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Coffee and Coffee-Related Product Characterization through Rapid Analytical Methodologies and...
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Coffee and Coffee-Related Product Characterization through Rapid Analytical Methodologies and Downscaled Systems

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: 28 June 2024 | Viewed by 1865

Special Issue Editors

Dr. João Rodrigo Santos

E-Mail Website
Guest Editor
REQUIMTE/LAQV—Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal
Interests: coffee analysis; volatile compound extraction and characterization; flow analysis; analytical chemistry; electrochemical detection; liquid chromatography; low-pressure chromatographic systems with amperometric detection
Special Issues, Collections and Topics in MDPI journals
Dr. Inês Maria Valente

E-Mail Website
Guest Editor
1. REQUIMTE/LAQV—Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Porto, Portugal
2. REQUIMTE, LAQV, ICBAS, School of Medicine and Biomedical Sciences, University of Porto, Porto, Portugal
Interests: analytical chemistry; phytochemical analysis; metabolomics; liquid chromatography; mass spectrometry; animal nutrition, method development; sample preparation
Special Issues, Collections and Topics in MDPI journals
Dr. Rui Miguel Ramos

E-Mail Website
Guest Editor
REQUIMTE/LAQV—Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal
Interests: volatile organic compounds; liquid chromatography; extraction methodologies; mass spectrometry; miniaturization; wood-based panels analysis; analytical chemistry; sample preparation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In view of the continuous analytical developments in terms of new sample preparation approaches and/or small-sized analytical instruments of competitive sensitivity, new analytical methods for physical and chemical characterization of coffee and coffee-related products are welcome. Such approaches are of special relevance for coffee roaster facilities which, due to poor/absent laboratorial conditions for routine analysis, choose not to perform proper analytical control of raw material, process control and final product. We hereby invite you to submit original research papers, review articles or short communications based on, but not limited to, the following topics: new analytical methodologies prone to be downsized for raw, roasted and brewed coffee of low cost; analytical methodologies focusing on compounds with known/ potential bioactivity, with an organoleptic impact, of legal concern or health-related.

Dr. João Rodrigo Santos
Dr. Inês Maria Valente
Dr. Rui Miguel Ramos
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • raw coffee, roasted coffee, brewed coffee and coffee by-products
  • coffee and coffee-related product quality assessment and characterization
  • compounds with potential bioactivity, organoleptic impact, of legal concern or health-related
  • portable, small-sized or easy-to-assemble analytical systems
  • rapid analytical methodologies for coffee and coffee-related products

Published Papers (2 papers)

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Research

15 pages, 3450 KiB  
Article
Exploring Microbial Influence on Flavor Development during Coffee Processing in Humid Subtropical Climate through Metagenetic–Metabolomics Analysis
by Alexander da Silva Vale, Cecília Marques Tenório Pereira, Juliano De Dea Lindner, Luiz Roberto Saldanha Rodrigues, Nájua Kêmil El Kadri, Maria Giovana Binder Pagnoncelli, Satinder Kaur Brar, Carlos Ricardo Soccol and Gilberto Vinícius de Melo Pereira
Foods 2024, 13(12), 1871; https://doi.org/10.3390/foods13121871 - 14 Jun 2024
Viewed by 306
Abstract
Research into microbial interactions during coffee processing is essential for developing new methods that adapt to climate change and improve flavor, thus enhancing the resilience and quality of global coffee production. This study aimed to investigate how microbial communities interact and contribute to [...] Read more.
Research into microbial interactions during coffee processing is essential for developing new methods that adapt to climate change and improve flavor, thus enhancing the resilience and quality of global coffee production. This study aimed to investigate how microbial communities interact and contribute to flavor development in coffee processing within humid subtropical climates. Employing Illumina sequencing for microbial dynamics analysis, and high-performance liquid chromatography (HPLC) integrated with gas chromatography–mass spectrometry (GC-MS) for metabolite assessment, the study revealed intricate microbial diversity and associated metabolic activities. Throughout the fermentation process, dominant microbial species included Enterobacter, Erwinia, Kluyvera, and Pantoea from the prokaryotic group, and Fusarium, Cladosporium, Kurtzmaniella, Leptosphaerulina, Neonectria, and Penicillium from the eukaryotic group. The key metabolites identified were ethanol, and lactic, acetic, and citric acids. Notably, the bacterial community plays a crucial role in flavor development by utilizing metabolic versatility to produce esters and alcohols, while plant-derived metabolites such as caffeine and linalool remain stable throughout the fermentation process. The undirected network analysis revealed 321 interactions among microbial species and key substances during the fermentation process, with Enterobacter, Kluyvera, and Serratia showing strong connections with sugar and various volatile compounds, such as hexanal, benzaldehyde, 3-methylbenzaldehyde, 2-butenal, and 4-heptenal. These interactions, including inhibitory effects by Fusarium and Cladosporium, suggest microbial adaptability to subtropical conditions, potentially influencing fermentation and coffee quality. The sensory analysis showed that the final beverage obtained a score of 80.83 ± 0.39, being classified as a specialty coffee by the Specialty Coffee Association (SCA) metrics. Nonetheless, further enhancements in acidity, body, and aftertaste could lead to a more balanced flavor profile. The findings of this research hold substantial implications for the coffee industry in humid subtropical regions, offering potential strategies to enhance flavor quality and consistency through controlled fermentation practices. Furthermore, this study contributes to the broader understanding of how microbial ecology interplays with environmental factors to influence food and beverage fermentation, a topic of growing interest in the context of climate change and sustainable agriculture. Full article
(This article belongs to the Special Issue Coffee and Coffee-Related Product Characterization through Rapid Analytical Methodologies and Downscaled Systems)
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22 pages, 3909 KiB  
Article
Formation of Cysteine Adducts with Chlorogenic Acid in Coffee Beans
by Sorel Tchewonpi Sagu, Nina Ulbrich, Johanna Rebekka Morche, Kapil Nichani, Haydar Özpinar, Steffen Schwarz, Andrea Henze, Sascha Rohn and Harshadrai M. Rawel
Foods 2024, 13(11), 1660; https://doi.org/10.3390/foods13111660 - 25 May 2024
Viewed by 878
Abstract
The post-harvest processing of coffee beans leads to a wide range of reactions involving proteins. The formation of crosslinks between proteins and phenolic compounds present in high concentrations of coffee beans represents one of the most challenging and still not fully characterized reactions. [...] Read more.
The post-harvest processing of coffee beans leads to a wide range of reactions involving proteins. The formation of crosslinks between proteins and phenolic compounds present in high concentrations of coffee beans represents one of the most challenging and still not fully characterized reactions. The aim of this work was to assess the presence of products from such reactions in coffee samples, focusing on the adducts between cysteine and chlorogenic acids (CQAs). For this purpose, 19 green and 15 roasted coffee samples of the Coffea arabica, Coffea canephora, and Coffea liberica varieties were selected for this study and basically characterized. Then, targeted liquid chromatography mass spectrometry (LC-MS/MS) methods were developed to assess the formation of adducts between CQA and cysteine, glutathione, and N-acetylcysteine as the amino acid and peptide models, and quantified such adducts in coffee samples. The results of the characterization showed a heterogeneous distribution of the protein content (8.7–14.6%), caffeine (0.57–2.62 g/100 g), and antioxidant capacity (2–4.5 g ascorbic acid/100 g) in Arabica, Canephora, and Liberica samples. Glutamic acid, arginine, and proline were found to be the major amino acids, while 5-CQA (38–76%), 3-CQA (4–13%), and 4-CQA (4–13%) were the most abundant CQA derivatives of all coffee varieties. The model experiments for adduct formation demonstrated that cysteine binds to CQA via thiol groups and 5-CQA initially isomerizes to 3- and 4-CQA, depending on the conditions, allowing cysteine to bind to two different sites on 3-, 4- or 5-CQA molecules, thus, forming six different Cys-CQA adducts with m/z 476. The reaction was more favored at pH 9, and the adducts proved to be stable up to 90 °C for 10 min and up to 28 days at room temperature. The relative quantification of adducts showed peak area values ranging from 1100 to 3000 in green coffee bean samples, while no adducts were detected in roasted coffee beans. Overall, this work was the first attempt to demonstrate the presence of Cys-CQA adducts in coffee beans and paves the way for further investigations of such adduct formation at the protein level. Full article
(This article belongs to the Special Issue Coffee and Coffee-Related Product Characterization through Rapid Analytical Methodologies and Downscaled Systems)
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