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Fishes
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Recent Advances in Crayfish
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Recent Advances in Crayfish

A special issue of Fishes (ISSN 2410-3888). This special issue belongs to the section "Aquatic Invertebrates".

Deadline for manuscript submissions: 10 August 2026 | Viewed by 2051

Special Issue Editors

Prof. Dr. Aimin Wang

E-Mail Website
Guest Editor
College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China
Interests: gut microbiota; crayfish nutrition; aquaculture; oxidative stress; nutritional physiology
Dr. Chunnuan Zhang

E-Mail Website
Guest Editor
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China
Interests: aquatic nutrition; oxidative stress; lipid metabolism; environmental pollutants

Special Issue Information

Dear Colleagues,

Crayfish represent one of the most valuable and globally cultivated freshwater crustaceans, with significant economic, ecological, and nutritional importance. Despite their prominence, the sustainable expansion of crayfish aquaculture faces multifaceted challenges, including optimizing nutrition and feed efficiency, enhancing disease resistance, improving reproductive performance, and minimizing environmental impact. This Special Issue, "Recent Advances in Crayfish", aims to compile high-quality original research and comprehensive reviews that address these critical areas. We welcome submissions exploring innovative aspects of crayfish biology and aquaculture, including, but not limited to, nutritional physiology and feed development, immunological responses and health management, genetic improvement and reproduction, welfare and behavior, aquaculture system engineering, and environmental sustainability. By disseminating the latest scientific insights and technological innovations, this collection seeks to contribute to the development of more efficient, resilient, and eco-friendly practices for the future of crayfish production. This theme will cover a wider range of research areas, including

  • Nutrition and feed development;
  • Physiology and metabolic regulation;
  • Health management and disease control;
  • Reproduction and genetic breeding;
  • Aquaculture systems and environmental sustainability;
  • Product quality and food safety.

Prof. Dr. Aimin Wang
Dr. Chunnuan Zhang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fishes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • crayfish
  • nutrition
  • physiology
  • health management
  • disease
  • reproduction

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

18 pages, 11322 KB  
Article
Effects of Different Attractants on Growth, Antioxidant Capacity, and Feeding Gene Expression of Procambarus clarkii
by Youhai Du, Silei Xia, Wuxiao Zhang, Wenping Yang, Yebing Yu, Zhaoxia Li, Bin Peng, Yude Wang, Bo Liu, Hongyan Tian and Jianhua Ming
Fishes 2026, 11(5), 267; https://doi.org/10.3390/fishes11050267 - 30 Apr 2026
Viewed by 393
Abstract
The purpose of this study was to investigate the effects of different feeding-promoting substances added to high plant protein diets on the growth, antioxidant, serum biochemical parameters, immune, and feeding-related genes of Procambarus clarkii. A total of 450 crayfish (3.94 ± 0.03 [...] Read more.
The purpose of this study was to investigate the effects of different feeding-promoting substances added to high plant protein diets on the growth, antioxidant, serum biochemical parameters, immune, and feeding-related genes of Procambarus clarkii. A total of 450 crayfish (3.94 ± 0.03 g) were selected and randomly divided into six groups, with each group consisting of three replicates and 25 crayfish per replicate. The crayfish were fed a basal diet without attractant (control group) and five experimental diets supplemented with 0.4% betaine (BET), 0.4% trimetlylamine oxide (TMAO), 0.4% squid paste (SQU), 0.4% dimethyl-β-propiothetin (DMPT), and 0.4% taurine (TAU). The feeding trial lasted for 6 weeks. The results showed that compared with the control group, the BET, SQU, DMPT, and TAU groups significantly improved in growth performance, weight gain rate, and specific growth rate of crayfish. Compared with the control group, the BET, MTAO, and SQU groups significantly increased hepatopancreas SOD, CAT, and T-AOC. Histological results showed that compared with the control group, all feeding attractant groups could alleviate hepatopancreas tissue damage. Compared with the control group, the TMAO and SQU groups significantly reduced serum GLU content as well as ACP and AKP activities. The results of gene quantitative analysis showed that, compared with the control, TMAO significantly upregulated the expression of tlr, nf-kb, propo, hsp70, and tgf-β, while TAU significantly increased the expression of hsp70, hsp90 and nf-kb genes. Compared with the control group, the expression levels of tor, 4ebp1, and s6k1 in the TMAO group were significantly increased. Compared with the control group, the expression levels of leptin and npy genes in the DMPT group were significantly increased. In summary, the addition of attractants to high plant protein feed has the effects of promoting growth, enhancing antioxidant capacity, improving digestive enzyme activity, alleviating hepatopancreas injury, improving immunity, and promoting feeding. Full article
(This article belongs to the Special Issue Recent Advances in Crayfish)
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21 pages, 2347 KB  
Article
Effects of Dietary Tryptophan on Growth Performance, Muscle Development and Quality, Gut Microbiota of Juvenile Procambarus clarkii
by Ying Chen, Ling Zhu, Hanwu Wu, Yebing Yu, Xiaochuan Zheng, Bo Liu, Cunxin Sun, Xuwen Bing, Hongyan Tian, Ejaz Naqeebullah, Sharifi Saifullah, Yongfeng Zhao and Bo Liu
Fishes 2026, 11(3), 188; https://doi.org/10.3390/fishes11030188 - 20 Mar 2026
Viewed by 484
Abstract
This study aimed to investigate the effects of dietary tryptophan (Trp) on growth performance and muscle quality of Procambarus clarkii. Six experimental diets with graded Trp concentrations (0.05%, 0.13%, 0.29%, 0.43%, 0.56%, 0.69%; designated Trp0.05 to Trp0.69) were fed to crayfish for [...] Read more.
This study aimed to investigate the effects of dietary tryptophan (Trp) on growth performance and muscle quality of Procambarus clarkii. Six experimental diets with graded Trp concentrations (0.05%, 0.13%, 0.29%, 0.43%, 0.56%, 0.69%; designated Trp0.05 to Trp0.69) were fed to crayfish for 8 weeks. Growth parameters, muscle proximate composition, texture, histology, related gene expression, and intestinal microbiota were measured. Compared with the Trp0.05 group, the Trp0.43 group significantly increased FW, WGR, SGR, and muscle crude protein content, while decreasing FCR. It also improved muscle texture (hardness, springiness, cohesiveness, gumminess, chewiness), increased muscle fiber diameter, and reduced fiber density and the proportion of fibers < 30 μm. Additionally, the Trp0.43 group upregulated mRNA expression of MEF2A, MEF2B, MLC1, MyHC, mTOR, S6K1, AKT, LARP6, Col1α1, Col1α2, TGF-β1, and Smad, and downregulated MSTN, 4EBP1, FOXO, and LC3. It reduced Proteobacteria and Shewanella abundance, and increased Bacteroidota and Firmicutes. In conclusion, appropriate dietary Trp improves P. clarkii growth, muscle quality, and intestinal microbiota. Based on quadratic regression analysis of WGR and SGR, the dietary Trp requirement of P. clarkii was estimated to be 0.39%, corresponding to 1.22% of feed protein. Full article
(This article belongs to the Special Issue Recent Advances in Crayfish)
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13 pages, 2270 KB  
Article
Molecular Insights into Exoskeletal Remodeling: Transcriptomic Profiling of the Molting Cycle in the Red Swamp Crayfish Procambarus clarkii
by Yunkuan Zhu, Yu Xu, Suwan Lu, Yuning Hu, Hui Xu, Jiajia Li, Hai Lin, Xuguang Li and Zhiqiang Xu
Fishes 2026, 11(3), 166; https://doi.org/10.3390/fishes11030166 - 13 Mar 2026
Viewed by 580
Abstract
The molting of crustaceans is accompanied by exoskeleton reconstruction. To reveal the molecular regulation mechanism of exoskeleton remodeling, the transcriptomic profiles of the exoskeleton across the entire molting process in the red swamp crayfish Procambarus clarkii were investigated by RNA sequencing, yielding a [...] Read more.
The molting of crustaceans is accompanied by exoskeleton reconstruction. To reveal the molecular regulation mechanism of exoskeleton remodeling, the transcriptomic profiles of the exoskeleton across the entire molting process in the red swamp crayfish Procambarus clarkii were investigated by RNA sequencing, yielding a total of 7671 differentially expressed genes (DEGs) across five different molting stages. Notably, the key DEGs were those related to cuticular exoskeleton synthesis (cuticular proteins), degradation (chitinase 2, chitinase 10) and hardening (chitin deacetylase 1), and their expression abundance varied by 10-fold or greater across the molting cycle. Analysis of Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that significantly enriched pathways included the structural constituents of the cuticle, structural molecule activity, chitin binding of chitin metabolism, and hormone biosynthesis. The expression profiles of nine selected molting-related DEGs were further validated via real-time RT-PCR assays. The acquired unique temporal expression patterns involved in exoskeleton remodeling provide a preliminary insight into the regulation of gene expression during the molting cycle in the red swamp crayfish. Full article
(This article belongs to the Special Issue Recent Advances in Crayfish)
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