orchids exhibit significant differences in the difficulty and pathways of in vitro regeneration. Most orchid species can directly form protocorm-like bodies (PLBs) through the differentiation of shoot tips or other explants, which then regenerate into new [...] Read more.
As globally important ornamental and medicinal plants, orchids exhibit significant differences in the difficulty and pathways of in vitro regeneration. Most orchid species can directly form protocorm-like bodies (PLBs) through the differentiation of shoot tips or other explants, which then regenerate into new plantlets, while some species form callus through explant dedifferentiation followed by PLB differentiation from the callus. At present, the regenerative mechanisms underlying PLB and callus in orchids, as well as the key factors influencing their differentiation, remain poorly elucidated. In this study, seedlings of Dendrobium officinale obtained from aseptic seed germination were used to investigate the effects of explant type, 2,4-D concentration, temperature, light intensity and photoperiod on the induction of PLBs and callus. The results showed that there were no significant differences in callus induction among the tested explants in D. officinale, whereas stem nodal segments were more suitable for PLB induction. For both internodal and nodal segments, the incidence rate of callus formation was higher than that of PLBs. The concentration of 2,4-D influenced the induction direction of the explants; higher concentration promoted PLB induction, while lower concentration was sufficient for callus formation. Low temperature and low light intensity inhibited PLB induction while promoting callus formation in D. officinale. High temperature and intense light partially caused desiccation of explants. A temperature of 25/22 °C (day/night) and a photosynthetic photon flux density of 50 µmol m−2 s−1 were more suitable for callus or PLB induction in D. officinale. A shorter photoperiod favored callus induction, while a longer photoperiod was beneficial for PLB induction. This study reveals the differences in influencing factors for PLB and callus induction in D. officinale, providing important insights for the propagation of orchid seedlings and laying a significant foundation for elucidating the mechanisms of PLB and callus induction. Full article
(This article belongs to the Special Issue In Vitro Conservation and Rapid Propagation of Horticultural Crops)
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