Fungi, Spoilage, and Food Safety

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (20 March 2024) | Viewed by 1007

Special Issue Editor


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Guest Editor
Departamento de Medicina Preventiva y Salud Pública, Ciencias de la Alimentación, Bromatología, Toxicología y Medicina Legal, Universitat de València, Vicente Andrès Estellès s/n, Burjassot, 46100 Valencia, Spain
Interests: fungi of food interest; mycotoxin-producing fungi; molecular identification; taxonomy; DNA-base methods; antifungal activity; antifungal proteins and peptides

Special Issue Information

Dear Colleagues,

Fungi are the major food contaminants in the world. They can grow on a wide diversity of crops including cereals, fruits, and vegetables. The proliferation of fungi in field crops and post-harvest products leads to reductions in yield and quality, with significant economic losses. Fungal spoilage can also occur in processed food like bread, wine, juice, fruit puree, jams, meat, and cheese, among other products. Within processed food, the development of mycelia, off-flavors and off-aromas, gases, and turbidity are characteristic of fungal spoilage, which can result in a product of unacceptable quality. Another major concern related to the fungal contamination of food commodities is the production of mycotoxins. They are toxic secondary metabolites produced by some fungi in crops or during the storage, transport, and processing of food and feed commodities, and they pose serious health risks for both humans and animals. A wide range of physical, chemical, and biological methods are applied to control fungal contamination, including green and emerging technologies such as ionizing and non-ionizing radiation, cold plasma, pulsed light, ultrasound, pulsed electric field, and high-pressure processing. Among them, the use of antifungal compounds continues to be one of the most effective strategies, including fungicides, chemical preservatives, and natural products. Molecular methods like PCR are used to detect and quantify the responsible fungi of food spoilage and mycotoxin production. There are also a wide range of detection and quantification methods for mycotoxins, including chromatography, new technologies using antibodies such as immunoassays, immunosensors, and lateral flow devices, as well as other bioanalytical, electrochemical, and optical techniques. This Special Issue welcomes research on preservation and detection methods available for fungi and innovative analytical methods for the determination of mycotoxins. In addition to classic methods, various innovative methods can significantly improve food safety. Tools and measures to prevent fungi, food spoilage, and mycotoxin contamination are welcome for this Special Issue. Recent research developments on fungi and food spoilage and mycotoxin contamination are also very welcome.

Dr. Pedro Vicente Martínez-Culebras
Guest Editor

Manuscript Submission Information

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Keywords

  • fungi
  • food spoilage
  • food safety
  • mycotoxin, mycotoxin-producing fungi
  • molecular identification
  • detection methods

Published Papers (1 paper)

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Research

15 pages, 3560 KiB  
Article
Fungal Mycobiome of Mature Strawberry Fruits (Fragaria x ananassa Variety ‘Monterey’) Suggests a Potential Market Site Contamination with Harmful Yeasts
by Gabriela N. Tenea, Pamela Reyes and Diana Molina
Foods 2024, 13(8), 1175; https://doi.org/10.3390/foods13081175 - 12 Apr 2024
Viewed by 689
Abstract
An amplicon metagenomic approach based on the ITS2 region of fungal rDNA was used to investigate the diversity of fungi associated with mature strawberries collected from a volcanic orchard and open-air market stands. Based on the Kruskal–Wallis test, no statistically significant differences were [...] Read more.
An amplicon metagenomic approach based on the ITS2 region of fungal rDNA was used to investigate the diversity of fungi associated with mature strawberries collected from a volcanic orchard and open-air market stands. Based on the Kruskal–Wallis test, no statistically significant differences were observed in both non-phylogenetic and phylogenetic alpha diversity indices. According to beta diversity analyses, significant differences in fungal communities were found between groups (orchard vs. market). Taxonomic assignment of amplicon sequence variables (ASVs) revealed 7 phyla and 31 classes. The prevalent fungal phyla were Basidiomycota (29.59–84.58%), Ascomycota (15.33–70.40%), and Fungi-phy-Insertae-sedis (0.45–2.89%). The most predominant classes among the groups were Saccharomycetes in the market group, and Microbotryomycetes and Tremellomycetes in the orchard group. Based on the analysis of microbiome composition (ANCOM), we found that the most differentially fungal genera were Hanseniaspora, Kurtzmaniella, and Phyllozyma. Endophytic yeasts Curvibasidium cygneicollum were prevalent in both groups, while Candida railenensis was detected in fruits originating only from the market. In addition, Rhodotorula graminis (relative abundance varying from 1.7% to 21.18%) and Papiliotrema flavescens (relative abundance varying from 1.58% to 16.55%) were detected in all samples regardless of origin, while Debaryomyces prosopidis was detected in samples from the market only, their relative abundance varying with the sample (from 0.80% to 19.23%). Their role in fruit quality and safety has not been yet documented. Moreover, several clinically related yeasts, such as Meyerozyma guilliermondii and Candida parapsilosis, were detected in samples only from the market. Understanding the variety and makeup of the mycobiome in ripe fruits during the transition from the orchard to the market is crucial for fruit safety after harvest. Full article
(This article belongs to the Special Issue Fungi, Spoilage, and Food Safety)
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