Advancement in Optical Biosensor for Bioassay and Detection of New Pollutants

A special issue of Chemosensors (ISSN 2227-9040). This special issue belongs to the section "Optical Chemical Sensors".

Deadline for manuscript submissions: 30 September 2024 | Viewed by 1802

Special Issue Editor


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Guest Editor
School of Environment and Natural Resource, Renmin University of China, Beijing 100872, China
Interests: environmental monitoring theory, technology, and equipment; optical biosensors and nanosensor; biological functional materials and nanomaterials

Special Issue Information

Dear Colleagues,

New pollutants (NPs) are toxic and harmful chemical substances discharged into the environment with biological toxicity, environmental persistence, and bioaccumulation. NPs refer to a broad spectrum of compounds applied in modern society, including but not limited to persistent organic pollutants (POPs), endocrine-disrupting chemicals (EDCs), antibiotics, microplastics, and other pollutants that have not been effectively regulated at the current stage. Because the chance of exposure to NPs is increasing, monitoring requirements are correspondingly increasing for understanding and managing their risks to human health and the environment. The rapid, high-frequency, and on-site/on-line quantitative detection of NPs is essential for early warning of pollution accident, reducing population mortality, and taking remedial action as and when the need arises.

Optical biosensors represent an attractive solution because they embrace great potential for highly sensitive and specific, real-time, high-frequency detection of pollutants in complex matrices with minimal sample preparation. This Special Issue provides a platform on the mechanisms for optical biosensing and the recent development of bioreceptors that enhance the rapid, easy, and accurate analysis of NPs. The advantages and challenges for the sensitivity, selectivity, and durability of optical biosensors are discussed, together with the opportunities and development strategies. Both original research papers, short communications, and review articles are welcome.

Prof. Dr. Feng Long
Guest Editor

Manuscript Submission Information

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Keywords

  • new pollutants
  • optical biosensors
  • persistent organic pollutants
  • endocrine-disrupting chemicals
  • antibiotics
  • on-site detection
  • early-warning

Published Papers (1 paper)

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Research

13 pages, 2319 KiB  
Article
Rapid and Sensitive On-Site Detection of Fipronil in Foods Using Evanescent Wave Fluorescent Immunosensor
by Yujing Li, Wenjuan Xu, Jingjing Liu, Erjing Zhang, Hejie Li, Yan Zhang, Jing Zhang, Chunsheng Li and Xiaoguang Zhang
Chemosensors 2023, 11(12), 578; https://doi.org/10.3390/chemosensors11120578 - 17 Dec 2023
Viewed by 1547
Abstract
Fipronil (FIP), a broad-spectrum phenylpyrazole insecticide, is highly toxic and threatens human health and ecological balance. Developing convenient, rapid, portable analytical technology for on-site and high-frequency testing of FIP is essential to reduce its damage. Herein, a monoclonal antibody (Clone F-3F6) against FIP, [...] Read more.
Fipronil (FIP), a broad-spectrum phenylpyrazole insecticide, is highly toxic and threatens human health and ecological balance. Developing convenient, rapid, portable analytical technology for on-site and high-frequency testing of FIP is essential to reduce its damage. Herein, a monoclonal antibody (Clone F-3F6) against FIP, with high affinity and specificity, was produced using a novel immunogen, FIP-BSA, which was simply and directly synthesized by conjugating FIP with bovine serum albumin (BSA). Among the previously reported antibodies, F-3F6 acts more specifically against FIP. The FIP metabolites fipronil desulfinyl, fipronil sulfide, and fipronil sulfone showed lower cross-reactivity, and other pesticides were not recognized. To achieve high-frequency and on-site measurements of FIP, an evanescent wave fluorescence biosensor was built by integrating evanescent wave fluorescence technology, a functionalized fiber bioprobe, and a fluorescence-labeled F-3F6 antibody. The detection limit of FIP was 0.032 μg/L. The detection results of real milk and water samples showed that all the coefficients of variation were less than 10%, and the recovery ranged from 90 to 120%. The high reusability and stability of functionalized fiber bioprobe enables the accurate, cost-effective, high-frequency, and facile quantitative detection of FIP. This highly specific and reliable evanescent wave fluorescence biosensor will be well suited to the sensitive and high-frequency on-site analysis of only FIP in food. Full article
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