Plant-Derived Prebiotics and Their Role in Gut Health

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Nutrition".

Deadline for manuscript submissions: closed (15 April 2026) | Viewed by 1025

Editors


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Guest Editor
State Key Laboratory for Quality and Safety of Agro-Products & Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Interests: phytochemicals; dietary fiber; prebiotics; gut microbiota; microbiome metabolism; gut-brain axis; nutritional intervention; structure-activity relationship; functional foods

E-Mail Website
Guest Editor
State Key Laboratory for Quality and Safety of Agro-Products & Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Interests: gut microbiota in humans and animals; functional feed additives and dietary components; antimicrobial peptides and proteins
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
Interests: gut microbiota; marine polysaccharides; colitis; inflammatory bowel disease; fermentation; prebiotics; probiotics; obesity; diabetes; carbohydrate-based drugs
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This issue invites cutting-edge advances in the discovery, structural elucidation, and mechanistic understanding of plant-based prebiotic compounds. We welcome original research, reviews, and short communications on the following topics:

  • Novel oligosaccharides, polysaccharides, polyphenols, and other plant-based components with prebiotic properties.
  • Multi-omics insights into microbial fermentation kinetics, SCFA profiles, mucosal barrier reinforcement, and host immune crosstalk.
  • Robust in vitro, ex vivo, and in vivo models evaluating physiological outcomes relevant to obesity, diabetes, IBD, and neuro-inflammation.

This issue also encourages submissions on next-generation prebiotics, synergistic effects with probiotics, and sustainable strategies for prebiotic extraction and utilization. We seek to provide a platform highlighting scientific and practical insights into plant prebiotics for improving gut and overall health.

Dr. Xiaoqiong Li
Dr. Xin Wang
Dr. Qingsen Shang
Guest Editors

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Keywords

  • prebiotic
  • next-generation prebiotic
  • oligosaccharides
  • polysaccharides
  • polyphenols
  • synbiotic
  • gut microbiota
  • functional food

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Published Papers (1 paper)

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Research

24 pages, 5308 KB  
Article
Prebiotic Potential of Oligosaccharides and Polysaccharides Extracted from Leucaena leucocephala Seeds
by Viviane da Silva Sousa Almeida, Amanda Graziela Gonçalves Mendes, Carmem Duarte Lima Campos, Laís Araújo Souza Wolff, Ariadina Jansen Campos Fontes, José Lima Pereira-Filho, Taynara Figueiredo Costa, Cinara Regina Aragão Vieira Monteiro, Alan Silva de Menezes, Harvey Alexander Villa Vélez, Kátia Danielle Araújo Lourenço Viana and Valério Monteiro-Neto
Foods 2026, 15(11), 1890; https://doi.org/10.3390/foods15111890 - 27 May 2026
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Abstract
Background/Objectives: Plant-derived soluble fibers are being explored as sustainable prebiotic ingredients; however, tropical legumes such as Leucaena leucocephala remain understudied. This study evaluated soluble fibers from L. leucocephala seeds after simulated gastrointestinal digestion, focusing on rheological properties, microbial selectivity, metabolite production, and intestinal [...] Read more.
Background/Objectives: Plant-derived soluble fibers are being explored as sustainable prebiotic ingredients; however, tropical legumes such as Leucaena leucocephala remain understudied. This study evaluated soluble fibers from L. leucocephala seeds after simulated gastrointestinal digestion, focusing on rheological properties, microbial selectivity, metabolite production, and intestinal safety. Methods: The anatomical parts of the seed underwent INFOGEST 2.0 digestion. Soluble fibers were characterized by GC-MS monosaccharide profiling, viscosity, and SEM/EDS analyses, and were used as substrates for both probiotic and pathogenic bacteria. Fermentation supernatants were analyzed for short-chain fatty acids and lactate, and cytotoxicity was assessed using Caco-2 cells. Results: Endosperm polysaccharides exhibited high apparent viscosity (>300 cP) and pseudoplastic behavior. Monosaccharide profiles revealed the presence of galacto-oligosaccharides and arabinoxylo-oligosaccharides in the oligosaccharide fraction, and galactomannans, xylans, and arabinoxylans in the polysaccharide fraction. Polysaccharides selectively promoted the growth of Lacticaseibacillus rhamnosus GG and Bifidobacterium spp., comparable to or exceeding that of fructo-oligosaccharides (p < 0.05), while limiting pathogenic bacteria. Fermentation produced acetate and lactate concentrations of >4500 ppm and >1000 ppm, respectively. Caco-2 viability remained >90% across all treatments. Conclusions: Compartment-resolved analysis identified the endosperm as the principal source of digestion-resistant viscous fiber, selectively fermented by probiotic bacteria at levels matching or exceeding fructo-oligosaccharides. These findings position L. leucocephala endosperm fiber as a candidate prebiotic substrate, warranting further preclinical evaluation. Full article
(This article belongs to the Special Issue Plant-Derived Prebiotics and Their Role in Gut Health)
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