Fluorophores - The Fluorescent Toolbox in Biological and Biomedical Research

Dear Colleagues,

Ever since the invention of lenses, man has not only looked into the skies, but also into the realm of the invisible microscopic world of tissues, cells, and micro-organisms. The use of fluorescent labels and the development of various forms of optical microscopy, in particular confocal laser scanning microscopy (CLSM), have significantly advanced our knowledge about the basic mechanisms underpinning biology and the pathophysiological processes that lead to disease. Furthermore, fluorescence-based assays have largely replayed radioactive assays in the lab.

It is intended that this Special Issue of “Molecules” will consider fundamental physicochemical properties, synthesis and modification, biomedical, imaging and assay applications of fluorophores, from organic dyes to fluorescent nanoparticles and fluorescent proteins. In this respect it should be stressed that the fluorescent molecule always takes centre stage. Previously unpublished experimental, theoretical, prospective, historical, and review papers are solicited on the following and related topics:

• Synthesis and modification of fluorophores
• Physico-chemical and fluorescent properties
• Biocompatibility and cytotoxicity
• Live-cell tracking and imaging
• Whole animal imaging
• FRET, FLIM, FRAP, FLIP et al.
• Applications in cell biology and (bio)medicine
• Fluorescence-based assays and biosensors

Dr. Gregor Drummen
Guest Editor