Special Issue "Recent Advances in Intravital and Live Cell Imaging"

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cell Methods".

Deadline for manuscript submissions: 1 August 2023 | Viewed by 2470

Special Issue Editors

Cellular Stress and Ageing Program, Bionanoscience and Bioimaging Program, BNS, 33647 Bielefeld, Germany
Interests: quantum dots; bionanotechnology; two-photon fluorescence imaging; cellular imaging; fluorescence microscopy; cancer; cell signaling; oxidative stress; lipids and biomembranes; lipid peroxidation; antioxidants; renal pathobiology; extracellular vesicles; Super-resolution microscopy
Special Issues, Collections and Topics in MDPI journals
Walter Brendel Centre of Experimental Medicine, Department of Cardiology, Ludwig Maximilian University of Munich, Marchioninistraße 27, D-81377 München, Germany
Interests: multiphoton microscopy; intravital microscopy; confocal and super-resolution microscopy; fluorescent probes; leukocyte trafficking; atherosclerosis; inflammation; hematopoiesis and cytoskeleton proteins
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Ever since the invention of lenses, man has not only looked into the skies, but also into the realm of the “invisible” microscopic world of tissues, cells, and micro-organisms. The use of fluorescent labels and the development of various forms of optical microscopy, particularly confocal laser scanning microscopy (CLSM), super-resolution microscopy (SRM), and intravital microscopy (IVM), have significantly advanced our knowledge about the basic mechanisms underpinning biology and the pathophysiological processes that lead to disease.

This Special Issue focuses exclusively on intravital, super-resolution, and life cell microscopy techniques which are developed to study cellular and subcellular events in health and diseases.

Previously unpublished experimental, theoretical, prospective, and review papers (with the exception of preprint servers) are solicited on the following and related topics:

  • Intravital imaging of animal model tissues to study cellular dynamics in acute or chronic set up (longitudinal imaging);
  • Generation of novel animal models to study cellular and subcellular events;
  • Recent advances in intravital imaging technology to study cellular dynamics behavior in vivo for instance through an imaging window or optical fibers;
  • Advances in super-resolution microscopy technologies;
  • New developments in life cell imaging in general;
  • Advances in fluorescence microscopy techniques, such as FRET, FLIM, FRAP, FLIP et al., with respect to life cell imaging;
  • Correlative microscopy;
  • Fundamental physicochemical properties, synthesis, and modification of novel fluorophores for imaging.

Dr. Gregor Drummen
Dr. Hellen Ishikawa-Ankerhold
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescence probe
  • fluorophore
  • fluorescence microscopy
  • super-resolution microscopy
  • nanoscopy
  • confocal laser scanning microscopy
  • two-photon microscopy
  • FRET
  • FLIM
  • FRAP
  • live cell imaging
  • intravital microscopy
  • longitudinal imaging techniques, imaging window
  • animal models for intravital imaging
  • multimodal imaging

Published Papers (2 papers)

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Research

Article
Cell Surface Charge Mapping Using a Microelectrode Array on ITO Substrate
Cells 2023, 12(4), 518; https://doi.org/10.3390/cells12040518 - 04 Feb 2023
Viewed by 818
Abstract
Many cellular functions are regulated by cell surface charges, such as intercellular signaling and metabolism. Noninvasive measurement of surface charge distribution of a single cell plays a vital role in understanding cellular functions via cell membranes. We report a method for cell surface [...] Read more.
Many cellular functions are regulated by cell surface charges, such as intercellular signaling and metabolism. Noninvasive measurement of surface charge distribution of a single cell plays a vital role in understanding cellular functions via cell membranes. We report a method for cell surface charge mapping via photoelectric interactions. A cell is placed on an array of microelectrodes fabricated on a transparent ITO (indium tin oxide) surface. An incident light irradiates the ITO surface from the backside. Because of the influence of the cell surface charge (or zeta potential), the photocurrent and the absorption of the incident light are changed, inducing a magnitude change of the reflected light. Hence, the cell surface charge distribution can be quantified by analyzing the reflected light intensity. This method does not need physical or chemical modification of the cell surface. We validated this method using charged microparticles (MPs) and two types of cells, i.e., human dermal fibroblast cells (HDFs) and human mesenchymal stem cells (hMSC). The measured average zeta potentials were in good agreement with the standard electrophoresis light scattering method. Full article
(This article belongs to the Special Issue Recent Advances in Intravital and Live Cell Imaging)
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Article
IL18 Receptor Signaling Inhibits Intratumoral CD8+ T-Cell Migration in a Murine Pancreatic Cancer Model
Cells 2023, 12(3), 456; https://doi.org/10.3390/cells12030456 - 31 Jan 2023
Cited by 1 | Viewed by 1067
Abstract
In pancreatic ductal adenocarcinoma (PDAC), the infiltration of CD8+ cytotoxic T cells (CTLs) is an important factor in determining prognosis. The migration pattern and interaction behavior of intratumoral CTLs are pivotal to tumor rejection. NLRP3-dependent proinflammatory cytokines IL-1β and IL-18 play a [...] Read more.
In pancreatic ductal adenocarcinoma (PDAC), the infiltration of CD8+ cytotoxic T cells (CTLs) is an important factor in determining prognosis. The migration pattern and interaction behavior of intratumoral CTLs are pivotal to tumor rejection. NLRP3-dependent proinflammatory cytokines IL-1β and IL-18 play a prominent role for CTL induction and differentiation. Here, we investigate the effects of T-cellular IL-1R and IL-18R signaling for intratumoral T-cell motility. Murine adenocarcinoma cell line Panc02 was stably transfected with ovalbumin (OVA) and fluorophore H2B-Cerulean to generate PancOVA H2B-Cerulean tumor cells. Dorsal skinfold chambers (DSFC) were installed on wild-type mice, and PancOVA H2B-Cerulean tumor cells were implanted into the chambers. PancOVA spheroids were formed using the Corning® Matrigel®-based 3D cell culture technique. CTLs were generated from OT-1 mice, Il1r−/− OT-1 mice, or Il18r−/− OT-1 mice and were marked with fluorophores. This was followed by the adoptive transfer of CTLs into tumor-bearing mice or the application into tumor spheroids. After visualization with multiphoton microscopy (MPM), Imaris software was used to perform T-cell tracking. Imaris analysis indicates a significantly higher accumulation of Il18r−/− CTLs in PancOVA tumors and a significant reduction in tumor volume compared to wild-type CTLs. Il18r−/− CTLs covered a longer distance (track displacement length) in comparison to wild-type (WT) CTLs, and had a higher average speed (mean track speed). The analysis of instantaneous velocity suggests a higher percentage of arrested tracks (arrests: <4 μm/min) for Il18r−/− CTLs. Our data indicate the contribution of IL-18R signaling to T-cell effector strength, warranting further investigation on phenomena such as intratumoral T-cell exhaustion. Full article
(This article belongs to the Special Issue Recent Advances in Intravital and Live Cell Imaging)
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Novel in vivo Multiphoton Intravital Imaging of Murine Gastric Mucosa revels Leukocyte trafficking on Helicobacter pylori infection
Authors: Hellen Ishikawa-Ankerhold1,2, Benjamin Busch3, Sukumar Namineni3, Simona Nasiscionyte2, Mykhailo Vladymyrov4,5,6, Ute Breithaupt3, Dominic van del Heuvel1,2, Christian Schulz1, Steffen Massberg1 and
Affiliation: 1 Medizinische Klinik und Poliklinik I, Marchioninistr. 15, 81377 Munich, Germany 2 Walter Brendel Centre of Experimental Medicine, Marchioninistr. 27, 81377 Munich, Germany 3 Department of Bacteriology , Max von Pettenkofer Institute, Pettenkofer Straße 9a, 80336 Munich 4 Laboratory for High Energy Physics (LHEP), Albert Einstein Center for Fundamental Physics, University of Bern, Bern, Switzerland 5 Theodor Kocher Institute, University of Bern, Bern, Switzerland 6 Science IT Support, Mathematical Institute, University of Bern, Bern, Switzerland

Title: Candida albicans infection in the kidney: live imaging of early colonization events
Authors: Fitz Silao1, Biborka Bereczky-Veress1, Per Ljungdahl1 and Christiane Peuckert1
Affiliation: Stockholm University, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm, Sweden

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