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Biosensors
Special Issues
Next-Generation Immunosensors
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Next-Generation Immunosensors

A special issue of Biosensors (ISSN 2079-6374).

Deadline for manuscript submissions: closed (31 July 2016) | Viewed by 21644

Special Issue Editor

Prof. Dr. Luigi Campanella

E-Mail Website
Guest Editor
Department of Chemistry, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy
Interests: glucose determination; chemical sensors engineering; wearable devices
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Immunosensors are analytical devices based on the formation of antigen–antibody complexes, which are detected and converted to a signal, generally of an optical, electrochemical, or piezoelectric nature. Different trasducing mechanisms are employed in immunosensors, based on signal generation or on property changes, due to the formation of the antigen/antibody complex.

Immunosensors are considered a special type of biosensor, aiming at the detection of the presence of specific antibodies or antigens, and is particularly important in the case of diagnosis of deseases, and, further, is more significant when performed in remote areas, when lacking trained analysis or required facilities in underdeveloped countries, where carrying out immunoessays, such as ELISA (Enzyme linked Immunosorbent Assay), is not possible.

Different disciplines converge in an immunosensor: biology, chemistry, electronics, and the improvements can play on one or more of these three areas. Thus, the idea of a Special Issue on these kinds of devices is related to the willingness to contribute to the dissemination of new opportunities, able to monitor and, consequently, to fight epidemics and their spread.

Another aspect of possible innovations is represented by the possibility to extend the media to which these tools are applied. Commonly, they are body fluids, especially serum, where antibodies or antigens are determined; however, increasing interest concerns the detection of different analytes in diverse media, such as groundwater and vegetal samples.

Finally, a weakness of immunosensors is represented by their single use, because of the high stability of the antigen–antibody complex. Thus, regeneration of these devices appears to be a significant and relevant innovation.

Prof. Dr. Luigi Campanella
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

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Published Papers (3 papers)

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1235 KiB  
Article
Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers
by María Pedrero, F. Javier Manuel de Villena, Cristina Muñoz-San Martín, Susana Campuzano, María Garranzo-Asensio, Rodrigo Barderas and José M. Pingarrón
Biosensors 2016, 6(4), 56; https://doi.org/10.3390/bios6040056 - 21 Nov 2016
Cited by 26 | Viewed by 7423
Abstract
An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture [...] Read more.
An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation. Full article
(This article belongs to the Special Issue Next-Generation Immunosensors)
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1127 KiB  
Article
Effects of Surface Epitope Coverage on the Sensitivity of Displacement Assays that Employ Modified Nanoparticles: Using Bisphenol A as a Model Analyte
by Yang Lu, Joshua Richard Peterson, Erwann Luais, John Justin Gooding and Nanju Alice Lee
Biosensors 2016, 6(3), 43; https://doi.org/10.3390/bios6030043 - 8 Aug 2016
Cited by 3 | Viewed by 6477
Abstract
With the ever-increasing use of nanoparticles in immunosensors, a fundamental study on the effect of epitope density is presented herein, with a small molecule epitope, on the performance of the displacement assay format in an enzyme-linked immunosorbent assay (ELISA). Thiolated bisphenol A (BPA) [...] Read more.
With the ever-increasing use of nanoparticles in immunosensors, a fundamental study on the effect of epitope density is presented herein, with a small molecule epitope, on the performance of the displacement assay format in an enzyme-linked immunosorbent assay (ELISA). Thiolated bisphenol A (BPA) functionalized gold nanoparticles (cysBPAv-AuNPs) and specific anti-BPA antibodies are employed for this purpose. It is shown that the displacement of cysBPAv-AuNPs bound to the immobilized antibodies was influenced by both the avidity of bound cysBPAv-AuNPs and the concentration of free BPA to displace it. The importance of surface epitope density was that it changed the number of epitopes in close proximity to the antibody-binding site. This then influenced the avidity of cysBPAv-AuNPs bound to the immobilized antibody. Furthermore, the molar epitope concentration in an assay appears to affect the degree of antibody binding site saturation. Controlling surface epitope density of the functionalized nanoparticles and molar epitope concentration in an assay leads to a decrease of the concentration of free BPA required to displace the bound cysBPAv-AuNP, and hence better assay performance with regards to the D50 value and dynamic range in the displacement assay. Full article
(This article belongs to the Special Issue Next-Generation Immunosensors)
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2784 KiB  
Article
A Flow SPR Immunosensor Based on a Sandwich Direct Method
by Mauro Tomassetti, Giorgia Conta, Luigi Campanella, Gabriele Favero, Gabriella Sanzò, Franco Mazzei and Riccarda Antiochia
Biosensors 2016, 6(2), 22; https://doi.org/10.3390/bios6020022 - 13 May 2016
Cited by 19 | Viewed by 7060
Abstract
In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) [...] Read more.
In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10−3 and 10−1 M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes. Full article
(This article belongs to the Special Issue Next-Generation Immunosensors)
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