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Clean Technol.
Special Issues
Sustainable Biofuel and Biochemical Production from Lignocellulosic Biomass
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Sustainable Biofuel and Biochemical Production from Lignocellulosic Biomass

A special issue of Clean Technologies (ISSN 2571-8797).

Deadline for manuscript submissions: 20 December 2025 | Viewed by 1694

Special Issue Editors

Dr. Nenad Marđetko

E-Mail Website
Guest Editor
Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Interests: lignocellulose pretreatment; enzymes; bioprocess engineering; fungi; biochemicals
Dr. Antonija Trontel

E-Mail Website
Guest Editor
Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Interests: bioprocess; biotechnology; biochemical engineering; bioprocess engineering and fermentation technology; bioreactors; bioprocess engineering; fermentation
Dr. Mario Novak

E-Mail Website
Guest Editor
Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Interests: biofuels production; sustainable development; bioprocess engineering; yeast; mathematical modeling; beer technology; biochemicals production from renewable materials

Special Issue Information

Dear Colleagues,

The terms lignocellulosic materials and lignocellulosic biomass refer to plant biomass that can originate from various sources. Lignocellulose makes up about 60% of the biomass produced by plants on Earth, and there are numerous possibilities for its biotechnological applications. Lignocellulose consists of cellulose, hemicellulose, and lignin, which can be broken down into simpler components through different pretreatment methods. This conversion turns the biomass into a valuable feedstock for various biotechnological processes. The resulting biofuels (e.g., bioethanol) and biochemicals offer a more sustainable and environmentally friendly option, reducing greenhouse gas emissions and dependence on non-renewable resources. It is essential to develop new efficient processes in which these materials can be used as renewable raw materials while simultaneously creating opportunities for the production of high-value products. Thus, lignocellulose can become the foundation of production processes that are justified from both ecological and economic standpoints.

Dr. Nenad Marđetko
Dr. Antonija Trontel
Dr. Mario Novak
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Clean Technologies is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • lignocellulosic biomass
  • biofuels
  • biochemicals
  • pretreatment methods
  • fermentation
  • circular economy
  • waste valorization

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Published Papers (2 papers)

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Research

21 pages, 1828 KiB  
Article
Evaluation of Industrial Wastewaters as Low-Cost Resources for Sustainable Enzyme Production by Bacillus Species
by Vu_Mai_Linh Nguyen, Adama Ndao, Jean-François Blais and Kokou Adjallé
Clean Technol. 2025, 7(2), 45; https://doi.org/10.3390/cleantechnol7020045 - 31 May 2025
Viewed by 194
Abstract
The increasing demand for industrial enzymes calls for cost-effective and sustainable production strategies. This study investigates the potential of industrial wastewater as an alternative fermentation medium for enzyme synthesis, aligning with the principles of the circular bioeconomy. Four wastewater types from Québec, Canada—beverage [...] Read more.
The increasing demand for industrial enzymes calls for cost-effective and sustainable production strategies. This study investigates the potential of industrial wastewater as an alternative fermentation medium for enzyme synthesis, aligning with the principles of the circular bioeconomy. Four wastewater types from Québec, Canada—beverage wastewater (BW), pulp and paper mill activated sludge (PPMS), food industry wastewater (FIW), and starch industry wastewater (SIW)—were evaluated for their potential to support protease, amylase, and lipase production using Bacillus licheniformis, Bacillus amyloliquefaciens, and Bacillus megaterium. Initial screening identified SIW as optimal for amylase production with B. amyloliquefaciens, and PPMS for protease production with B. megaterium. Optimization using the Box–Behnken design was then performed, followed by scale-up experiments in 5 L bioreactors. B. amyloliquefaciens achieved 5.73 ± 0.01 U/mL of amylase at 48 h under 40 g/L total solids, 30 °C, and a 2% inoculum size, while B. megaterium produced the highest protease of 55.41 ± 3.54 U/mL at 24 h. Lipase production remained negligible across all media and strains. These findings demonstrate the feasibility of the potential of wastewater-based enzyme production, reducing reliance on expensive synthetic substrates, mitigating environmental burdens, and contributing to the transition to a circular bioeconomy. Full article
(This article belongs to the Special Issue Sustainable Biofuel and Biochemical Production from Lignocellulosic Biomass)
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23 pages, 6146 KiB  
Article
Design and Implementation of a Low-Pressure Briquetting Machine for the Use of Pinus spp. Wood Residues: An Approach to Appropriate Rural Technology
by Mario Morales-Máximo, Víctor Manuel Ruíz-García, José Guadalupe Rutiaga-Quiñones and Luis Bernado López-Sosa
Clean Technol. 2025, 7(1), 22; https://doi.org/10.3390/cleantechnol7010022 - 6 Mar 2025
Viewed by 1087
Abstract
This research analyzes the technical feasibility and implementation of an appropriate technology for the production of briquettes from Pinus spp. waste (sawdust and shavings) in a rural community in Michoacán, Mexico. The results indicate that local small-scale briquette production in the Pichátaro community [...] Read more.
This research analyzes the technical feasibility and implementation of an appropriate technology for the production of briquettes from Pinus spp. waste (sawdust and shavings) in a rural community in Michoacán, Mexico. The results indicate that local small-scale briquette production in the Pichátaro community has the potential to boost a local economy based on the manufacturing and marketing of densified solid biofuels. The design of the manual briquetting machine was developed through a participatory approach with community users. Structural simplicity and locally accessible maintenance were prioritized, the aspects that were addressed little in previous studies. The machine allows for the production of briquettes using a low-cost mixture composed of sawdust and Pinus spp. shavings, corn starch, and water. Based on local conditions and production needs, parameters such as reduced processing times and simplified manufacturing methods were identified as essential to establishing an efficient regional production and supply chain. Furthermore, the valorization of solid waste through the production of alternative biofuels contributes to the diversification of the energy matrix in rural residential sectors and small industries in communities in Mexico. The estimated cost of the machine is USD 75.44, and most of its components are easily replaceable, which favors its sustainability and prolonged use. This study demonstrates that the implementation of a low-pressure briquette system based on appropriate rural technologies represents a viable strategy for the use of wood waste and the promotion of sustainable energy solutions in rural communities. Full article
(This article belongs to the Special Issue Sustainable Biofuel and Biochemical Production from Lignocellulosic Biomass)
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